Optimizing Platelet-Rich Plasma: Spin Time and Sample Source
The buff-colored layer separating the plasma from red blood cells (RBCs) in centrifuged blood was named the “buffy coat” in the late 19th century. The division of platelets (PLTs) and leukocytes (WBCs) between the buffy coat, plasma, and RBC layers in centrifuged blood has not been described before....
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/10/11/1270 |
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| author | Theodore E. Harrison Jannice Bowler Chin-I Cheng Kenneth Dean Reeves |
| author_facet | Theodore E. Harrison Jannice Bowler Chin-I Cheng Kenneth Dean Reeves |
| author_sort | Theodore E. Harrison |
| collection | DOAJ |
| description | The buff-colored layer separating the plasma from red blood cells (RBCs) in centrifuged blood was named the “buffy coat” in the late 19th century. The division of platelets (PLTs) and leukocytes (WBCs) between the buffy coat, plasma, and RBC layers in centrifuged blood has not been described before. In this study, we centrifuged 8.5 mL anticoagulated blood samples at 1000× <i>g</i> for 1, 2, 3, 5, 10, and 20 min. We then divided each sample into ten layers and analyzed each layer for cellular composition and mean platelet volume (MPV). Our results show that even after 20 min of centrifugation, about 15% of platelets remain in the plasma layers and 65% in the RBC layers. We found that the platelet count achieved from aspiration of 1 mL volume was optimal, with aspiration beginning 1/2 mL below the buffy coat and extending 1/2 mL above the buffy coat rather than beginning at the buffy coat itself and aspirating only plasma. Using this method of aspiration, we found that the total platelet count means reached a maximum in the 1 mL around the buffy coat after only 5 min of centrifugation. |
| first_indexed | 2024-03-09T17:01:12Z |
| format | Article |
| id | doaj.art-0a26f40f26bf4c41a1102eba2c269d46 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-09T17:01:12Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-0a26f40f26bf4c41a1102eba2c269d462023-11-24T14:29:45ZengMDPI AGBioengineering2306-53542023-10-011011127010.3390/bioengineering10111270Optimizing Platelet-Rich Plasma: Spin Time and Sample SourceTheodore E. Harrison0Jannice Bowler1Chin-I Cheng2Kenneth Dean Reeves3Independent Researcher, Victoria, BC V8L 5K1, CanadaIndependent Researcher, Victoria, BC V9E 1J5, CanadaDepartment of Statistics, Actuarial and Data Science, Central Michigan University, Mt. Pleasant, MI 48859, USAIndependent Researcher, Roeland Park, KS 66205, USAThe buff-colored layer separating the plasma from red blood cells (RBCs) in centrifuged blood was named the “buffy coat” in the late 19th century. The division of platelets (PLTs) and leukocytes (WBCs) between the buffy coat, plasma, and RBC layers in centrifuged blood has not been described before. In this study, we centrifuged 8.5 mL anticoagulated blood samples at 1000× <i>g</i> for 1, 2, 3, 5, 10, and 20 min. We then divided each sample into ten layers and analyzed each layer for cellular composition and mean platelet volume (MPV). Our results show that even after 20 min of centrifugation, about 15% of platelets remain in the plasma layers and 65% in the RBC layers. We found that the platelet count achieved from aspiration of 1 mL volume was optimal, with aspiration beginning 1/2 mL below the buffy coat and extending 1/2 mL above the buffy coat rather than beginning at the buffy coat itself and aspirating only plasma. Using this method of aspiration, we found that the total platelet count means reached a maximum in the 1 mL around the buffy coat after only 5 min of centrifugation.https://www.mdpi.com/2306-5354/10/11/1270plateletsplatelet-rich plasmabuffy coat |
| spellingShingle | Theodore E. Harrison Jannice Bowler Chin-I Cheng Kenneth Dean Reeves Optimizing Platelet-Rich Plasma: Spin Time and Sample Source Bioengineering platelets platelet-rich plasma buffy coat |
| title | Optimizing Platelet-Rich Plasma: Spin Time and Sample Source |
| title_full | Optimizing Platelet-Rich Plasma: Spin Time and Sample Source |
| title_fullStr | Optimizing Platelet-Rich Plasma: Spin Time and Sample Source |
| title_full_unstemmed | Optimizing Platelet-Rich Plasma: Spin Time and Sample Source |
| title_short | Optimizing Platelet-Rich Plasma: Spin Time and Sample Source |
| title_sort | optimizing platelet rich plasma spin time and sample source |
| topic | platelets platelet-rich plasma buffy coat |
| url | https://www.mdpi.com/2306-5354/10/11/1270 |
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