Antibacterial and Fluorescence Staining Properties of an Innovative GTR Membrane Containing 45S5BGs and AIE Molecules In Vitro
This study aimed to add two functional components—antibacterial 45S5BGs particles and AIE nanoparticles (TPE-NIM<sup>+</sup>) with bioprobe characteristics—to the guided tissue regeneration (GTR) membrane, to optimize the performance. The PLGA/BG/TPE-NIM<sup>+</sup> membrane...
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MDPI AG
2022-02-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/4/641 |
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| author | Yu-Wen Wei Sayed Mir Sayed Wei-Wen Zhu Ke-Fei Xu Fu-Gen Wu Jing Xu He-Peng Nie Yu-Li Wang Xiao-Lin Lu Qian Ma |
| author_facet | Yu-Wen Wei Sayed Mir Sayed Wei-Wen Zhu Ke-Fei Xu Fu-Gen Wu Jing Xu He-Peng Nie Yu-Li Wang Xiao-Lin Lu Qian Ma |
| author_sort | Yu-Wen Wei |
| collection | DOAJ |
| description | This study aimed to add two functional components—antibacterial 45S5BGs particles and AIE nanoparticles (TPE-NIM<sup>+</sup>) with bioprobe characteristics—to the guided tissue regeneration (GTR) membrane, to optimize the performance. The PLGA/BG/TPE-NIM<sup>+</sup> membrane was synthesized. The static water contact angle, morphologies, and surface element analysis of the membrane were then characterized. In vitro biocompatibility was tested with MC3T3-E1 cells using CCK-8 assay, and antibacterial property was evaluated with <i>Streptococcus mutans</i> and <i>Porphyromonas gingivalis</i> by the LIVE/DEAD bacterial staining and dilution plating procedure. The fluorescence staining of bacteria was observed by Laser Scanning Confocal Microscope. The results showed that the average water contact angle was 46°. In the cytotoxicity test, except for the positive control group, there was no significant difference among the groups (<i>p</i> > 0.05). The antibacterial effect in the PLGA/BG/TPE-NIM<sup>+</sup> group was significantly (<i>p</i> < 0.01), while the sterilization rate was 99.99%, better than that in the PLGA/BG group (98.62%) (<i>p</i> < 0.01). Confocal images showed that the membrane efficiently distinguished G<sup>+</sup> bacteria from G<sup>−</sup> bacteria. This study demonstrated that the PLGA/BG/TPE-NIM<sup>+</sup> membrane showed good biocompatibility, efficient sterilization performance, and surface mineralization ability and could be used to detect pathogens in a simple, fast, and wash-free protocol. |
| first_indexed | 2024-03-09T21:18:29Z |
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| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T21:18:29Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-0a0fe25b18e846eba531ac37be75895f2023-11-23T21:25:45ZengMDPI AGNanomaterials2079-49912022-02-0112464110.3390/nano12040641Antibacterial and Fluorescence Staining Properties of an Innovative GTR Membrane Containing 45S5BGs and AIE Molecules In VitroYu-Wen Wei0Sayed Mir Sayed1Wei-Wen Zhu2Ke-Fei Xu3Fu-Gen Wu4Jing Xu5He-Peng Nie6Yu-Li Wang7Xiao-Lin Lu8Qian Ma9Jiangsu Province Key Laboratory of Oral Diseases, Department of General Dentistry, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing 210029, ChinaState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, 2 Sipailou Road, Nanjing 210096, ChinaJiangsu Province Key Laboratory of Oral Diseases, Department of General Dentistry, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing 210029, ChinaState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, 2 Sipailou Road, Nanjing 210096, ChinaState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, 2 Sipailou Road, Nanjing 210096, ChinaJiangsu Province Key Laboratory of Oral Diseases, Department of General Dentistry, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing 210029, ChinaJiangsu Province Key Laboratory of Oral Diseases, Department of General Dentistry, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing 210029, ChinaJiangsu Province Key Laboratory of Oral Diseases, Department of General Dentistry, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing 210029, ChinaState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, 2 Sipailou Road, Nanjing 210096, ChinaJiangsu Province Key Laboratory of Oral Diseases, Department of General Dentistry, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing 210029, ChinaThis study aimed to add two functional components—antibacterial 45S5BGs particles and AIE nanoparticles (TPE-NIM<sup>+</sup>) with bioprobe characteristics—to the guided tissue regeneration (GTR) membrane, to optimize the performance. The PLGA/BG/TPE-NIM<sup>+</sup> membrane was synthesized. The static water contact angle, morphologies, and surface element analysis of the membrane were then characterized. In vitro biocompatibility was tested with MC3T3-E1 cells using CCK-8 assay, and antibacterial property was evaluated with <i>Streptococcus mutans</i> and <i>Porphyromonas gingivalis</i> by the LIVE/DEAD bacterial staining and dilution plating procedure. The fluorescence staining of bacteria was observed by Laser Scanning Confocal Microscope. The results showed that the average water contact angle was 46°. In the cytotoxicity test, except for the positive control group, there was no significant difference among the groups (<i>p</i> > 0.05). The antibacterial effect in the PLGA/BG/TPE-NIM<sup>+</sup> group was significantly (<i>p</i> < 0.01), while the sterilization rate was 99.99%, better than that in the PLGA/BG group (98.62%) (<i>p</i> < 0.01). Confocal images showed that the membrane efficiently distinguished G<sup>+</sup> bacteria from G<sup>−</sup> bacteria. This study demonstrated that the PLGA/BG/TPE-NIM<sup>+</sup> membrane showed good biocompatibility, efficient sterilization performance, and surface mineralization ability and could be used to detect pathogens in a simple, fast, and wash-free protocol.https://www.mdpi.com/2079-4991/12/4/641aggregation-induced emission nanoparticlesantibacterialantibacterial differentiationbioactive glassbioprobeguided tissue regeneration (GTR) |
| spellingShingle | Yu-Wen Wei Sayed Mir Sayed Wei-Wen Zhu Ke-Fei Xu Fu-Gen Wu Jing Xu He-Peng Nie Yu-Li Wang Xiao-Lin Lu Qian Ma Antibacterial and Fluorescence Staining Properties of an Innovative GTR Membrane Containing 45S5BGs and AIE Molecules In Vitro Nanomaterials aggregation-induced emission nanoparticles antibacterial antibacterial differentiation bioactive glass bioprobe guided tissue regeneration (GTR) |
| title | Antibacterial and Fluorescence Staining Properties of an Innovative GTR Membrane Containing 45S5BGs and AIE Molecules In Vitro |
| title_full | Antibacterial and Fluorescence Staining Properties of an Innovative GTR Membrane Containing 45S5BGs and AIE Molecules In Vitro |
| title_fullStr | Antibacterial and Fluorescence Staining Properties of an Innovative GTR Membrane Containing 45S5BGs and AIE Molecules In Vitro |
| title_full_unstemmed | Antibacterial and Fluorescence Staining Properties of an Innovative GTR Membrane Containing 45S5BGs and AIE Molecules In Vitro |
| title_short | Antibacterial and Fluorescence Staining Properties of an Innovative GTR Membrane Containing 45S5BGs and AIE Molecules In Vitro |
| title_sort | antibacterial and fluorescence staining properties of an innovative gtr membrane containing 45s5bgs and aie molecules in vitro |
| topic | aggregation-induced emission nanoparticles antibacterial antibacterial differentiation bioactive glass bioprobe guided tissue regeneration (GTR) |
| url | https://www.mdpi.com/2079-4991/12/4/641 |
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