Physiological and Transcriptional Analyses Provide Insight into Maintaining Ion Homeostasis of Sweet Sorghum under Salt Stress
Sweet sorghum is an important bioenergy grass and valuable forage with a strong adaptability to saline environments. However, little is known about the mechanisms of sweet sorghum coping with ion toxicity under salt stresses. Here, we first evaluated the salt tolerance of a sweet sorghum cultivar “L...
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MDPI AG
2023-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/13/11045 |
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| author | Huan Guo Chun-Ya Nie Zhen Li Jie Kang Xiao-Long Wang Yan-Nong Cui |
| author_facet | Huan Guo Chun-Ya Nie Zhen Li Jie Kang Xiao-Long Wang Yan-Nong Cui |
| author_sort | Huan Guo |
| collection | DOAJ |
| description | Sweet sorghum is an important bioenergy grass and valuable forage with a strong adaptability to saline environments. However, little is known about the mechanisms of sweet sorghum coping with ion toxicity under salt stresses. Here, we first evaluated the salt tolerance of a sweet sorghum cultivar “Lvjuren” and determined its ion accumulation traits under NaCl treatments; then, we explored key genes involved in Na<sup>+</sup>, Cl<sup>−</sup>, K<sup>+</sup> and NO<sub>3</sub><sup>−</sup> transport using transcriptome profiling and the qRT-PCR method. The results showed that growth and photosynthesis of sweet sorghum were unaffected by 50 and 100 mM NaCl treatments, indicative of a strong salt tolerance of this species. Under NaCl treatments, sweet sorghum could efficiently exclude Na<sup>+</sup> from shoots and accumulate Cl<sup>−</sup> in leaf sheaths to avoid their overaccumulation in leaf blades; meanwhile, it possessed a prominent ability to sustain NO<sub>3</sub><sup>−</sup> homeostasis in leaf blades. Transcriptome profiling identified several differentially expressed genes associated with Na<sup>+</sup>, Cl<sup>−</sup>, K<sup>+</sup> and NO<sub>3</sub><sup>−</sup> transport in roots, leaf sheaths and leaf blades after 200 mM NaCl treatment for 6 and 48 h. Moreover, transcriptome data and qRT-PCR results indicated that <i>HKT1;5</i>, <i>CLCc</i> and <i>NPF7.3-1</i> should be key genes involved in Na<sup>+</sup> retention in roots, Cl<sup>−</sup> accumulation in leaf sheaths and maintenance of NO<sub>3</sub><sup>−</sup> homeostasis in leaf blades, respectively. Many <i>TFs</i> were also identified after NaCl treatment, which should play important regulatory roles in salt tolerance of sweet sorghum. In addition, GO analysis identified candidate genes involved in maintaining membrane stability and photosynthetic capacity under salt stresses. This work lays a preliminary foundation for clarifying the molecular basis underlying the adaptation of sweet sorghum to adverse environments. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T01:37:44Z |
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| spelling | doaj.art-0a0ed17b495f4171a4368622a2e096972023-11-18T16:47:20ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-07-0124131104510.3390/ijms241311045Physiological and Transcriptional Analyses Provide Insight into Maintaining Ion Homeostasis of Sweet Sorghum under Salt StressHuan Guo0Chun-Ya Nie1Zhen Li2Jie Kang3Xiao-Long Wang4Yan-Nong Cui5College of Grassland Agriculture, Northwest A&F University, Yangling 712100, ChinaCollege of Grassland Agriculture, Northwest A&F University, Yangling 712100, ChinaCollege of Grassland Agriculture, Northwest A&F University, Yangling 712100, ChinaCollege of Grassland Agriculture, Northwest A&F University, Yangling 712100, ChinaCollege of Grassland Agriculture, Northwest A&F University, Yangling 712100, ChinaCollege of Grassland Agriculture, Northwest A&F University, Yangling 712100, ChinaSweet sorghum is an important bioenergy grass and valuable forage with a strong adaptability to saline environments. However, little is known about the mechanisms of sweet sorghum coping with ion toxicity under salt stresses. Here, we first evaluated the salt tolerance of a sweet sorghum cultivar “Lvjuren” and determined its ion accumulation traits under NaCl treatments; then, we explored key genes involved in Na<sup>+</sup>, Cl<sup>−</sup>, K<sup>+</sup> and NO<sub>3</sub><sup>−</sup> transport using transcriptome profiling and the qRT-PCR method. The results showed that growth and photosynthesis of sweet sorghum were unaffected by 50 and 100 mM NaCl treatments, indicative of a strong salt tolerance of this species. Under NaCl treatments, sweet sorghum could efficiently exclude Na<sup>+</sup> from shoots and accumulate Cl<sup>−</sup> in leaf sheaths to avoid their overaccumulation in leaf blades; meanwhile, it possessed a prominent ability to sustain NO<sub>3</sub><sup>−</sup> homeostasis in leaf blades. Transcriptome profiling identified several differentially expressed genes associated with Na<sup>+</sup>, Cl<sup>−</sup>, K<sup>+</sup> and NO<sub>3</sub><sup>−</sup> transport in roots, leaf sheaths and leaf blades after 200 mM NaCl treatment for 6 and 48 h. Moreover, transcriptome data and qRT-PCR results indicated that <i>HKT1;5</i>, <i>CLCc</i> and <i>NPF7.3-1</i> should be key genes involved in Na<sup>+</sup> retention in roots, Cl<sup>−</sup> accumulation in leaf sheaths and maintenance of NO<sub>3</sub><sup>−</sup> homeostasis in leaf blades, respectively. Many <i>TFs</i> were also identified after NaCl treatment, which should play important regulatory roles in salt tolerance of sweet sorghum. In addition, GO analysis identified candidate genes involved in maintaining membrane stability and photosynthetic capacity under salt stresses. This work lays a preliminary foundation for clarifying the molecular basis underlying the adaptation of sweet sorghum to adverse environments.https://www.mdpi.com/1422-0067/24/13/11045soil salinitysodiumchlorideion transporterstranscription factors |
| spellingShingle | Huan Guo Chun-Ya Nie Zhen Li Jie Kang Xiao-Long Wang Yan-Nong Cui Physiological and Transcriptional Analyses Provide Insight into Maintaining Ion Homeostasis of Sweet Sorghum under Salt Stress International Journal of Molecular Sciences soil salinity sodium chloride ion transporters transcription factors |
| title | Physiological and Transcriptional Analyses Provide Insight into Maintaining Ion Homeostasis of Sweet Sorghum under Salt Stress |
| title_full | Physiological and Transcriptional Analyses Provide Insight into Maintaining Ion Homeostasis of Sweet Sorghum under Salt Stress |
| title_fullStr | Physiological and Transcriptional Analyses Provide Insight into Maintaining Ion Homeostasis of Sweet Sorghum under Salt Stress |
| title_full_unstemmed | Physiological and Transcriptional Analyses Provide Insight into Maintaining Ion Homeostasis of Sweet Sorghum under Salt Stress |
| title_short | Physiological and Transcriptional Analyses Provide Insight into Maintaining Ion Homeostasis of Sweet Sorghum under Salt Stress |
| title_sort | physiological and transcriptional analyses provide insight into maintaining ion homeostasis of sweet sorghum under salt stress |
| topic | soil salinity sodium chloride ion transporters transcription factors |
| url | https://www.mdpi.com/1422-0067/24/13/11045 |
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