Transport of antibody into the skin is only partially dependent upon the neonatal Fc-receptor.
The dermis is the portal of entry for most vector-transmitted pathogens, making the host's immune response at this site critical in mitigating the magnitude of infection. For malaria, antibody-mediated neutralization of Plasmodium parasites in the dermis was recently demonstrated. However, surp...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0273960 |
| _version_ | 1797829937604853760 |
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| author | Gibran Nasir Photini Sinnis |
| author_facet | Gibran Nasir Photini Sinnis |
| author_sort | Gibran Nasir |
| collection | DOAJ |
| description | The dermis is the portal of entry for most vector-transmitted pathogens, making the host's immune response at this site critical in mitigating the magnitude of infection. For malaria, antibody-mediated neutralization of Plasmodium parasites in the dermis was recently demonstrated. However, surprisingly little is known about the mechanisms that govern antibody transport into the skin. Since the neonatal Fc receptor (FcRn) has been shown to transcytose IgG into various tissues, we sought to understand its contribution to IgG transport into the skin and antibody-mediated inhibition of Plasmodium parasites following mosquito bite inoculation. Using confocal imaging, we show that the transport of an anti-Langerin mAb into the skin occurs but is only partially reduced in mice lacking FcRn. To understand the relevance of FcRn in the context of malaria infection, we use the rodent parasite Plasmodium berghei and show that passively-administered anti-malarial antibody in FcRn deficient mice, does not reduce parasite burden to the same extent as previously observed in wildtype mice. Overall, our data suggest that FcRn plays a role in the transport of IgG into the skin but is not the major driver of IgG transport into this tissue. These findings have implications for the rational design of antibody-based therapeutics for malaria as well as other vector-transmitted pathogens. |
| first_indexed | 2024-04-09T13:28:08Z |
| format | Article |
| id | doaj.art-0a1a09abae9442d8960136a066a3ac8c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-09T13:28:08Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0a1a09abae9442d8960136a066a3ac8c2023-05-10T05:31:40ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01184e027396010.1371/journal.pone.0273960Transport of antibody into the skin is only partially dependent upon the neonatal Fc-receptor.Gibran NasirPhotini SinnisThe dermis is the portal of entry for most vector-transmitted pathogens, making the host's immune response at this site critical in mitigating the magnitude of infection. For malaria, antibody-mediated neutralization of Plasmodium parasites in the dermis was recently demonstrated. However, surprisingly little is known about the mechanisms that govern antibody transport into the skin. Since the neonatal Fc receptor (FcRn) has been shown to transcytose IgG into various tissues, we sought to understand its contribution to IgG transport into the skin and antibody-mediated inhibition of Plasmodium parasites following mosquito bite inoculation. Using confocal imaging, we show that the transport of an anti-Langerin mAb into the skin occurs but is only partially reduced in mice lacking FcRn. To understand the relevance of FcRn in the context of malaria infection, we use the rodent parasite Plasmodium berghei and show that passively-administered anti-malarial antibody in FcRn deficient mice, does not reduce parasite burden to the same extent as previously observed in wildtype mice. Overall, our data suggest that FcRn plays a role in the transport of IgG into the skin but is not the major driver of IgG transport into this tissue. These findings have implications for the rational design of antibody-based therapeutics for malaria as well as other vector-transmitted pathogens.https://doi.org/10.1371/journal.pone.0273960 |
| spellingShingle | Gibran Nasir Photini Sinnis Transport of antibody into the skin is only partially dependent upon the neonatal Fc-receptor. PLoS ONE |
| title | Transport of antibody into the skin is only partially dependent upon the neonatal Fc-receptor. |
| title_full | Transport of antibody into the skin is only partially dependent upon the neonatal Fc-receptor. |
| title_fullStr | Transport of antibody into the skin is only partially dependent upon the neonatal Fc-receptor. |
| title_full_unstemmed | Transport of antibody into the skin is only partially dependent upon the neonatal Fc-receptor. |
| title_short | Transport of antibody into the skin is only partially dependent upon the neonatal Fc-receptor. |
| title_sort | transport of antibody into the skin is only partially dependent upon the neonatal fc receptor |
| url | https://doi.org/10.1371/journal.pone.0273960 |
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