Surface Properties of a Biocompatible Thermoplastic Polyurethane and Its Anti-Adhesive Effect against <i>E. coli</i> and <i>S. aureus</i>
Thermoplastic polyurethane (TPU) is a polymer used in a variety of fields, including medical applications. Here, we aimed to verify if the brush and bar coater deposition techniques did not alter TPU properties. The topography of the TPU-modified surfaces was studied via AFM demonstrating no signifi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/15/1/24 |
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| author | Elisa Restivo Emanuela Peluso Nora Bloise Giovanni Lo Bello Giovanna Bruni Marialaura Giannaccari Roberto Raiteri Lorenzo Fassina Livia Visai |
| author_facet | Elisa Restivo Emanuela Peluso Nora Bloise Giovanni Lo Bello Giovanna Bruni Marialaura Giannaccari Roberto Raiteri Lorenzo Fassina Livia Visai |
| author_sort | Elisa Restivo |
| collection | DOAJ |
| description | Thermoplastic polyurethane (TPU) is a polymer used in a variety of fields, including medical applications. Here, we aimed to verify if the brush and bar coater deposition techniques did not alter TPU properties. The topography of the TPU-modified surfaces was studied via AFM demonstrating no significant differences between brush and bar coater-modified surfaces, compared to the un-modified TPU (TPU Film). The effect of the surfaces on planktonic bacteria, evaluated by MTT assay, demonstrated their anti-adhesive effect on <i>E. coli</i>, while the bar coater significantly reduced staphylococcal planktonic adhesion and both bacterial biofilms compared to other samples. Interestingly, Pearson’s R coefficient analysis showed that <i>R</i><sub>a</sub> roughness and Haralick’s correlation feature were trend predictors for planktonic bacterial cells adhesion. The surface adhesion property was evaluated against NIH-3T3 murine fibroblasts by MTT and against human fibrinogen and human platelet-rich plasma by ELISA and LDH assay, respectively. An indirect cytotoxicity experiment against NIH-3T3 confirmed the biocompatibility of the TPUs. Overall, the results indicated that the deposition techniques did not alter the antibacterial and anti-adhesive surface properties of modified TPU compared to un-modified TPU, nor its bio- and hemocompatibility, confirming the suitability of TPU brush and bar coater films in the biomedical and pharmaceutical fields. |
| first_indexed | 2024-03-08T10:45:55Z |
| format | Article |
| id | doaj.art-0a0d48859b9644a7bafee3f71179e024 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-08T10:45:55Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-0a0d48859b9644a7bafee3f71179e0242024-01-26T17:10:58ZengMDPI AGJournal of Functional Biomaterials2079-49832024-01-011512410.3390/jfb15010024Surface Properties of a Biocompatible Thermoplastic Polyurethane and Its Anti-Adhesive Effect against <i>E. coli</i> and <i>S. aureus</i>Elisa Restivo0Emanuela Peluso1Nora Bloise2Giovanni Lo Bello3Giovanna Bruni4Marialaura Giannaccari5Roberto Raiteri6Lorenzo Fassina7Livia Visai8Department of Molecular Medicine, Centre for Health Technologies (CHT), Consorzio Interuniversitario Nazionale per la Scienza e la Tecnologia dei Materiali (INSTM), Research Unit (UdR) Pavia, University of Pavia, 27100 Pavia, ItalyDepartment of Molecular Medicine, Centre for Health Technologies (CHT), Consorzio Interuniversitario Nazionale per la Scienza e la Tecnologia dei Materiali (INSTM), Research Unit (UdR) Pavia, University of Pavia, 27100 Pavia, ItalyDepartment of Molecular Medicine, Centre for Health Technologies (CHT), Consorzio Interuniversitario Nazionale per la Scienza e la Tecnologia dei Materiali (INSTM), Research Unit (UdR) Pavia, University of Pavia, 27100 Pavia, ItalyDepartment of Informatics, Bioengineering, Robotics and System Engineering—DIBRIS, University of Genoa, 16145 Genoa, ItalyDepartment of Chemistry, Physical Chemistry Section, University of Pavia, 27100 Pavia, ItalyDepartment of Molecular Medicine, Centre for Health Technologies (CHT), Consorzio Interuniversitario Nazionale per la Scienza e la Tecnologia dei Materiali (INSTM), Research Unit (UdR) Pavia, University of Pavia, 27100 Pavia, ItalyDepartment of Informatics, Bioengineering, Robotics and System Engineering—DIBRIS, University of Genoa, 16145 Genoa, ItalyDepartment of Electrical, Computer and Biomedical Engineering, Centre for Health Technologies (CHT), University of Pavia, 27100 Pavia, ItalyDepartment of Molecular Medicine, Centre for Health Technologies (CHT), Consorzio Interuniversitario Nazionale per la Scienza e la Tecnologia dei Materiali (INSTM), Research Unit (UdR) Pavia, University of Pavia, 27100 Pavia, ItalyThermoplastic polyurethane (TPU) is a polymer used in a variety of fields, including medical applications. Here, we aimed to verify if the brush and bar coater deposition techniques did not alter TPU properties. The topography of the TPU-modified surfaces was studied via AFM demonstrating no significant differences between brush and bar coater-modified surfaces, compared to the un-modified TPU (TPU Film). The effect of the surfaces on planktonic bacteria, evaluated by MTT assay, demonstrated their anti-adhesive effect on <i>E. coli</i>, while the bar coater significantly reduced staphylococcal planktonic adhesion and both bacterial biofilms compared to other samples. Interestingly, Pearson’s R coefficient analysis showed that <i>R</i><sub>a</sub> roughness and Haralick’s correlation feature were trend predictors for planktonic bacterial cells adhesion. The surface adhesion property was evaluated against NIH-3T3 murine fibroblasts by MTT and against human fibrinogen and human platelet-rich plasma by ELISA and LDH assay, respectively. An indirect cytotoxicity experiment against NIH-3T3 confirmed the biocompatibility of the TPUs. Overall, the results indicated that the deposition techniques did not alter the antibacterial and anti-adhesive surface properties of modified TPU compared to un-modified TPU, nor its bio- and hemocompatibility, confirming the suitability of TPU brush and bar coater films in the biomedical and pharmaceutical fields.https://www.mdpi.com/2079-4983/15/1/24thermoplastic polyurethane (TPU)brushbar coatertopographyatomic force microscopy (AFM)Haralick texture analysis |
| spellingShingle | Elisa Restivo Emanuela Peluso Nora Bloise Giovanni Lo Bello Giovanna Bruni Marialaura Giannaccari Roberto Raiteri Lorenzo Fassina Livia Visai Surface Properties of a Biocompatible Thermoplastic Polyurethane and Its Anti-Adhesive Effect against <i>E. coli</i> and <i>S. aureus</i> Journal of Functional Biomaterials thermoplastic polyurethane (TPU) brush bar coater topography atomic force microscopy (AFM) Haralick texture analysis |
| title | Surface Properties of a Biocompatible Thermoplastic Polyurethane and Its Anti-Adhesive Effect against <i>E. coli</i> and <i>S. aureus</i> |
| title_full | Surface Properties of a Biocompatible Thermoplastic Polyurethane and Its Anti-Adhesive Effect against <i>E. coli</i> and <i>S. aureus</i> |
| title_fullStr | Surface Properties of a Biocompatible Thermoplastic Polyurethane and Its Anti-Adhesive Effect against <i>E. coli</i> and <i>S. aureus</i> |
| title_full_unstemmed | Surface Properties of a Biocompatible Thermoplastic Polyurethane and Its Anti-Adhesive Effect against <i>E. coli</i> and <i>S. aureus</i> |
| title_short | Surface Properties of a Biocompatible Thermoplastic Polyurethane and Its Anti-Adhesive Effect against <i>E. coli</i> and <i>S. aureus</i> |
| title_sort | surface properties of a biocompatible thermoplastic polyurethane and its anti adhesive effect against i e coli i and i s aureus i |
| topic | thermoplastic polyurethane (TPU) brush bar coater topography atomic force microscopy (AFM) Haralick texture analysis |
| url | https://www.mdpi.com/2079-4983/15/1/24 |
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