Novel pentose-regulated promoter of Aspergillus oryzae with application in controlling heterologous gene expression
The potent promoter and its transcriptional control make a significant contribution to strain optimization. Using transcriptome-based approach, a novel pentose-regulated promoter of the xylose reductase gene (PxyrA) of Aspergillus oryzae was identified. The promoter analysis showed that the PxyrA wa...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | Biotechnology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X21001119 |
| _version_ | 1819121735759298560 |
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| author | Sukanya Jeennor Jutamas Anantayanon Chanikul Chutrakul Sarocha Panchanawaporn Kobkul Laoteng |
| author_facet | Sukanya Jeennor Jutamas Anantayanon Chanikul Chutrakul Sarocha Panchanawaporn Kobkul Laoteng |
| author_sort | Sukanya Jeennor |
| collection | DOAJ |
| description | The potent promoter and its transcriptional control make a significant contribution to strain optimization. Using transcriptome-based approach, a novel pentose-regulated promoter of the xylose reductase gene (PxyrA) of Aspergillus oryzae was identified. The promoter analysis showed that the PxyrA was tightly regulated by pentose sugars, which xylose and xylan were favorable inducers. The PxyrA function was highly efficient as compared with the maltose-inducible promoters of A. oryzae. It also exhibited the efficient transcription induction even though certain amounts of glucose and sucrose existed in the cultures. The expression control of PxyrA was dependent on xylose consumption capacity for fungal growth. The control mode of PxyrA offers a simple operation in simultaneous gene expression and cultivation optimization in Aspergilli. This study provides a prospective development of fungal production platform using cellulosic sugars by the xylose-utilizing strains for sustainable growing in circular economy. |
| first_indexed | 2024-12-22T06:41:17Z |
| format | Article |
| id | doaj.art-0a0fbe3e76684f979bf25593ba45566f |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-12-22T06:41:17Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-0a0fbe3e76684f979bf25593ba45566f2022-12-21T18:35:25ZengElsevierBiotechnology Reports2215-017X2022-03-0133e00695Novel pentose-regulated promoter of Aspergillus oryzae with application in controlling heterologous gene expressionSukanya Jeennor0Jutamas Anantayanon1Chanikul Chutrakul2Sarocha Panchanawaporn3Kobkul Laoteng4Industrial Bioprocess Technology Research Team, Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani, 12120, ThailandIndustrial Bioprocess Technology Research Team, Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani, 12120, ThailandIndustrial Bioprocess Technology Research Team, Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani, 12120, ThailandIndustrial Bioprocess Technology Research Team, Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani, 12120, ThailandCorresponding author.; Industrial Bioprocess Technology Research Team, Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani, 12120, ThailandThe potent promoter and its transcriptional control make a significant contribution to strain optimization. Using transcriptome-based approach, a novel pentose-regulated promoter of the xylose reductase gene (PxyrA) of Aspergillus oryzae was identified. The promoter analysis showed that the PxyrA was tightly regulated by pentose sugars, which xylose and xylan were favorable inducers. The PxyrA function was highly efficient as compared with the maltose-inducible promoters of A. oryzae. It also exhibited the efficient transcription induction even though certain amounts of glucose and sucrose existed in the cultures. The expression control of PxyrA was dependent on xylose consumption capacity for fungal growth. The control mode of PxyrA offers a simple operation in simultaneous gene expression and cultivation optimization in Aspergilli. This study provides a prospective development of fungal production platform using cellulosic sugars by the xylose-utilizing strains for sustainable growing in circular economy.http://www.sciencedirect.com/science/article/pii/S2215017X21001119Aspergillus oryzaeGene expressionInducible promoterPentoseXylose reductase |
| spellingShingle | Sukanya Jeennor Jutamas Anantayanon Chanikul Chutrakul Sarocha Panchanawaporn Kobkul Laoteng Novel pentose-regulated promoter of Aspergillus oryzae with application in controlling heterologous gene expression Biotechnology Reports Aspergillus oryzae Gene expression Inducible promoter Pentose Xylose reductase |
| title | Novel pentose-regulated promoter of Aspergillus oryzae with application in controlling heterologous gene expression |
| title_full | Novel pentose-regulated promoter of Aspergillus oryzae with application in controlling heterologous gene expression |
| title_fullStr | Novel pentose-regulated promoter of Aspergillus oryzae with application in controlling heterologous gene expression |
| title_full_unstemmed | Novel pentose-regulated promoter of Aspergillus oryzae with application in controlling heterologous gene expression |
| title_short | Novel pentose-regulated promoter of Aspergillus oryzae with application in controlling heterologous gene expression |
| title_sort | novel pentose regulated promoter of aspergillus oryzae with application in controlling heterologous gene expression |
| topic | Aspergillus oryzae Gene expression Inducible promoter Pentose Xylose reductase |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X21001119 |
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