The Transcriptional Response of Hybrid Poplar (Populus trichocarpa x P. deltoids) to Infection by Melampsora medusae Leaf Rust Involves Induction of Flavonoid Pathway Genes Leading to the Accumulation of Proanthocyanidins
The transcriptional response of hybrid poplar (Populus trichocarpa × P. deltoids) to poplar leaf rust (Melampsora medusae) infection was studied using the Populus 15.5K cDNA microarray. Pronounced changes in the transcriptome were observed, with approximately 20% of genes on the array showing either...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2007-07-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-7-0816 |
| _version_ | 1811261278542364672 |
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| author | Manoela Miranda Steven G. Ralph Robin Mellway Rick White Michele C. Heath Jörg Bohlmann C. Peter Constabel |
| author_facet | Manoela Miranda Steven G. Ralph Robin Mellway Rick White Michele C. Heath Jörg Bohlmann C. Peter Constabel |
| author_sort | Manoela Miranda |
| collection | DOAJ |
| description | The transcriptional response of hybrid poplar (Populus trichocarpa × P. deltoids) to poplar leaf rust (Melampsora medusae) infection was studied using the Populus 15.5K cDNA microarray. Pronounced changes in the transcriptome were observed, with approximately 20% of genes on the array showing either induction or repression of transcription within the 9-day infection timecourse. A small number of pathogen-defense genes encoding PR-1, chitinases, and other pathogenesis-related proteins were consistently upregulated throughout the experimental period, but most genes were affected only at individual timepoints. The largest number of changes in gene expression was observed late in the infection at 6 to 9 days postinoculation (dpi). At these timepoints, genes encoding enzymes required for proanthocyanidin (condensed tannin) synthesis were upregulated dramatically. Phytochemical analysis confirmed that, late in the infection, proanthocyanidin levels increased in infected leaves. Strongly M. medusae-repressed genes at 9 dpi included previously characterized wound- and herbivore-induced defense genes, which suggests antagonism between the tree responses to insect feeding and M. medusae infection. In this highly compatible plant-pathogen interaction, we postulate that the biotrophic pathogen evades detection and suppresses early host responses. |
| first_indexed | 2024-04-12T19:01:39Z |
| format | Article |
| id | doaj.art-0a23d1816d4e40efb90b3197f0f8e642 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-12T19:01:39Z |
| publishDate | 2007-07-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-0a23d1816d4e40efb90b3197f0f8e6422022-12-22T03:20:08ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062007-07-0120781683110.1094/MPMI-20-7-0816The Transcriptional Response of Hybrid Poplar (Populus trichocarpa x P. deltoids) to Infection by Melampsora medusae Leaf Rust Involves Induction of Flavonoid Pathway Genes Leading to the Accumulation of ProanthocyanidinsManoela MirandaSteven G. RalphRobin MellwayRick WhiteMichele C. HeathJörg BohlmannC. Peter ConstabelThe transcriptional response of hybrid poplar (Populus trichocarpa × P. deltoids) to poplar leaf rust (Melampsora medusae) infection was studied using the Populus 15.5K cDNA microarray. Pronounced changes in the transcriptome were observed, with approximately 20% of genes on the array showing either induction or repression of transcription within the 9-day infection timecourse. A small number of pathogen-defense genes encoding PR-1, chitinases, and other pathogenesis-related proteins were consistently upregulated throughout the experimental period, but most genes were affected only at individual timepoints. The largest number of changes in gene expression was observed late in the infection at 6 to 9 days postinoculation (dpi). At these timepoints, genes encoding enzymes required for proanthocyanidin (condensed tannin) synthesis were upregulated dramatically. Phytochemical analysis confirmed that, late in the infection, proanthocyanidin levels increased in infected leaves. Strongly M. medusae-repressed genes at 9 dpi included previously characterized wound- and herbivore-induced defense genes, which suggests antagonism between the tree responses to insect feeding and M. medusae infection. In this highly compatible plant-pathogen interaction, we postulate that the biotrophic pathogen evades detection and suppresses early host responses.https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-7-0816condensed tanninsforest tent caterpillarfungal pathogenphenylpropanoid metabolism |
| spellingShingle | Manoela Miranda Steven G. Ralph Robin Mellway Rick White Michele C. Heath Jörg Bohlmann C. Peter Constabel The Transcriptional Response of Hybrid Poplar (Populus trichocarpa x P. deltoids) to Infection by Melampsora medusae Leaf Rust Involves Induction of Flavonoid Pathway Genes Leading to the Accumulation of Proanthocyanidins Molecular Plant-Microbe Interactions condensed tannins forest tent caterpillar fungal pathogen phenylpropanoid metabolism |
| title | The Transcriptional Response of Hybrid Poplar (Populus trichocarpa x P. deltoids) to Infection by Melampsora medusae Leaf Rust Involves Induction of Flavonoid Pathway Genes Leading to the Accumulation of Proanthocyanidins |
| title_full | The Transcriptional Response of Hybrid Poplar (Populus trichocarpa x P. deltoids) to Infection by Melampsora medusae Leaf Rust Involves Induction of Flavonoid Pathway Genes Leading to the Accumulation of Proanthocyanidins |
| title_fullStr | The Transcriptional Response of Hybrid Poplar (Populus trichocarpa x P. deltoids) to Infection by Melampsora medusae Leaf Rust Involves Induction of Flavonoid Pathway Genes Leading to the Accumulation of Proanthocyanidins |
| title_full_unstemmed | The Transcriptional Response of Hybrid Poplar (Populus trichocarpa x P. deltoids) to Infection by Melampsora medusae Leaf Rust Involves Induction of Flavonoid Pathway Genes Leading to the Accumulation of Proanthocyanidins |
| title_short | The Transcriptional Response of Hybrid Poplar (Populus trichocarpa x P. deltoids) to Infection by Melampsora medusae Leaf Rust Involves Induction of Flavonoid Pathway Genes Leading to the Accumulation of Proanthocyanidins |
| title_sort | transcriptional response of hybrid poplar populus trichocarpa x p deltoids to infection by melampsora medusae leaf rust involves induction of flavonoid pathway genes leading to the accumulation of proanthocyanidins |
| topic | condensed tannins forest tent caterpillar fungal pathogen phenylpropanoid metabolism |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-7-0816 |
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