Droplet-vitrification of Aranda Broga Blue orchid: Role of ascorbic acid on the antioxidant system and genetic fidelity assessments via RAPD and SCoT markers
A droplet-vitrification cryopreservation protocol has been successfully developed for Aranda Broga Blue orchid hybrid using protocorm-like bodies (PLBs). However, maximum growth regeneration percentage was recorded at 5% only based on previous report. Thus, to improve growth recovery of cryopreserve...
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| Format: | Article |
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Elsevier
2020-06-01
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| Series: | Biotechnology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X2030148X |
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| author | Soo Ping Khor Lit Chow Yeow Ranjetta Poobathy Rahmad Zakaria Bee Lynn Chew Sreeramanan Subramaniam |
| author_facet | Soo Ping Khor Lit Chow Yeow Ranjetta Poobathy Rahmad Zakaria Bee Lynn Chew Sreeramanan Subramaniam |
| author_sort | Soo Ping Khor |
| collection | DOAJ |
| description | A droplet-vitrification cryopreservation protocol has been successfully developed for Aranda Broga Blue orchid hybrid using protocorm-like bodies (PLBs). However, maximum growth regeneration percentage was recorded at 5% only based on previous report. Thus, to improve growth recovery of cryopreserved PLBs, cryopreservation stages were supplemented with ascorbic acid, tested at 50, 100 and 150 mg/L. However, results demonstrated that exogenous ascorbic acid was not favorable in regeneration of cryopreserved explants (maximum value of 1.67 % with 50 mg/L ascorbic acid supplementation). Total soluble protein and various antioxidant enzyme activities such as catalase (CAT), superoxide dismutase (SOD) and ascorbate peroxidase (APX) were evaluated after each cryopreservation stages in conjunction with the application of exogenous ascorbic acid. Addition of antioxidant must be carefully evaluated and its application may not guarantee successful growth recovery. RAPD and SCoT molecular analysis confirmed the genetic stability of regenerated cryopreserved PLBs as no polymorphism was detected compared to control PLBs culture. |
| first_indexed | 2024-12-19T14:38:08Z |
| format | Article |
| id | doaj.art-0a1ca3be20d943d4b0532955ac8c16cf |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-12-19T14:38:08Z |
| publishDate | 2020-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-0a1ca3be20d943d4b0532955ac8c16cf2022-12-21T20:17:11ZengElsevierBiotechnology Reports2215-017X2020-06-0126e00448Droplet-vitrification of Aranda Broga Blue orchid: Role of ascorbic acid on the antioxidant system and genetic fidelity assessments via RAPD and SCoT markersSoo Ping Khor0Lit Chow Yeow1Ranjetta Poobathy2Rahmad Zakaria3Bee Lynn Chew4Sreeramanan Subramaniam5School of Biological Sciences, Universiti Sains Malaysia (USM), 11800, Gelugor, Penang, MalaysiaSchool of Biological Sciences, Universiti Sains Malaysia (USM), 11800, Gelugor, Penang, MalaysiaSchool of Biological Sciences, Universiti Sains Malaysia (USM), 11800, Gelugor, Penang, Malaysia; School of Biological Sciences, Quest International University, 30250, Ipoh, Perak, MalaysiaSchool of Biological Sciences, Universiti Sains Malaysia (USM), 11800, Gelugor, Penang, MalaysiaSchool of Biological Sciences, Universiti Sains Malaysia (USM), 11800, Gelugor, Penang, MalaysiaSchool of Biological Sciences, Universiti Sains Malaysia (USM), 11800, Gelugor, Penang, Malaysia; Centre for Chemical Biology, Universiti Sains Malaysia, Bayan Lepas, Penang, Malaysia; Corresponding author at: School of Biological Sciences, Universiti Sains Malaysia (USM), 11800, Gelugor, Penang, Malaysia.A droplet-vitrification cryopreservation protocol has been successfully developed for Aranda Broga Blue orchid hybrid using protocorm-like bodies (PLBs). However, maximum growth regeneration percentage was recorded at 5% only based on previous report. Thus, to improve growth recovery of cryopreserved PLBs, cryopreservation stages were supplemented with ascorbic acid, tested at 50, 100 and 150 mg/L. However, results demonstrated that exogenous ascorbic acid was not favorable in regeneration of cryopreserved explants (maximum value of 1.67 % with 50 mg/L ascorbic acid supplementation). Total soluble protein and various antioxidant enzyme activities such as catalase (CAT), superoxide dismutase (SOD) and ascorbate peroxidase (APX) were evaluated after each cryopreservation stages in conjunction with the application of exogenous ascorbic acid. Addition of antioxidant must be carefully evaluated and its application may not guarantee successful growth recovery. RAPD and SCoT molecular analysis confirmed the genetic stability of regenerated cryopreserved PLBs as no polymorphism was detected compared to control PLBs culture.http://www.sciencedirect.com/science/article/pii/S2215017X2030148XAranda Broga Blue orchidCryopreservationAntioxidant enzymesMolecular markersGenetic fidelity |
| spellingShingle | Soo Ping Khor Lit Chow Yeow Ranjetta Poobathy Rahmad Zakaria Bee Lynn Chew Sreeramanan Subramaniam Droplet-vitrification of Aranda Broga Blue orchid: Role of ascorbic acid on the antioxidant system and genetic fidelity assessments via RAPD and SCoT markers Biotechnology Reports Aranda Broga Blue orchid Cryopreservation Antioxidant enzymes Molecular markers Genetic fidelity |
| title | Droplet-vitrification of Aranda Broga Blue orchid: Role of ascorbic acid on the antioxidant system and genetic fidelity assessments via RAPD and SCoT markers |
| title_full | Droplet-vitrification of Aranda Broga Blue orchid: Role of ascorbic acid on the antioxidant system and genetic fidelity assessments via RAPD and SCoT markers |
| title_fullStr | Droplet-vitrification of Aranda Broga Blue orchid: Role of ascorbic acid on the antioxidant system and genetic fidelity assessments via RAPD and SCoT markers |
| title_full_unstemmed | Droplet-vitrification of Aranda Broga Blue orchid: Role of ascorbic acid on the antioxidant system and genetic fidelity assessments via RAPD and SCoT markers |
| title_short | Droplet-vitrification of Aranda Broga Blue orchid: Role of ascorbic acid on the antioxidant system and genetic fidelity assessments via RAPD and SCoT markers |
| title_sort | droplet vitrification of aranda broga blue orchid role of ascorbic acid on the antioxidant system and genetic fidelity assessments via rapd and scot markers |
| topic | Aranda Broga Blue orchid Cryopreservation Antioxidant enzymes Molecular markers Genetic fidelity |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X2030148X |
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