Rapid diagnosis of bovine brucellosis by peptide nucleic acid-fluorescence in situ hybridization assay
Brucellosis is a major economical disease of livestock and an important zoonotic disease. Rapid and accurate diagnosis is a fundamental prerequisite in the quest for control and elimination of brucellosis. The current study was conducted to evaluate a rapid molecular technique, peptide nucleic acid...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2022-08-01
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| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/106906 |
| _version_ | 1828039063605608448 |
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| author | K S LAKSHMIKANTH N S SHARMA PAVITER KAUR D PATHAK A K ARORA |
| author_facet | K S LAKSHMIKANTH N S SHARMA PAVITER KAUR D PATHAK A K ARORA |
| author_sort | K S LAKSHMIKANTH |
| collection | DOAJ |
| description |
Brucellosis is a major economical disease of livestock and an important zoonotic disease. Rapid and accurate diagnosis is a fundamental prerequisite in the quest for control and elimination of brucellosis. The current study was conducted to evaluate a rapid molecular technique, peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) assay for the diagnosis of brucellosis. Samples, including fetal stomach contents, vaginal discharges, and placenta collected from cattle and buffaloes experiencing abortion and other reproductive disorders were processed for isolation. Brucella isolates obtained were confirmed by biochemical tests and polymerase chain reaction (PCR), thereby subjected to PNA-FISH which yielded positive fluorescence. The clinical samples were processed and subjected directly to PNA-FISH. On performing PNA-FISH directly on the clinical samples, this test reduces the time spent on processing the sample for isolation, avoiding the risk of handling the infectious organism while going through the process of isolation. Hence, PNA FISH can be considered as a rapid and efficient diagnostic method for Brucellosis.
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| first_indexed | 2024-04-10T16:36:20Z |
| format | Article |
| id | doaj.art-09fdeabb2d72490986e5bcbdab7a61a5 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-10T16:36:20Z |
| publishDate | 2022-08-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-09fdeabb2d72490986e5bcbdab7a61a52023-02-08T11:23:12ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272022-08-0192810.56093/ijans.v92i8.106906Rapid diagnosis of bovine brucellosis by peptide nucleic acid-fluorescence in situ hybridization assayK S LAKSHMIKANTH0N S SHARMA1PAVITER KAUR2D PATHAK3A K ARORA4Guru Angad Dev Veterinary and Animal Sciences University, LudhianaGuru Angad Dev Veterinary and Animal Sciences University, LudhianaGuru Angad Dev Veterinary and Animal Sciences University, LudhianaGuru Angad Dev Veterinary and Animal Sciences University, LudhianaGuru Angad Dev Veterinary and Animal Sciences University, Ludhiana Brucellosis is a major economical disease of livestock and an important zoonotic disease. Rapid and accurate diagnosis is a fundamental prerequisite in the quest for control and elimination of brucellosis. The current study was conducted to evaluate a rapid molecular technique, peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) assay for the diagnosis of brucellosis. Samples, including fetal stomach contents, vaginal discharges, and placenta collected from cattle and buffaloes experiencing abortion and other reproductive disorders were processed for isolation. Brucella isolates obtained were confirmed by biochemical tests and polymerase chain reaction (PCR), thereby subjected to PNA-FISH which yielded positive fluorescence. The clinical samples were processed and subjected directly to PNA-FISH. On performing PNA-FISH directly on the clinical samples, this test reduces the time spent on processing the sample for isolation, avoiding the risk of handling the infectious organism while going through the process of isolation. Hence, PNA FISH can be considered as a rapid and efficient diagnostic method for Brucellosis. https://epubs.icar.org.in/index.php/IJAnS/article/view/106906Brucella, Hybridization, Isolation, PCR, PNA-FISH |
| spellingShingle | K S LAKSHMIKANTH N S SHARMA PAVITER KAUR D PATHAK A K ARORA Rapid diagnosis of bovine brucellosis by peptide nucleic acid-fluorescence in situ hybridization assay Indian Journal of Animal Sciences Brucella, Hybridization, Isolation, PCR, PNA-FISH |
| title | Rapid diagnosis of bovine brucellosis by peptide nucleic acid-fluorescence in situ hybridization assay |
| title_full | Rapid diagnosis of bovine brucellosis by peptide nucleic acid-fluorescence in situ hybridization assay |
| title_fullStr | Rapid diagnosis of bovine brucellosis by peptide nucleic acid-fluorescence in situ hybridization assay |
| title_full_unstemmed | Rapid diagnosis of bovine brucellosis by peptide nucleic acid-fluorescence in situ hybridization assay |
| title_short | Rapid diagnosis of bovine brucellosis by peptide nucleic acid-fluorescence in situ hybridization assay |
| title_sort | rapid diagnosis of bovine brucellosis by peptide nucleic acid fluorescence in situ hybridization assay |
| topic | Brucella, Hybridization, Isolation, PCR, PNA-FISH |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/106906 |
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