C3HC4-type RING finger protein NbZFP1 is involved in growth and fruit development in Nicotiana benthamiana.
C3HC4-type RING finger proteins constitute a large family in the plant kingdom and play important roles in various physiological processes of plant life. In this study, a C3HC4-type zinc finger gene was isolated from Nicotiana benthamiana. Sequence analysis indicated that the gene encodes a 24-kDa p...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4047095?pdf=render |
| _version_ | 1829106953265086464 |
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| author | Wenxian Wu Zhiwei Cheng Mengjie Liu Xiufen Yang Dewen Qiu |
| author_facet | Wenxian Wu Zhiwei Cheng Mengjie Liu Xiufen Yang Dewen Qiu |
| author_sort | Wenxian Wu |
| collection | DOAJ |
| description | C3HC4-type RING finger proteins constitute a large family in the plant kingdom and play important roles in various physiological processes of plant life. In this study, a C3HC4-type zinc finger gene was isolated from Nicotiana benthamiana. Sequence analysis indicated that the gene encodes a 24-kDa protein with 191 amino acids containing one typical C3HC4-type zinc finger domain; this gene was named NbZFP1. Transient expression of pGDG-NbZFP1 demonstrated that NbZFP1 was localized to the chloroplast, especially in the chloroplasts of cells surrounding leaf stomata. Virus-induced gene silencing (VIGS) analysis indicated that silencing of NbZFP1 hampered fruit development, although the height of the plants was normal. An overexpression construct was then designed and transferred into Nicotiana benthamiana, and PCR and Southern blot showed that the NbZFP1 gene was successfully integrated into the Nicotiana benthamiana genome. The transgenic lines showed typical compactness, with a short internode length and sturdy stems. This is the first report describing the function of a C3HC4-type RING finger protein in tobacco. |
| first_indexed | 2024-12-12T06:31:32Z |
| format | Article |
| id | doaj.art-0a23563b4f734989bcab56cf209b7b39 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T06:31:32Z |
| publishDate | 2014-01-01 |
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| spelling | doaj.art-0a23563b4f734989bcab56cf209b7b392022-12-22T00:34:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0196e9935210.1371/journal.pone.0099352C3HC4-type RING finger protein NbZFP1 is involved in growth and fruit development in Nicotiana benthamiana.Wenxian WuZhiwei ChengMengjie LiuXiufen YangDewen QiuC3HC4-type RING finger proteins constitute a large family in the plant kingdom and play important roles in various physiological processes of plant life. In this study, a C3HC4-type zinc finger gene was isolated from Nicotiana benthamiana. Sequence analysis indicated that the gene encodes a 24-kDa protein with 191 amino acids containing one typical C3HC4-type zinc finger domain; this gene was named NbZFP1. Transient expression of pGDG-NbZFP1 demonstrated that NbZFP1 was localized to the chloroplast, especially in the chloroplasts of cells surrounding leaf stomata. Virus-induced gene silencing (VIGS) analysis indicated that silencing of NbZFP1 hampered fruit development, although the height of the plants was normal. An overexpression construct was then designed and transferred into Nicotiana benthamiana, and PCR and Southern blot showed that the NbZFP1 gene was successfully integrated into the Nicotiana benthamiana genome. The transgenic lines showed typical compactness, with a short internode length and sturdy stems. This is the first report describing the function of a C3HC4-type RING finger protein in tobacco.http://europepmc.org/articles/PMC4047095?pdf=render |
| spellingShingle | Wenxian Wu Zhiwei Cheng Mengjie Liu Xiufen Yang Dewen Qiu C3HC4-type RING finger protein NbZFP1 is involved in growth and fruit development in Nicotiana benthamiana. PLoS ONE |
| title | C3HC4-type RING finger protein NbZFP1 is involved in growth and fruit development in Nicotiana benthamiana. |
| title_full | C3HC4-type RING finger protein NbZFP1 is involved in growth and fruit development in Nicotiana benthamiana. |
| title_fullStr | C3HC4-type RING finger protein NbZFP1 is involved in growth and fruit development in Nicotiana benthamiana. |
| title_full_unstemmed | C3HC4-type RING finger protein NbZFP1 is involved in growth and fruit development in Nicotiana benthamiana. |
| title_short | C3HC4-type RING finger protein NbZFP1 is involved in growth and fruit development in Nicotiana benthamiana. |
| title_sort | c3hc4 type ring finger protein nbzfp1 is involved in growth and fruit development in nicotiana benthamiana |
| url | http://europepmc.org/articles/PMC4047095?pdf=render |
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