GENETIC POLYMORPHISM AND CYTOGENETIC CHANGES IN THE PERIPHERAL BLOOD T-LYMPHOCYTES OF PATIENTS WITH ARTHRITIS ASSOCIATED WITH IXODES TICK-BORNE BORRELIOSIS IN THE NORTHERN REGIONS OF SIBERIA
The authors have previously conducted studies that demonstrate the increased level of cytogenetic disturbances in patients with Ixodes tick-borne borreliosis (ITB). The severity of arthritis associated with ITB (AITB) is also ascertained to depend on whether the patient has certain HLA-DRB1 alleles....
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| Format: | Article |
| Language: | Russian |
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IMA PRESS LLC
2017-10-01
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| Series: | Научно-практическая ревматология |
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| Online Access: | https://rsp.mediar-press.net/rsp/article/view/2431 |
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| author | N. N. Ilyinskikh E. N. Ilyinskikh V. P. Zuevsky A. G. Semenov |
| author_facet | N. N. Ilyinskikh E. N. Ilyinskikh V. P. Zuevsky A. G. Semenov |
| author_sort | N. N. Ilyinskikh |
| collection | DOAJ |
| description | The authors have previously conducted studies that demonstrate the increased level of cytogenetic disturbances in patients with Ixodes tick-borne borreliosis (ITB). The severity of arthritis associated with ITB (AITB) is also ascertained to depend on whether the patient has certain HLA-DRB1 alleles.Objective: to assess the association between HLA-DRB1 gene polymorphism and cytogenetic changes in the peripheral blood T lymphocytes of patients with AITB.Subjects and methods. 146 patients with AITB, 100 clinically healthy convalescents with ITB (CITB), and a control group of 98 healthy blood donors (HBDs) without a history of tick-borne infections were examined using cytogenetic (micronucleus analysis of cytokinesis-blocked peripheral blood T lymphocytes) and molecular genetic (PCR analysis of HLA-DRB1 gene polymorphism) methods.Results and discussion. The frequency of cytokinesis-blocked lymphocytes with micronuclei in the AITB group was significantly higher than that in the CITB and HBD groups (p<0.01) with the exception of the results obtained in the subgroup of patients with AITB who had the DRB1*10 allele (p>0.05). The highest levels of lymphocytes with micronuclei were observed in AITB patients with the DRB1*17(03), *01, and *04 alleles as compared to those in the CITB and HBD groups (p<0.001). The CITB group showed the most significant increase in the detection rate of lymphocytes with micronuclei in people with the DRB1*01, DRB1*04, or *17(03) alleles. At the same time, there were no significant differences in the number of lymphocytes with micronuclei in the HBD group, depending on the HLA-DRB1 gene alleles (p>0.05). Thus, the patients with AITB had the highest frequency of cytogenetic disorders with the exception of individuals with the DRB1*10 allele. |
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| issn | 1995-4484 1995-4492 |
| language | Russian |
| last_indexed | 2024-04-09T22:23:37Z |
| publishDate | 2017-10-01 |
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| series | Научно-практическая ревматология |
| spelling | doaj.art-0a0dc86f1f8048f89e49d418ee5d8ca72023-03-22T13:45:53ZrusIMA PRESS LLCНаучно-практическая ревматология1995-44841995-44922017-10-0155550050310.14412/1995-4484-2017-500-5032267GENETIC POLYMORPHISM AND CYTOGENETIC CHANGES IN THE PERIPHERAL BLOOD T-LYMPHOCYTES OF PATIENTS WITH ARTHRITIS ASSOCIATED WITH IXODES TICK-BORNE BORRELIOSIS IN THE NORTHERN REGIONS OF SIBERIAN. N. Ilyinskikh0E. N. Ilyinskikh1V. P. Zuevsky2A. G. Semenov3Siberian State Medical University, Ministry of Health of Russia National Research Tomsk State University, Ministry of Education and Science of Russia Tomsk State Pedagogical University, Ministry of Education and Science of RussiaSiberian State Medical University, Ministry of Health of Russia National Research Tomsk State University, Ministry of Education and Science of RussiaKhanty-Mansi State Medical Academy, Ministry of Education and Science of Russia, Khanty- Mansi Autonomous District-YugrSiberian State Medical University, Ministry of Health of RussiaThe authors have previously conducted studies that demonstrate the increased level of cytogenetic disturbances in patients with Ixodes tick-borne borreliosis (ITB). The severity of arthritis associated with ITB (AITB) is also ascertained to depend on whether the patient has certain HLA-DRB1 alleles.Objective: to assess the association between HLA-DRB1 gene polymorphism and cytogenetic changes in the peripheral blood T lymphocytes of patients with AITB.Subjects and methods. 146 patients with AITB, 100 clinically healthy convalescents with ITB (CITB), and a control group of 98 healthy blood donors (HBDs) without a history of tick-borne infections were examined using cytogenetic (micronucleus analysis of cytokinesis-blocked peripheral blood T lymphocytes) and molecular genetic (PCR analysis of HLA-DRB1 gene polymorphism) methods.Results and discussion. The frequency of cytokinesis-blocked lymphocytes with micronuclei in the AITB group was significantly higher than that in the CITB and HBD groups (p<0.01) with the exception of the results obtained in the subgroup of patients with AITB who had the DRB1*10 allele (p>0.05). The highest levels of lymphocytes with micronuclei were observed in AITB patients with the DRB1*17(03), *01, and *04 alleles as compared to those in the CITB and HBD groups (p<0.001). The CITB group showed the most significant increase in the detection rate of lymphocytes with micronuclei in people with the DRB1*01, DRB1*04, or *17(03) alleles. At the same time, there were no significant differences in the number of lymphocytes with micronuclei in the HBD group, depending on the HLA-DRB1 gene alleles (p>0.05). Thus, the patients with AITB had the highest frequency of cytogenetic disorders with the exception of individuals with the DRB1*10 allele.https://rsp.mediar-press.net/rsp/article/view/2431ixodes tick-borne borreliosishla-drb1genetic polymorphismt lymphocytesmicronucleus analysis |
| spellingShingle | N. N. Ilyinskikh E. N. Ilyinskikh V. P. Zuevsky A. G. Semenov GENETIC POLYMORPHISM AND CYTOGENETIC CHANGES IN THE PERIPHERAL BLOOD T-LYMPHOCYTES OF PATIENTS WITH ARTHRITIS ASSOCIATED WITH IXODES TICK-BORNE BORRELIOSIS IN THE NORTHERN REGIONS OF SIBERIA Научно-практическая ревматология ixodes tick-borne borreliosis hla-drb1 genetic polymorphism t lymphocytes micronucleus analysis |
| title | GENETIC POLYMORPHISM AND CYTOGENETIC CHANGES IN THE PERIPHERAL BLOOD T-LYMPHOCYTES OF PATIENTS WITH ARTHRITIS ASSOCIATED WITH IXODES TICK-BORNE BORRELIOSIS IN THE NORTHERN REGIONS OF SIBERIA |
| title_full | GENETIC POLYMORPHISM AND CYTOGENETIC CHANGES IN THE PERIPHERAL BLOOD T-LYMPHOCYTES OF PATIENTS WITH ARTHRITIS ASSOCIATED WITH IXODES TICK-BORNE BORRELIOSIS IN THE NORTHERN REGIONS OF SIBERIA |
| title_fullStr | GENETIC POLYMORPHISM AND CYTOGENETIC CHANGES IN THE PERIPHERAL BLOOD T-LYMPHOCYTES OF PATIENTS WITH ARTHRITIS ASSOCIATED WITH IXODES TICK-BORNE BORRELIOSIS IN THE NORTHERN REGIONS OF SIBERIA |
| title_full_unstemmed | GENETIC POLYMORPHISM AND CYTOGENETIC CHANGES IN THE PERIPHERAL BLOOD T-LYMPHOCYTES OF PATIENTS WITH ARTHRITIS ASSOCIATED WITH IXODES TICK-BORNE BORRELIOSIS IN THE NORTHERN REGIONS OF SIBERIA |
| title_short | GENETIC POLYMORPHISM AND CYTOGENETIC CHANGES IN THE PERIPHERAL BLOOD T-LYMPHOCYTES OF PATIENTS WITH ARTHRITIS ASSOCIATED WITH IXODES TICK-BORNE BORRELIOSIS IN THE NORTHERN REGIONS OF SIBERIA |
| title_sort | genetic polymorphism and cytogenetic changes in the peripheral blood t lymphocytes of patients with arthritis associated with ixodes tick borne borreliosis in the northern regions of siberia |
| topic | ixodes tick-borne borreliosis hla-drb1 genetic polymorphism t lymphocytes micronucleus analysis |
| url | https://rsp.mediar-press.net/rsp/article/view/2431 |
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