Material Exchange in Photoreceptor Transplantation: Updating Our Understanding of Donor/Host Communication and the Future of Cell Engraftment Science
Considerable research effort has been invested into the transplantation of mammalian photoreceptors into healthy and degenerating mouse eyes. Several platforms of rod and cone fluorescent reporting have been central to refining the isolation, purification and transplantation of photoreceptors. The t...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-03-01
|
| Series: | Frontiers in Neural Circuits |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fncir.2018.00017/full |
| _version_ | 1818295595702419456 |
|---|---|
| author | Philip E. B. Nickerson Arturo Ortin-Martinez Valerie A. Wallace Valerie A. Wallace Valerie A. Wallace |
| author_facet | Philip E. B. Nickerson Arturo Ortin-Martinez Valerie A. Wallace Valerie A. Wallace Valerie A. Wallace |
| author_sort | Philip E. B. Nickerson |
| collection | DOAJ |
| description | Considerable research effort has been invested into the transplantation of mammalian photoreceptors into healthy and degenerating mouse eyes. Several platforms of rod and cone fluorescent reporting have been central to refining the isolation, purification and transplantation of photoreceptors. The tracking of engrafted cells, including identifying the position, morphology and degree of donor cell integration post-transplant is highly dependent on the use of fluorescent protein reporters. Improvements in imaging and analysis of transplant recipients have revealed that donor cell fluorescent reporters can transfer into host tissue though a process termed material exchange (ME). This recent discovery has chaperoned a new era of interpretation when reviewing the field’s use of dissociated donor cell preparations, and has prompted scientists to re-examine how we use and interpret the information derived from fluorescence-based tracking tools. In this review, we describe the status of our understanding of ME in photoreceptor transplantation. In addition, we discuss the impact of this discovery on several aspects of historical rod and cone transplantation data, and provide insight into future standards and approaches to advance the field of cell engraftment. |
| first_indexed | 2024-12-13T03:50:09Z |
| format | Article |
| id | doaj.art-0a0347c392af444a8a202bfb2f64a3da |
| institution | Directory Open Access Journal |
| issn | 1662-5110 |
| language | English |
| last_indexed | 2024-12-13T03:50:09Z |
| publishDate | 2018-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neural Circuits |
| spelling | doaj.art-0a0347c392af444a8a202bfb2f64a3da2022-12-22T00:00:43ZengFrontiers Media S.A.Frontiers in Neural Circuits1662-51102018-03-011210.3389/fncir.2018.00017316421Material Exchange in Photoreceptor Transplantation: Updating Our Understanding of Donor/Host Communication and the Future of Cell Engraftment SciencePhilip E. B. Nickerson0Arturo Ortin-Martinez1Valerie A. Wallace2Valerie A. Wallace3Valerie A. Wallace4Donald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, Toronto, ON, CanadaDonald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, Toronto, ON, CanadaDonald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, Toronto, ON, CanadaDepartment of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, CanadaDepartment of Ophthalmology and Vision Sciences, University of Toronto, Toronto, ON, CanadaConsiderable research effort has been invested into the transplantation of mammalian photoreceptors into healthy and degenerating mouse eyes. Several platforms of rod and cone fluorescent reporting have been central to refining the isolation, purification and transplantation of photoreceptors. The tracking of engrafted cells, including identifying the position, morphology and degree of donor cell integration post-transplant is highly dependent on the use of fluorescent protein reporters. Improvements in imaging and analysis of transplant recipients have revealed that donor cell fluorescent reporters can transfer into host tissue though a process termed material exchange (ME). This recent discovery has chaperoned a new era of interpretation when reviewing the field’s use of dissociated donor cell preparations, and has prompted scientists to re-examine how we use and interpret the information derived from fluorescence-based tracking tools. In this review, we describe the status of our understanding of ME in photoreceptor transplantation. In addition, we discuss the impact of this discovery on several aspects of historical rod and cone transplantation data, and provide insight into future standards and approaches to advance the field of cell engraftment.http://journal.frontiersin.org/article/10.3389/fncir.2018.00017/fullphotoreceptorstransplantationmaterial exchangeretinal degeneration |
| spellingShingle | Philip E. B. Nickerson Arturo Ortin-Martinez Valerie A. Wallace Valerie A. Wallace Valerie A. Wallace Material Exchange in Photoreceptor Transplantation: Updating Our Understanding of Donor/Host Communication and the Future of Cell Engraftment Science Frontiers in Neural Circuits photoreceptors transplantation material exchange retinal degeneration |
| title | Material Exchange in Photoreceptor Transplantation: Updating Our Understanding of Donor/Host Communication and the Future of Cell Engraftment Science |
| title_full | Material Exchange in Photoreceptor Transplantation: Updating Our Understanding of Donor/Host Communication and the Future of Cell Engraftment Science |
| title_fullStr | Material Exchange in Photoreceptor Transplantation: Updating Our Understanding of Donor/Host Communication and the Future of Cell Engraftment Science |
| title_full_unstemmed | Material Exchange in Photoreceptor Transplantation: Updating Our Understanding of Donor/Host Communication and the Future of Cell Engraftment Science |
| title_short | Material Exchange in Photoreceptor Transplantation: Updating Our Understanding of Donor/Host Communication and the Future of Cell Engraftment Science |
| title_sort | material exchange in photoreceptor transplantation updating our understanding of donor host communication and the future of cell engraftment science |
| topic | photoreceptors transplantation material exchange retinal degeneration |
| url | http://journal.frontiersin.org/article/10.3389/fncir.2018.00017/full |
| work_keys_str_mv | AT philipebnickerson materialexchangeinphotoreceptortransplantationupdatingourunderstandingofdonorhostcommunicationandthefutureofcellengraftmentscience AT arturoortinmartinez materialexchangeinphotoreceptortransplantationupdatingourunderstandingofdonorhostcommunicationandthefutureofcellengraftmentscience AT valerieawallace materialexchangeinphotoreceptortransplantationupdatingourunderstandingofdonorhostcommunicationandthefutureofcellengraftmentscience AT valerieawallace materialexchangeinphotoreceptortransplantationupdatingourunderstandingofdonorhostcommunicationandthefutureofcellengraftmentscience AT valerieawallace materialexchangeinphotoreceptortransplantationupdatingourunderstandingofdonorhostcommunicationandthefutureofcellengraftmentscience |