The Improved Method for Determination of Orotic Acid in Milk by Ultra-Fast Liquid Chromatography with Optimized Photodiode Array Detection

The Improved Method for Determination of Orotic Acid in Milk by Ultra-Fast Liquid Chromatography with Optimized Photodiode Array Detection

Ultra-fast liquid chromatography (UFLC) with a photodiode array detector (DAD) for simple and rapid determination of orotic acid (OAc) in milk of sheep and cows is described. Milk samples are treated with acetonitrile (1:1, <i>v/v</i>) and then centrifuged at 4 °C. To 1 mL of the obtaine...

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Bibliographic Details
Main Authors:	Marian Czauderna, Małgorzata Białek, Edyta Molik, Kamil Zaworski
Format:	Article
Language:	English
Published:	MDPI AG 2021-11-01
Series:	Animals
Subjects:	orotic acid sheep cow reversed-phase chromatography
Online Access:	https://www.mdpi.com/2076-2615/11/11/3196

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