Single vs. Combined Therapeutic Approaches in Rats With Chronic Spinal Cord Injury
The regenerative capability of the central nervous system is limited after traumatic spinal cord injury (SCI) due to intrinsic and extrinsic factors that inhibit spinal cord regeneration, resulting in deficient functional recovery. It has been shown that strategies, such as pre-degenerated periphera...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-03-01
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| Series: | Frontiers in Neurology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fneur.2020.00136/full |
| _version_ | 1811285911862771712 |
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| author | Vinnitsa Buzoianu-Anguiano Jared Rivera-Osorio Sandra Orozco-Suárez Angélica Vega-García Elisa García-Vences Stephanie Sánchez-Torres Ismael Jiménez-Estrada Gabriel Guizar-Sahagún Gabriel Guizar-Sahagún Jose Mondragon-Caso Francisca Fernández-Valverde Ignacio Madrazo Israel Grijalva |
| author_facet | Vinnitsa Buzoianu-Anguiano Jared Rivera-Osorio Sandra Orozco-Suárez Angélica Vega-García Elisa García-Vences Stephanie Sánchez-Torres Ismael Jiménez-Estrada Gabriel Guizar-Sahagún Gabriel Guizar-Sahagún Jose Mondragon-Caso Francisca Fernández-Valverde Ignacio Madrazo Israel Grijalva |
| author_sort | Vinnitsa Buzoianu-Anguiano |
| collection | DOAJ |
| description | The regenerative capability of the central nervous system is limited after traumatic spinal cord injury (SCI) due to intrinsic and extrinsic factors that inhibit spinal cord regeneration, resulting in deficient functional recovery. It has been shown that strategies, such as pre-degenerated peripheral nerve (PPN) grafts or the use of bone marrow stromal cells (BMSCs) or exogenous molecules, such as chondroitinase ABC (ChABC) promote axonal growth and remyelination, resulting in an improvement in locomotor function. These treatments have been primarily assessed in acute injury models. The aim of the present study is to evaluate the ability of several single and combined treatments in order to modify the course of chronic complete SCI in rats. A complete cord transection was performed at the T9 level. One month later, animals were divided into five groups: original injury only (control group), and original injury plus spinal cord re-transection to create a gap to accommodate BMSCs, PPN, PPN + BMSCs, and PPN + BMSCs + ChABC. In comparison with control and single-treatment groups (PPN and BMSCs), combined treatment groups (PPN + BMSCs and PPN + BMSCs + ChABC) showed significative axonal regrowth, as revealed by an increase in GAP-43 and MAP-1B expression in axonal fibers, which correlated with an improvement in locomotor function. In conclusion, the combined therapies tested here improve locomotor function by enhancing axonal regeneration in rats with chronic SCI. Further studies are warranted to refine this promising line of research for clinical purposes. |
| first_indexed | 2024-04-13T02:51:54Z |
| format | Article |
| id | doaj.art-0a1415c5c75747c7a5dba36e56c4e585 |
| institution | Directory Open Access Journal |
| issn | 1664-2295 |
| language | English |
| last_indexed | 2024-04-13T02:51:54Z |
| publishDate | 2020-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neurology |
| spelling | doaj.art-0a1415c5c75747c7a5dba36e56c4e5852022-12-22T03:05:49ZengFrontiers Media S.A.Frontiers in Neurology1664-22952020-03-011110.3389/fneur.2020.00136516292Single vs. Combined Therapeutic Approaches in Rats With Chronic Spinal Cord InjuryVinnitsa Buzoianu-Anguiano0Jared Rivera-Osorio1Sandra Orozco-Suárez2Angélica Vega-García3Elisa García-Vences4Stephanie Sánchez-Torres5Ismael Jiménez-Estrada6Gabriel Guizar-Sahagún7Gabriel Guizar-Sahagún8Jose Mondragon-Caso9Francisca Fernández-Valverde10Ignacio Madrazo11Israel Grijalva12Hospital de Especialidades CMN Siglo XXI IMSS, Unidad de Investigación Médica en Enfermedades Neurologicas, Mexico City, MexicoHospital de Especialidades CMN Siglo XXI IMSS, Unidad de Investigación Médica en Enfermedades Neurologicas, Mexico City, MexicoHospital de Especialidades CMN Siglo XXI IMSS, Unidad de Investigación Médica en Enfermedades Neurologicas, Mexico City, MexicoHospital de Especialidades CMN Siglo XXI IMSS, Unidad de Investigación Médica en Enfermedades Neurologicas, Mexico City, MexicoCentro de Investigación en Ciencias de la Salud, Universidad Anahuac México Campus Norte, Mexico City, MexicoHospital de Especialidades CMN Siglo XXI IMSS, Unidad de Investigación Médica en Enfermedades Neurologicas, Mexico City, MexicoDepartamento de Fisiología, Biofísica y Neurociencias, CINVESTAV, IPN, Mexico City, MexicoHospital de Especialidades CMN Siglo XXI IMSS, Unidad de Investigación Médica en Enfermedades Neurologicas, Mexico City, MexicoDepartamento de Cirugía Experimental, Proyecto Camina AC, Mexico City, MexicoCentro de Investigación en Ciencias de la Salud, Universidad Anahuac México Campus Norte, Mexico City, MexicoLaboratorio de Patología Experimental, Instituto Nacional de Neurología y Neurocirugía, Mexico City, MexicoHospital de Especialidades CMN Siglo XXI IMSS, Unidad de Investigación Médica en Enfermedades Neurologicas, Mexico City, MexicoHospital de Especialidades CMN Siglo XXI IMSS, Unidad de Investigación Médica en Enfermedades Neurologicas, Mexico City, MexicoThe regenerative capability of the central nervous system is limited after traumatic spinal cord injury (SCI) due to intrinsic and extrinsic factors that inhibit spinal cord regeneration, resulting in deficient functional recovery. It has been shown that strategies, such as pre-degenerated peripheral nerve (PPN) grafts or the use of bone marrow stromal cells (BMSCs) or exogenous molecules, such as chondroitinase ABC (ChABC) promote axonal growth and remyelination, resulting in an improvement in locomotor function. These treatments have been primarily assessed in acute injury models. The aim of the present study is to evaluate the ability of several single and combined treatments in order to modify the course of chronic complete SCI in rats. A complete cord transection was performed at the T9 level. One month later, animals were divided into five groups: original injury only (control group), and original injury plus spinal cord re-transection to create a gap to accommodate BMSCs, PPN, PPN + BMSCs, and PPN + BMSCs + ChABC. In comparison with control and single-treatment groups (PPN and BMSCs), combined treatment groups (PPN + BMSCs and PPN + BMSCs + ChABC) showed significative axonal regrowth, as revealed by an increase in GAP-43 and MAP-1B expression in axonal fibers, which correlated with an improvement in locomotor function. In conclusion, the combined therapies tested here improve locomotor function by enhancing axonal regeneration in rats with chronic SCI. Further studies are warranted to refine this promising line of research for clinical purposes.https://www.frontiersin.org/article/10.3389/fneur.2020.00136/fullaxonal regenerationbone marrow stromal cell transplantpre-degeneration peripheral nerve transplantBBB modified scorekinematic analysisfunctional recovery |
| spellingShingle | Vinnitsa Buzoianu-Anguiano Jared Rivera-Osorio Sandra Orozco-Suárez Angélica Vega-García Elisa García-Vences Stephanie Sánchez-Torres Ismael Jiménez-Estrada Gabriel Guizar-Sahagún Gabriel Guizar-Sahagún Jose Mondragon-Caso Francisca Fernández-Valverde Ignacio Madrazo Israel Grijalva Single vs. Combined Therapeutic Approaches in Rats With Chronic Spinal Cord Injury Frontiers in Neurology axonal regeneration bone marrow stromal cell transplant pre-degeneration peripheral nerve transplant BBB modified score kinematic analysis functional recovery |
| title | Single vs. Combined Therapeutic Approaches in Rats With Chronic Spinal Cord Injury |
| title_full | Single vs. Combined Therapeutic Approaches in Rats With Chronic Spinal Cord Injury |
| title_fullStr | Single vs. Combined Therapeutic Approaches in Rats With Chronic Spinal Cord Injury |
| title_full_unstemmed | Single vs. Combined Therapeutic Approaches in Rats With Chronic Spinal Cord Injury |
| title_short | Single vs. Combined Therapeutic Approaches in Rats With Chronic Spinal Cord Injury |
| title_sort | single vs combined therapeutic approaches in rats with chronic spinal cord injury |
| topic | axonal regeneration bone marrow stromal cell transplant pre-degeneration peripheral nerve transplant BBB modified score kinematic analysis functional recovery |
| url | https://www.frontiersin.org/article/10.3389/fneur.2020.00136/full |
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