Alteration of Interferon Gamma (IFN-γ) in Human Plasma with Graded Physical Activity
Introduction: Practice of regular exercise is beneficial for health. Physical exercises have been demonstrated to alter levels of the cytokine interferon Gamma in plasma. IFN-γ is known to be an anti-inflammatory cytokine. Materials and Methods: We assessed the effect of single bout of moderate...
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| Format: | Article |
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JCDR Research and Publications Private Limited
2014-06-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/4440/9502_CE(Ra1)_F(H)_PF1(PAK)_PFA(AK)_PF2(AGAK).pdf |
| _version_ | 1819019386199998464 |
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| author | Ambarish Vijayaraghava Radhika K |
| author_facet | Ambarish Vijayaraghava Radhika K |
| author_sort | Ambarish Vijayaraghava |
| collection | DOAJ |
| description | Introduction: Practice of regular exercise is beneficial for health.
Physical exercises have been demonstrated to alter levels of
the cytokine interferon Gamma in plasma. IFN-γ is known to be
an anti-inflammatory cytokine.
Materials and Methods: We assessed the effect of single bout
of moderate exercise and a single bout of strenuous exercise
and one month of regular moderate exercise on plasma IFN-γ.
The study consisted of 18 healthy volunteers (10 males and 8
females) with the mean age, 20.94 years, range, 18-25 years.
The exercise regime adopted is the standardized 10m Shuttle
Walk Test regime. IFN-γ was estimated using the Sandwich
ELISA technique. The reagent kit was obtained from Duoset
ELISA Development System of R & D Systems Europe Ltd. The
readings were taken at 450nm using Organon Teknika Reader
230S. Statistical methods: Friedman test has been used for
analysing IFN-γ values.
Results: Mean and SD values of IFN-γ (in picograms per ml) for
baseline (no exercise) was: 54.56 ± 28.54 (log transformation:
1.68±0.23), for acute moderate exercise: 28.94 ± 38.46 (log
transformation: 1.34 ± 0.24), for acute strenuous exercise: 20.06
± 16.96 (log transformation: 1.18 ±0.33) and after one month of
regular moderate exercise: 106.33 ± 21.51 (log transformation:
2.02 ± 0.09). The change in IFN-γ levels showed significant
difference between; a) baseline and moderate exercise, b)
baseline and strenuous exercise, c) moderate and strenuous
exercise, d) strenuous exercise and end of one month of regular
moderate exercise, e) baseline and end of one month of regular
moderate exercise, f) moderate exercise and end of one month
of regular moderate exercise. IFN-γ showed overall significance
between different grades of exercise (p < 0.05).
Conclusion: Plasma IFN-γ decreases with one bout of acute
moderate exercise, it decreases further with one bout of acute
strenuous exercise and increases at end of one month of regular
moderate exercise, which is more than baseline value. This
shows that regular moderate exercise has beneficial effects on
health by way of increasing plasma IFN-γ level. |
| first_indexed | 2024-12-21T03:34:29Z |
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| id | doaj.art-09fcbc2f2f0c4cacb67e5ab88137472a |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-21T03:34:29Z |
| publishDate | 2014-06-01 |
| publisher | JCDR Research and Publications Private Limited |
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| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-09fcbc2f2f0c4cacb67e5ab88137472a2022-12-21T19:17:23ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2014-06-0186BC05BC0710.7860/JCDR/2014/9502.4440Alteration of Interferon Gamma (IFN-γ) in Human Plasma with Graded Physical ActivityAmbarish Vijayaraghava0Radhika K1Associate Professor, Department of Physiology, M.S. Ramaiah Medical College, Bangalore, India.Lecturer Cum Statistician, Department of Preventive and Social Medicine, M.S. Ramaiah Medical College, Bangalore, India.Introduction: Practice of regular exercise is beneficial for health. Physical exercises have been demonstrated to alter levels of the cytokine interferon Gamma in plasma. IFN-γ is known to be an anti-inflammatory cytokine. Materials and Methods: We assessed the effect of single bout of moderate exercise and a single bout of strenuous exercise and one month of regular moderate exercise on plasma IFN-γ. The study consisted of 18 healthy volunteers (10 males and 8 females) with the mean age, 20.94 years, range, 18-25 years. The exercise regime adopted is the standardized 10m Shuttle Walk Test regime. IFN-γ was estimated using the Sandwich ELISA technique. The reagent kit was obtained from Duoset ELISA Development System of R & D Systems Europe Ltd. The readings were taken at 450nm using Organon Teknika Reader 230S. Statistical methods: Friedman test has been used for analysing IFN-γ values. Results: Mean and SD values of IFN-γ (in picograms per ml) for baseline (no exercise) was: 54.56 ± 28.54 (log transformation: 1.68±0.23), for acute moderate exercise: 28.94 ± 38.46 (log transformation: 1.34 ± 0.24), for acute strenuous exercise: 20.06 ± 16.96 (log transformation: 1.18 ±0.33) and after one month of regular moderate exercise: 106.33 ± 21.51 (log transformation: 2.02 ± 0.09). The change in IFN-γ levels showed significant difference between; a) baseline and moderate exercise, b) baseline and strenuous exercise, c) moderate and strenuous exercise, d) strenuous exercise and end of one month of regular moderate exercise, e) baseline and end of one month of regular moderate exercise, f) moderate exercise and end of one month of regular moderate exercise. IFN-γ showed overall significance between different grades of exercise (p < 0.05). Conclusion: Plasma IFN-γ decreases with one bout of acute moderate exercise, it decreases further with one bout of acute strenuous exercise and increases at end of one month of regular moderate exercise, which is more than baseline value. This shows that regular moderate exercise has beneficial effects on health by way of increasing plasma IFN-γ level.https://jcdr.net/articles/PDF/4440/9502_CE(Ra1)_F(H)_PF1(PAK)_PFA(AK)_PF2(AGAK).pdfinterferon gammaexerciseinflammation |
| spellingShingle | Ambarish Vijayaraghava Radhika K Alteration of Interferon Gamma (IFN-γ) in Human Plasma with Graded Physical Activity Journal of Clinical and Diagnostic Research interferon gamma exercise inflammation |
| title | Alteration of Interferon Gamma (IFN-γ) in Human Plasma with Graded Physical Activity |
| title_full | Alteration of Interferon Gamma (IFN-γ) in Human Plasma with Graded Physical Activity |
| title_fullStr | Alteration of Interferon Gamma (IFN-γ) in Human Plasma with Graded Physical Activity |
| title_full_unstemmed | Alteration of Interferon Gamma (IFN-γ) in Human Plasma with Graded Physical Activity |
| title_short | Alteration of Interferon Gamma (IFN-γ) in Human Plasma with Graded Physical Activity |
| title_sort | alteration of interferon gamma ifn γ in human plasma with graded physical activity |
| topic | interferon gamma exercise inflammation |
| url | https://jcdr.net/articles/PDF/4440/9502_CE(Ra1)_F(H)_PF1(PAK)_PFA(AK)_PF2(AGAK).pdf |
| work_keys_str_mv | AT ambarishvijayaraghava alterationofinterferongammaifnginhumanplasmawithgradedphysicalactivity AT radhikak alterationofinterferongammaifnginhumanplasmawithgradedphysicalactivity |