Potential use of gold-silver core-shell nanoparticles derived from Garcinia mangostana peel for anticancer compound, protocatechuic acid delivery
Colorectal cancer is one of the most killing cancers and this has become a global problem. Current treatment and anticancer drugs cannot specifically target the cancerous cells, thus causing toxicity towards surrounding non-cancer cells. Hence, there is an urgent need to discover a more target-speci...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2022.997471/full |
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| author | Kar Xin Lee Kamyar Shameli Yuki Nagao Yen Pin Yew Sin-Yeang Teow Hassan Moeini |
| author_facet | Kar Xin Lee Kamyar Shameli Yuki Nagao Yen Pin Yew Sin-Yeang Teow Hassan Moeini |
| author_sort | Kar Xin Lee |
| collection | DOAJ |
| description | Colorectal cancer is one of the most killing cancers and this has become a global problem. Current treatment and anticancer drugs cannot specifically target the cancerous cells, thus causing toxicity towards surrounding non-cancer cells. Hence, there is an urgent need to discover a more target-specific therapeutic agent to overcome this problem. Core-shell nanoparticles have emerged as good candidate for anticancer treatment. This study aimed to synthesize core-shell nanoparticles via green method which utilised crude peels extract of Garcinia mangostana as reducing and stabilising agents for drug delivery. Gold-silver core-shell nanoparticles (Au-AgNPs) were synthesized through seed germination process in which gold nanoparticles acted as the seed. A complete coating was observed through transmission electron microscopy (TEM) when the ratio of AuNPs and AgNPs was 1:9. The size of Au-AgNPs was 38.22 ± 8.41 nm and was mostly spherical in shape. Plant-based drug, protocatechuic acid (PCA) was loaded on the Au-AgNPs to investigate their anticancer activity. In HCT116 colon cancer cells, PCA-loaded Au-AgNPs (IC50 = 10.78 μg/ml) showed higher inhibitory action than the free PCA (IC50= 148.09 μg/ml) and Au-AgNPs alone (IC50= 24.36 μg/ml). Up to 80% inhibition of HCT116 cells was observed after the treatment of PCA-loaded Au-AgNPs at 15.63 μg/ml. The PCA-loaded Au-AgNPs also showed a better selectivity towards HCT116 compared to CCD112 colon normal cells when tested at the same concentrations. These findings suggest that Au-AgNPs system can be used as a potent nanocarrier to combat cancerous cells by offering additional anticancer properties to the loaded drug. |
| first_indexed | 2024-04-12T13:32:34Z |
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| id | doaj.art-0a23cf48d43a4c64b628e6d964fbd265 |
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| issn | 2296-889X |
| language | English |
| last_indexed | 2024-04-12T13:32:34Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-0a23cf48d43a4c64b628e6d964fbd2652022-12-22T03:31:07ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2022-10-01910.3389/fmolb.2022.997471997471Potential use of gold-silver core-shell nanoparticles derived from Garcinia mangostana peel for anticancer compound, protocatechuic acid deliveryKar Xin Lee0Kamyar Shameli1Yuki Nagao2Yen Pin Yew3Sin-Yeang Teow4Hassan Moeini5Malaysia-Japan International Institute of Technology (MJIIT), Universiti Teknologi Malaysia, Kuala Lumpur, MalaysiaMalaysia-Japan International Institute of Technology (MJIIT), Universiti Teknologi Malaysia, Kuala Lumpur, MalaysiaSchool of Materials Science, Japan Advanced Institute of Science and Technology (JAIST), Nomi, JapanMalaysia-Japan International Institute of Technology (MJIIT), Universiti Teknologi Malaysia, Kuala Lumpur, MalaysiaDepartment of Medical Sciences, School of Medical and Life Sciences (SMLS), Sunway University, Jalan Universiti, Bandar Sunway, Selangor Darul Ehsan, MalaysiaSchool of Medicine, Institute of Virology, Technical University of Munich, Munich, GermanyColorectal cancer is one of the most killing cancers and this has become a global problem. Current treatment and anticancer drugs cannot specifically target the cancerous cells, thus causing toxicity towards surrounding non-cancer cells. Hence, there is an urgent need to discover a more target-specific therapeutic agent to overcome this problem. Core-shell nanoparticles have emerged as good candidate for anticancer treatment. This study aimed to synthesize core-shell nanoparticles via green method which utilised crude peels extract of Garcinia mangostana as reducing and stabilising agents for drug delivery. Gold-silver core-shell nanoparticles (Au-AgNPs) were synthesized through seed germination process in which gold nanoparticles acted as the seed. A complete coating was observed through transmission electron microscopy (TEM) when the ratio of AuNPs and AgNPs was 1:9. The size of Au-AgNPs was 38.22 ± 8.41 nm and was mostly spherical in shape. Plant-based drug, protocatechuic acid (PCA) was loaded on the Au-AgNPs to investigate their anticancer activity. In HCT116 colon cancer cells, PCA-loaded Au-AgNPs (IC50 = 10.78 μg/ml) showed higher inhibitory action than the free PCA (IC50= 148.09 μg/ml) and Au-AgNPs alone (IC50= 24.36 μg/ml). Up to 80% inhibition of HCT116 cells was observed after the treatment of PCA-loaded Au-AgNPs at 15.63 μg/ml. The PCA-loaded Au-AgNPs also showed a better selectivity towards HCT116 compared to CCD112 colon normal cells when tested at the same concentrations. These findings suggest that Au-AgNPs system can be used as a potent nanocarrier to combat cancerous cells by offering additional anticancer properties to the loaded drug.https://www.frontiersin.org/articles/10.3389/fmolb.2022.997471/fullcolorectal cancergreen synthesiscore-shell nanoparticlesgold-silverprotocatechuic aciddrug delivery |
| spellingShingle | Kar Xin Lee Kamyar Shameli Yuki Nagao Yen Pin Yew Sin-Yeang Teow Hassan Moeini Potential use of gold-silver core-shell nanoparticles derived from Garcinia mangostana peel for anticancer compound, protocatechuic acid delivery Frontiers in Molecular Biosciences colorectal cancer green synthesis core-shell nanoparticles gold-silver protocatechuic acid drug delivery |
| title | Potential use of gold-silver core-shell nanoparticles derived from Garcinia mangostana peel for anticancer compound, protocatechuic acid delivery |
| title_full | Potential use of gold-silver core-shell nanoparticles derived from Garcinia mangostana peel for anticancer compound, protocatechuic acid delivery |
| title_fullStr | Potential use of gold-silver core-shell nanoparticles derived from Garcinia mangostana peel for anticancer compound, protocatechuic acid delivery |
| title_full_unstemmed | Potential use of gold-silver core-shell nanoparticles derived from Garcinia mangostana peel for anticancer compound, protocatechuic acid delivery |
| title_short | Potential use of gold-silver core-shell nanoparticles derived from Garcinia mangostana peel for anticancer compound, protocatechuic acid delivery |
| title_sort | potential use of gold silver core shell nanoparticles derived from garcinia mangostana peel for anticancer compound protocatechuic acid delivery |
| topic | colorectal cancer green synthesis core-shell nanoparticles gold-silver protocatechuic acid drug delivery |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2022.997471/full |
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