Bacterial Antigens Reduced the Inhibition Effect of Capsaicin on Cal 27 Oral Cancer Cell Proliferation
Oral cancer is a major global health problem with high incidence and low survival rates. The oral cavity contains biofilms as dental plaques that harbour both Gram-negative and Gram-positive bacterial antigens, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), respectively. LPS and LTA are known...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/16/8686 |
| _version_ | 1797523544156930048 |
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| author | Rajdeep Chakraborty Karen Vickery Charbel Darido Shoba Ranganathan Honghua Hu |
| author_facet | Rajdeep Chakraborty Karen Vickery Charbel Darido Shoba Ranganathan Honghua Hu |
| author_sort | Rajdeep Chakraborty |
| collection | DOAJ |
| description | Oral cancer is a major global health problem with high incidence and low survival rates. The oral cavity contains biofilms as dental plaques that harbour both Gram-negative and Gram-positive bacterial antigens, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), respectively. LPS and LTA are known to stimulate cancer cell growth, and the bioactive phytochemical capsaicin has been reported to reverse this effect. Here, we tested the efficacy of oral cancer chemotherapy treatment with capsaicin in the presence of LPS, LTA or the combination of both antigens. LPS and LTA were administered to Cal 27 oral cancer cells prior to and/or concurrently with capsaicin, and the treatment efficacy was evaluated by measuring cell proliferation and apoptotic cell death. We found that while capsaicin inhibits oral cancer cell proliferation and metabolism (MT Glo assay) and increases cell death (Trypan blue exclusion assay and Caspase 3/7 expression), its anti-cancer effect was significantly reduced on cells that are either primed or exposed to the bacterial antigens. Capsaicin treatment significantly increased oral cancer cells’ suppressor of cytokine signalling 3 gene expression. This increase was reversed in the presence of bacterial antigens during treatment. Our data establish a rationale for clinical consideration of bacterial antigens that may interfere with the treatment efficacy of oral cancer. |
| first_indexed | 2024-03-10T08:44:29Z |
| format | Article |
| id | doaj.art-0a29eabbdde942ddb8e56a8e802d81c5 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T08:44:29Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0a29eabbdde942ddb8e56a8e802d81c52023-11-22T07:58:57ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-012216868610.3390/ijms22168686Bacterial Antigens Reduced the Inhibition Effect of Capsaicin on Cal 27 Oral Cancer Cell ProliferationRajdeep Chakraborty0Karen Vickery1Charbel Darido2Shoba Ranganathan3Honghua Hu4Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, NSW 2109, AustraliaFaculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, NSW 2109, AustraliaPeter MacCallum Cancer Centre, Melbourne, VIC 3000, AustraliaApplied Biosciences, Faculty of Science and Engineering, Macquarie University, Sydney, NSW 2109, AustraliaFaculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, NSW 2109, AustraliaOral cancer is a major global health problem with high incidence and low survival rates. The oral cavity contains biofilms as dental plaques that harbour both Gram-negative and Gram-positive bacterial antigens, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), respectively. LPS and LTA are known to stimulate cancer cell growth, and the bioactive phytochemical capsaicin has been reported to reverse this effect. Here, we tested the efficacy of oral cancer chemotherapy treatment with capsaicin in the presence of LPS, LTA or the combination of both antigens. LPS and LTA were administered to Cal 27 oral cancer cells prior to and/or concurrently with capsaicin, and the treatment efficacy was evaluated by measuring cell proliferation and apoptotic cell death. We found that while capsaicin inhibits oral cancer cell proliferation and metabolism (MT Glo assay) and increases cell death (Trypan blue exclusion assay and Caspase 3/7 expression), its anti-cancer effect was significantly reduced on cells that are either primed or exposed to the bacterial antigens. Capsaicin treatment significantly increased oral cancer cells’ suppressor of cytokine signalling 3 gene expression. This increase was reversed in the presence of bacterial antigens during treatment. Our data establish a rationale for clinical consideration of bacterial antigens that may interfere with the treatment efficacy of oral cancer.https://www.mdpi.com/1422-0067/22/16/8686lipopolysaccharidelipoteichoic acidoral cancercapsaicinproliferationapoptosis |
| spellingShingle | Rajdeep Chakraborty Karen Vickery Charbel Darido Shoba Ranganathan Honghua Hu Bacterial Antigens Reduced the Inhibition Effect of Capsaicin on Cal 27 Oral Cancer Cell Proliferation International Journal of Molecular Sciences lipopolysaccharide lipoteichoic acid oral cancer capsaicin proliferation apoptosis |
| title | Bacterial Antigens Reduced the Inhibition Effect of Capsaicin on Cal 27 Oral Cancer Cell Proliferation |
| title_full | Bacterial Antigens Reduced the Inhibition Effect of Capsaicin on Cal 27 Oral Cancer Cell Proliferation |
| title_fullStr | Bacterial Antigens Reduced the Inhibition Effect of Capsaicin on Cal 27 Oral Cancer Cell Proliferation |
| title_full_unstemmed | Bacterial Antigens Reduced the Inhibition Effect of Capsaicin on Cal 27 Oral Cancer Cell Proliferation |
| title_short | Bacterial Antigens Reduced the Inhibition Effect of Capsaicin on Cal 27 Oral Cancer Cell Proliferation |
| title_sort | bacterial antigens reduced the inhibition effect of capsaicin on cal 27 oral cancer cell proliferation |
| topic | lipopolysaccharide lipoteichoic acid oral cancer capsaicin proliferation apoptosis |
| url | https://www.mdpi.com/1422-0067/22/16/8686 |
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