qpure: A tool to estimate tumor cellularity from genome-wide single-nucleotide polymorphism profiles.
Tumour cellularity, the relative proportion of tumour and normal cells in a sample, affects the sensitivity of mutation detection, copy number analysis, cancer gene expression and methylation profiling. Tumour cellularity is traditionally estimated by pathological review of sectioned specimens; howe...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23049875/?tool=EBI |
| _version_ | 1818435425601060864 |
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| author | Sarah Song Katia Nones David Miller Ivon Harliwong Karin S Kassahn Mark Pinese Marina Pajic Anthony J Gill Amber L Johns Matthew Anderson Oliver Holmes Conrad Leonard Darrin Taylor Scott Wood Qinying Xu Felicity Newell Mark J Cowley Jianmin Wu Peter Wilson Lynn Fink Andrew V Biankin Nic Waddell Sean M Grimmond John V Pearson |
| author_facet | Sarah Song Katia Nones David Miller Ivon Harliwong Karin S Kassahn Mark Pinese Marina Pajic Anthony J Gill Amber L Johns Matthew Anderson Oliver Holmes Conrad Leonard Darrin Taylor Scott Wood Qinying Xu Felicity Newell Mark J Cowley Jianmin Wu Peter Wilson Lynn Fink Andrew V Biankin Nic Waddell Sean M Grimmond John V Pearson |
| author_sort | Sarah Song |
| collection | DOAJ |
| description | Tumour cellularity, the relative proportion of tumour and normal cells in a sample, affects the sensitivity of mutation detection, copy number analysis, cancer gene expression and methylation profiling. Tumour cellularity is traditionally estimated by pathological review of sectioned specimens; however this method is both subjective and prone to error due to heterogeneity within lesions and cellularity differences between the sample viewed during pathological review and tissue used for research purposes. In this paper we describe a statistical model to estimate tumour cellularity from SNP array profiles of paired tumour and normal samples using shifts in SNP allele frequency at regions of loss of heterozygosity (LOH) in the tumour. We also provide qpure, a software implementation of the method. Our experiments showed that there is a medium correlation 0.42 ([Formula: see text]-value=0.0001) between tumor cellularity estimated by qpure and pathology review. Interestingly there is a high correlation 0.87 ([Formula: see text]-value [Formula: see text] 2.2e-16) between cellularity estimates by qpure and deep Ion Torrent sequencing of known somatic KRAS mutations; and a weaker correlation 0.32 ([Formula: see text]-value=0.004) between IonTorrent sequencing and pathology review. This suggests that qpure may be a more accurate predictor of tumour cellularity than pathology review. qpure can be downloaded from https://sourceforge.net/projects/qpure/. |
| first_indexed | 2024-12-14T16:52:41Z |
| format | Article |
| id | doaj.art-0a1169d0232c46ce9e20460b4fa36033 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T16:52:41Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0a1169d0232c46ce9e20460b4fa360332022-12-21T22:54:01ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0179e4583510.1371/journal.pone.0045835qpure: A tool to estimate tumor cellularity from genome-wide single-nucleotide polymorphism profiles.Sarah SongKatia NonesDavid MillerIvon HarliwongKarin S KassahnMark PineseMarina PajicAnthony J GillAmber L JohnsMatthew AndersonOliver HolmesConrad LeonardDarrin TaylorScott WoodQinying XuFelicity NewellMark J CowleyJianmin WuPeter WilsonLynn FinkAndrew V BiankinNic WaddellSean M GrimmondJohn V PearsonTumour cellularity, the relative proportion of tumour and normal cells in a sample, affects the sensitivity of mutation detection, copy number analysis, cancer gene expression and methylation profiling. Tumour cellularity is traditionally estimated by pathological review of sectioned specimens; however this method is both subjective and prone to error due to heterogeneity within lesions and cellularity differences between the sample viewed during pathological review and tissue used for research purposes. In this paper we describe a statistical model to estimate tumour cellularity from SNP array profiles of paired tumour and normal samples using shifts in SNP allele frequency at regions of loss of heterozygosity (LOH) in the tumour. We also provide qpure, a software implementation of the method. Our experiments showed that there is a medium correlation 0.42 ([Formula: see text]-value=0.0001) between tumor cellularity estimated by qpure and pathology review. Interestingly there is a high correlation 0.87 ([Formula: see text]-value [Formula: see text] 2.2e-16) between cellularity estimates by qpure and deep Ion Torrent sequencing of known somatic KRAS mutations; and a weaker correlation 0.32 ([Formula: see text]-value=0.004) between IonTorrent sequencing and pathology review. This suggests that qpure may be a more accurate predictor of tumour cellularity than pathology review. qpure can be downloaded from https://sourceforge.net/projects/qpure/.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23049875/?tool=EBI |
| spellingShingle | Sarah Song Katia Nones David Miller Ivon Harliwong Karin S Kassahn Mark Pinese Marina Pajic Anthony J Gill Amber L Johns Matthew Anderson Oliver Holmes Conrad Leonard Darrin Taylor Scott Wood Qinying Xu Felicity Newell Mark J Cowley Jianmin Wu Peter Wilson Lynn Fink Andrew V Biankin Nic Waddell Sean M Grimmond John V Pearson qpure: A tool to estimate tumor cellularity from genome-wide single-nucleotide polymorphism profiles. PLoS ONE |
| title | qpure: A tool to estimate tumor cellularity from genome-wide single-nucleotide polymorphism profiles. |
| title_full | qpure: A tool to estimate tumor cellularity from genome-wide single-nucleotide polymorphism profiles. |
| title_fullStr | qpure: A tool to estimate tumor cellularity from genome-wide single-nucleotide polymorphism profiles. |
| title_full_unstemmed | qpure: A tool to estimate tumor cellularity from genome-wide single-nucleotide polymorphism profiles. |
| title_short | qpure: A tool to estimate tumor cellularity from genome-wide single-nucleotide polymorphism profiles. |
| title_sort | qpure a tool to estimate tumor cellularity from genome wide single nucleotide polymorphism profiles |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23049875/?tool=EBI |
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