Preclinical Studies on the Safety and Toxicity of Photoditazine in the Antibacterial Photodynamic Therapy of Uropathogenic Bacteria
The ‘dusting’ technique of lithotripsy for the removal of infected urinary calculi and the wide use of drainage after endoscopic surgery may stimulate spreading of multidrug-resistant bacterial strains. Antibacterial photodynamic therapy (PDT) is one promising method for the elimination these strain...
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MDPI AG
2023-08-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/8/2283 |
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| author | Olga Streltsova Artem Antonyan Nadezhda Ignatova Katerina Yunusova Vadim Elagin Vladislav Kamensky |
| author_facet | Olga Streltsova Artem Antonyan Nadezhda Ignatova Katerina Yunusova Vadim Elagin Vladislav Kamensky |
| author_sort | Olga Streltsova |
| collection | DOAJ |
| description | The ‘dusting’ technique of lithotripsy for the removal of infected urinary calculi and the wide use of drainage after endoscopic surgery may stimulate spreading of multidrug-resistant bacterial strains. Antibacterial photodynamic therapy (PDT) is one promising method for the elimination these strains. The purpose of our study was to evaluate alterations of renal pelvis morphology and renal function in laboratory animals after bactericidal regimens of PDT. Renal pelvises of pigs were filled with Photoditazine and then assessed either by examining the accumulation of Photoditazine in the urothelium or by illumination with a laser at a wavelength of 662 nm. A renal test and a complete blood count was performed to assess a negative effect of the treatment on health. Structural alterations of the kidney tissues were analyzed by histological examination. No photosensitizer fluorescence was detected in the urothelium of the pelvis. Histological study showed that PDT caused minor changes to the urothelium of the renal pelvis but did not affect the underlying connective tissue. No renal function abnormalities were found after PDT. Thus, the study indicates that antibacterial PDT is a safety technique that can complement common antibiotic therapy in the surgical treatment of urolithiasis. |
| first_indexed | 2024-03-09T10:54:56Z |
| format | Article |
| id | doaj.art-0a1ee6bc547a4db6950450bf4d870096 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T10:54:56Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-0a1ee6bc547a4db6950450bf4d8700962023-12-01T01:41:58ZengMDPI AGBiomedicines2227-90592023-08-01118228310.3390/biomedicines11082283Preclinical Studies on the Safety and Toxicity of Photoditazine in the Antibacterial Photodynamic Therapy of Uropathogenic BacteriaOlga Streltsova0Artem Antonyan1Nadezhda Ignatova2Katerina Yunusova3Vadim Elagin4Vladislav Kamensky5Department of Urology Named after E. V. Shakhov, Privolzhsky Research Medical University, 603005 Nizhny Novgorod, RussiaDepartment of Urology Named after E. V. Shakhov, Privolzhsky Research Medical University, 603005 Nizhny Novgorod, RussiaDepartment of Epidemiology, Microbiology and Evidence-Based Medicine, Privolzhsky Research Medical University, 603004 Nizhny Novgorod, RussiaDepartment of Pathological Anatomy, Privolzhsky Research Medical University, 603005 Nizhny Novgorod, RussiaInstitute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 603005 Nizhny Novgorod, RussiaInstitute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 603005 Nizhny Novgorod, RussiaThe ‘dusting’ technique of lithotripsy for the removal of infected urinary calculi and the wide use of drainage after endoscopic surgery may stimulate spreading of multidrug-resistant bacterial strains. Antibacterial photodynamic therapy (PDT) is one promising method for the elimination these strains. The purpose of our study was to evaluate alterations of renal pelvis morphology and renal function in laboratory animals after bactericidal regimens of PDT. Renal pelvises of pigs were filled with Photoditazine and then assessed either by examining the accumulation of Photoditazine in the urothelium or by illumination with a laser at a wavelength of 662 nm. A renal test and a complete blood count was performed to assess a negative effect of the treatment on health. Structural alterations of the kidney tissues were analyzed by histological examination. No photosensitizer fluorescence was detected in the urothelium of the pelvis. Histological study showed that PDT caused minor changes to the urothelium of the renal pelvis but did not affect the underlying connective tissue. No renal function abnormalities were found after PDT. Thus, the study indicates that antibacterial PDT is a safety technique that can complement common antibiotic therapy in the surgical treatment of urolithiasis.https://www.mdpi.com/2227-9059/11/8/2283antibacterial photodynamic therapyPhotoditazinekidneyC-reactive proteincreatinineurea |
| spellingShingle | Olga Streltsova Artem Antonyan Nadezhda Ignatova Katerina Yunusova Vadim Elagin Vladislav Kamensky Preclinical Studies on the Safety and Toxicity of Photoditazine in the Antibacterial Photodynamic Therapy of Uropathogenic Bacteria Biomedicines antibacterial photodynamic therapy Photoditazine kidney C-reactive protein creatinine urea |
| title | Preclinical Studies on the Safety and Toxicity of Photoditazine in the Antibacterial Photodynamic Therapy of Uropathogenic Bacteria |
| title_full | Preclinical Studies on the Safety and Toxicity of Photoditazine in the Antibacterial Photodynamic Therapy of Uropathogenic Bacteria |
| title_fullStr | Preclinical Studies on the Safety and Toxicity of Photoditazine in the Antibacterial Photodynamic Therapy of Uropathogenic Bacteria |
| title_full_unstemmed | Preclinical Studies on the Safety and Toxicity of Photoditazine in the Antibacterial Photodynamic Therapy of Uropathogenic Bacteria |
| title_short | Preclinical Studies on the Safety and Toxicity of Photoditazine in the Antibacterial Photodynamic Therapy of Uropathogenic Bacteria |
| title_sort | preclinical studies on the safety and toxicity of photoditazine in the antibacterial photodynamic therapy of uropathogenic bacteria |
| topic | antibacterial photodynamic therapy Photoditazine kidney C-reactive protein creatinine urea |
| url | https://www.mdpi.com/2227-9059/11/8/2283 |
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