A Comparative Study Between the Effectiveness of 980 nm Photobiomodulation Delivered by Hand-Piece With Gaussian vs. Flat-Top Profiles on Osteoblasts Maturation
Photobiomodulation (PBM) is a clinically accepted tool in regenerative medicine and dentistry to improve tissue healing and repair and to restore the functional disability. The current in vitro study aimed to investigate the photobiomodulatory effects of 980 nm wavelength (the real energy at the tar...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-02-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fendo.2019.00092/full |
| _version_ | 1828186643987693568 |
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| author | Reem Hanna Reem Hanna Dimitrios Agas Stefano Benedicenti Sara Ferrando Fulvio Laus Vincenzo Cuteri Giovanna Lacava Maria Giovanna Sabbieti Andrea Amaroli Andrea Amaroli |
| author_facet | Reem Hanna Reem Hanna Dimitrios Agas Stefano Benedicenti Sara Ferrando Fulvio Laus Vincenzo Cuteri Giovanna Lacava Maria Giovanna Sabbieti Andrea Amaroli Andrea Amaroli |
| author_sort | Reem Hanna |
| collection | DOAJ |
| description | Photobiomodulation (PBM) is a clinically accepted tool in regenerative medicine and dentistry to improve tissue healing and repair and to restore the functional disability. The current in vitro study aimed to investigate the photobiomodulatory effects of 980 nm wavelength (the real energy at the target: ~0.9 W, ~0.9 W/cm2, 60 s, ~55 J/cm2 and a single energy ~55 J in CW) on MC3T3-E1 pre-osteoblast, delivered with flattop profile in comparison to the standard profile. The laser groupings and their associated energies were: Group 1 - once per week (total energy 110 J); Group 2 - three times per week (alternate day) (total energy 330 J); Group 3 - five times per week (total energy 550 J). The metabolic activity and the osteoblasts maturation were analyzed by alkaline phosphatase assay, alizarin red S histological staining, immunoblot and/or double immunolabeling analysis for Bcl2, Bax, Runx-2, Osx, Dlx5, osteocalcin, and collagen Type 1. Our data, for the first time, prove that laser irradiation of 980 nm wavelength with flat-top beam profile delivery system, compared to standard-Gaussian profile, has improved photobiomodulatory efficacy on pre-osteoblastic cells differentiation. Mechanistically, the irradiation enhances the pre-osteoblast differentiation through activation of Wnt signaling and activation of Smads 2/3-βcatenin pathway. |
| first_indexed | 2024-04-12T07:21:10Z |
| format | Article |
| id | doaj.art-0a1de995f2444a75a4f818c035db856c |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-12T07:21:10Z |
| publishDate | 2019-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-0a1de995f2444a75a4f818c035db856c2022-12-22T03:42:18ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922019-02-011010.3389/fendo.2019.00092431620A Comparative Study Between the Effectiveness of 980 nm Photobiomodulation Delivered by Hand-Piece With Gaussian vs. Flat-Top Profiles on Osteoblasts MaturationReem Hanna0Reem Hanna1Dimitrios Agas2Stefano Benedicenti3Sara Ferrando4Fulvio Laus5Vincenzo Cuteri6Giovanna Lacava7Maria Giovanna Sabbieti8Andrea Amaroli9Andrea Amaroli10Department of Surgical and Diagnostic Sciences, Laser Therapy Centre, University of Genoa, Genoa, ItalyDepartment of Oral Surgery, Dental Institute, King's College Hospital NHS Foundation Trust, London, United KingdomSchool of Biosciences and Veterinary Medicine, University of Camerino, Camerino, ItalyDepartment of Surgical and Diagnostic Sciences, Laser Therapy Centre, University of Genoa, Genoa, ItalyLaboratory of New Model Organism (NeMo LAB), Department of Earth, Environmental and Life Sciences, University of Genoa, Genoa, ItalySchool of Biosciences and Veterinary Medicine, University of Camerino, Camerino, ItalySchool of Biosciences and Veterinary Medicine, University of Camerino, Camerino, ItalySchool of Biosciences and Veterinary Medicine, University of Camerino, Camerino, ItalySchool of Biosciences and Veterinary Medicine, University of Camerino, Camerino, ItalyDepartment of Surgical and Diagnostic Sciences, Laser Therapy Centre, University of Genoa, Genoa, ItalyLaboratory of New Model Organism (NeMo LAB), Department of Earth, Environmental and Life Sciences, University of Genoa, Genoa, ItalyPhotobiomodulation (PBM) is a clinically accepted tool in regenerative medicine and dentistry to improve tissue healing and repair and to restore the functional disability. The current in vitro study aimed to investigate the photobiomodulatory effects of 980 nm wavelength (the real energy at the target: ~0.9 W, ~0.9 W/cm2, 60 s, ~55 J/cm2 and a single energy ~55 J in CW) on MC3T3-E1 pre-osteoblast, delivered with flattop profile in comparison to the standard profile. The laser groupings and their associated energies were: Group 1 - once per week (total energy 110 J); Group 2 - three times per week (alternate day) (total energy 330 J); Group 3 - five times per week (total energy 550 J). The metabolic activity and the osteoblasts maturation were analyzed by alkaline phosphatase assay, alizarin red S histological staining, immunoblot and/or double immunolabeling analysis for Bcl2, Bax, Runx-2, Osx, Dlx5, osteocalcin, and collagen Type 1. Our data, for the first time, prove that laser irradiation of 980 nm wavelength with flat-top beam profile delivery system, compared to standard-Gaussian profile, has improved photobiomodulatory efficacy on pre-osteoblastic cells differentiation. Mechanistically, the irradiation enhances the pre-osteoblast differentiation through activation of Wnt signaling and activation of Smads 2/3-βcatenin pathway.https://www.frontiersin.org/article/10.3389/fendo.2019.00092/fullphotobiomodulationbone regenerationosteoblastLow Level Laser Therapy (LLLT)light therapycell proliferation |
| spellingShingle | Reem Hanna Reem Hanna Dimitrios Agas Stefano Benedicenti Sara Ferrando Fulvio Laus Vincenzo Cuteri Giovanna Lacava Maria Giovanna Sabbieti Andrea Amaroli Andrea Amaroli A Comparative Study Between the Effectiveness of 980 nm Photobiomodulation Delivered by Hand-Piece With Gaussian vs. Flat-Top Profiles on Osteoblasts Maturation Frontiers in Endocrinology photobiomodulation bone regeneration osteoblast Low Level Laser Therapy (LLLT) light therapy cell proliferation |
| title | A Comparative Study Between the Effectiveness of 980 nm Photobiomodulation Delivered by Hand-Piece With Gaussian vs. Flat-Top Profiles on Osteoblasts Maturation |
| title_full | A Comparative Study Between the Effectiveness of 980 nm Photobiomodulation Delivered by Hand-Piece With Gaussian vs. Flat-Top Profiles on Osteoblasts Maturation |
| title_fullStr | A Comparative Study Between the Effectiveness of 980 nm Photobiomodulation Delivered by Hand-Piece With Gaussian vs. Flat-Top Profiles on Osteoblasts Maturation |
| title_full_unstemmed | A Comparative Study Between the Effectiveness of 980 nm Photobiomodulation Delivered by Hand-Piece With Gaussian vs. Flat-Top Profiles on Osteoblasts Maturation |
| title_short | A Comparative Study Between the Effectiveness of 980 nm Photobiomodulation Delivered by Hand-Piece With Gaussian vs. Flat-Top Profiles on Osteoblasts Maturation |
| title_sort | comparative study between the effectiveness of 980 nm photobiomodulation delivered by hand piece with gaussian vs flat top profiles on osteoblasts maturation |
| topic | photobiomodulation bone regeneration osteoblast Low Level Laser Therapy (LLLT) light therapy cell proliferation |
| url | https://www.frontiersin.org/article/10.3389/fendo.2019.00092/full |
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