AAU-Specific RNA Cleavage Mediated by MazF Toxin Endoribonuclease Conserved in Nitrosomonas europaea
Nitrosomonas europaea carries numerous toxin-antitoxin systems. However, despite the abundant representation in its chromosome, studies have not surveyed the underlying molecular functions in detail, and their biological roles remain enigmatic. In the present study, we found that a chromosomally-enc...
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| Format: | Article |
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MDPI AG
2016-06-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/8/6/174 |
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| author | Tatsuki Miyamoto Akiko Yokota Satoshi Tsuneda Naohiro Noda |
| author_facet | Tatsuki Miyamoto Akiko Yokota Satoshi Tsuneda Naohiro Noda |
| author_sort | Tatsuki Miyamoto |
| collection | DOAJ |
| description | Nitrosomonas europaea carries numerous toxin-antitoxin systems. However, despite the abundant representation in its chromosome, studies have not surveyed the underlying molecular functions in detail, and their biological roles remain enigmatic. In the present study, we found that a chromosomally-encoded MazF family member, predicted at the locus NE1181, is a functional toxin endoribonuclease, and constitutes a toxin-antitoxin system, together with its cognate antitoxin, MazE. Massive parallel sequencing provided strong evidence that this toxin endoribonuclease exhibits RNA cleavage activity, primarily against the AAU triplet. This sequence-specificity was supported by the results of fluorometric assays. Our results indicate that N. europaea alters the translation profile and regulates its growth using the MazF family of endoribonuclease under certain stressful conditions. |
| first_indexed | 2024-04-11T12:08:02Z |
| format | Article |
| id | doaj.art-0a27ef89f55f476296826f2ac73ec0ea |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T12:08:02Z |
| publishDate | 2016-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-0a27ef89f55f476296826f2ac73ec0ea2022-12-22T04:24:40ZengMDPI AGToxins2072-66512016-06-018617410.3390/toxins8060174toxins8060174AAU-Specific RNA Cleavage Mediated by MazF Toxin Endoribonuclease Conserved in Nitrosomonas europaeaTatsuki Miyamoto0Akiko Yokota1Satoshi Tsuneda2Naohiro Noda3Department of Life Science and Medical Bioscience, Waseda University, Shinjuku-ku, Tokyo 162-8480, JapanBiomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, JapanDepartment of Life Science and Medical Bioscience, Waseda University, Shinjuku-ku, Tokyo 162-8480, JapanDepartment of Life Science and Medical Bioscience, Waseda University, Shinjuku-ku, Tokyo 162-8480, JapanNitrosomonas europaea carries numerous toxin-antitoxin systems. However, despite the abundant representation in its chromosome, studies have not surveyed the underlying molecular functions in detail, and their biological roles remain enigmatic. In the present study, we found that a chromosomally-encoded MazF family member, predicted at the locus NE1181, is a functional toxin endoribonuclease, and constitutes a toxin-antitoxin system, together with its cognate antitoxin, MazE. Massive parallel sequencing provided strong evidence that this toxin endoribonuclease exhibits RNA cleavage activity, primarily against the AAU triplet. This sequence-specificity was supported by the results of fluorometric assays. Our results indicate that N. europaea alters the translation profile and regulates its growth using the MazF family of endoribonuclease under certain stressful conditions.http://www.mdpi.com/2072-6651/8/6/174Nitrosomonas europaeatoxin-antitoxin systemMazFsequence-specific endoribonuclease |
| spellingShingle | Tatsuki Miyamoto Akiko Yokota Satoshi Tsuneda Naohiro Noda AAU-Specific RNA Cleavage Mediated by MazF Toxin Endoribonuclease Conserved in Nitrosomonas europaea Toxins Nitrosomonas europaea toxin-antitoxin system MazF sequence-specific endoribonuclease |
| title | AAU-Specific RNA Cleavage Mediated by MazF Toxin Endoribonuclease Conserved in Nitrosomonas europaea |
| title_full | AAU-Specific RNA Cleavage Mediated by MazF Toxin Endoribonuclease Conserved in Nitrosomonas europaea |
| title_fullStr | AAU-Specific RNA Cleavage Mediated by MazF Toxin Endoribonuclease Conserved in Nitrosomonas europaea |
| title_full_unstemmed | AAU-Specific RNA Cleavage Mediated by MazF Toxin Endoribonuclease Conserved in Nitrosomonas europaea |
| title_short | AAU-Specific RNA Cleavage Mediated by MazF Toxin Endoribonuclease Conserved in Nitrosomonas europaea |
| title_sort | aau specific rna cleavage mediated by mazf toxin endoribonuclease conserved in nitrosomonas europaea |
| topic | Nitrosomonas europaea toxin-antitoxin system MazF sequence-specific endoribonuclease |
| url | http://www.mdpi.com/2072-6651/8/6/174 |
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