Exogenous hormones influence Brassica napus leaf cuticular wax deposition and cuticle function
Background Cuticular waxes cover plant surface and play important roles in protecting plants from abiotic and biotic stresses. The variations of wax deposition and chemical compositions under changing environments have been shown to be related to plant adaptations. However, it is still not clear whe...
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PeerJ Inc.
2020-06-01
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| Online Access: | https://peerj.com/articles/9264.pdf |
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| author | Zheng Yuan Youwei Jiang Yuhua Liu Yi Xu Shuai Li Yanjun Guo Reinhard Jetter Yu Ni |
| author_facet | Zheng Yuan Youwei Jiang Yuhua Liu Yi Xu Shuai Li Yanjun Guo Reinhard Jetter Yu Ni |
| author_sort | Zheng Yuan |
| collection | DOAJ |
| description | Background Cuticular waxes cover plant surface and play important roles in protecting plants from abiotic and biotic stresses. The variations of wax deposition and chemical compositions under changing environments have been shown to be related to plant adaptations. However, it is still not clear whether the wax depositions could be adjusted to increase plant adaptations to stressed conditions. Methods In this study, exogenous methyl jasmonate (MeJA), the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) and salicylic acid (SA) were applied to test their effects on cuticular wax deposition in two Brassica napus cultivars, Zhongshuang 9 (ZS9, low wax coverage ) and Yuyou 19 (YY19, high wax coverage). Next, we measured the water loss rate and the transcriptional expression of genes involved in wax biosynthesis as well as genes related to disease defense. Results Seven wax compound classes, including fatty acids, aldehydes, alkanes, secondary alcohols, ketones, and unbranched as well as branched primary alcohols, were identified in B. napus leaf wax mixtures. MeJA, SA and ACC treatments had no significant effect on total wax amounts in YY19, whereas ACC reduced total wax amounts in ZS9. Overall, hormone treatments led to an increase in the amounts of aldehydes and ketones, and a decrease of secondary alcohol in ZS9, whereas they led to a decrease of alkane amounts and an increase of secondary alcohol amounts in YY19. Concomitantly, both cultivars also exhibited different changes in cuticle permeability, with leaf water loss rate per 15 min increased from 1.57% (averaged across treatments) at 1.57% (averaged across treatments) at 15 min to 3.12% at 30 min for ZS9 (except for ACC treated plant) and decreased for YY19. MeJA-treated plants of both cultivars relatively had higher water loss rate per 15 min when compared to other treatments. Conclusion. Our findings that B. napus leaf wax composition and cuticle permeability are altered by exogenous SA, MeJA and ACC suggest that the hormone treatments affect wax composition, and that the changes in wax profiles would cause changes in cuticle permeability. |
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| language | English |
| last_indexed | 2024-03-09T07:59:16Z |
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| spelling | doaj.art-0a2af54dd67e402eb16965687edbe4bf2023-12-03T00:47:49ZengPeerJ Inc.PeerJ2167-83592020-06-018e926410.7717/peerj.9264Exogenous hormones influence Brassica napus leaf cuticular wax deposition and cuticle functionZheng Yuan0Youwei Jiang1Yuhua Liu2Yi Xu3Shuai Li4Yanjun Guo5Reinhard Jetter6Yu Ni7College of Agronomy and Biotechnology, Academy of Agricultural Sciences, Southwest University, Chongqing, ChinaCollege of Agronomy and Biotechnology, Academy of Agricultural Sciences, Southwest University, Chongqing, ChinaCollege of Agronomy and Biotechnology, Academy of Agricultural Sciences, Southwest University, Chongqing, ChinaCollege of Agronomy and Biotechnology, Academy of Agricultural Sciences, Southwest University, Chongqing, ChinaCollege of Agronomy and Biotechnology, Academy of Agricultural Sciences, Southwest University, Chongqing, ChinaCollege of Agronomy and Biotechnology, Academy of Agricultural Sciences, Southwest University, Chongqing, ChinaDepartment of Botany, University of British Columbia, Vancouver, CanadaCollege of Agronomy and Biotechnology, Academy of Agricultural Sciences, Southwest University, Chongqing, ChinaBackground Cuticular waxes cover plant surface and play important roles in protecting plants from abiotic and biotic stresses. The variations of wax deposition and chemical compositions under changing environments have been shown to be related to plant adaptations. However, it is still not clear whether the wax depositions could be adjusted to increase plant adaptations to stressed conditions. Methods In this study, exogenous methyl jasmonate (MeJA), the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) and salicylic acid (SA) were applied to test their effects on cuticular wax deposition in two Brassica napus cultivars, Zhongshuang 9 (ZS9, low wax coverage ) and Yuyou 19 (YY19, high wax coverage). Next, we measured the water loss rate and the transcriptional expression of genes involved in wax biosynthesis as well as genes related to disease defense. Results Seven wax compound classes, including fatty acids, aldehydes, alkanes, secondary alcohols, ketones, and unbranched as well as branched primary alcohols, were identified in B. napus leaf wax mixtures. MeJA, SA and ACC treatments had no significant effect on total wax amounts in YY19, whereas ACC reduced total wax amounts in ZS9. Overall, hormone treatments led to an increase in the amounts of aldehydes and ketones, and a decrease of secondary alcohol in ZS9, whereas they led to a decrease of alkane amounts and an increase of secondary alcohol amounts in YY19. Concomitantly, both cultivars also exhibited different changes in cuticle permeability, with leaf water loss rate per 15 min increased from 1.57% (averaged across treatments) at 1.57% (averaged across treatments) at 15 min to 3.12% at 30 min for ZS9 (except for ACC treated plant) and decreased for YY19. MeJA-treated plants of both cultivars relatively had higher water loss rate per 15 min when compared to other treatments. Conclusion. Our findings that B. napus leaf wax composition and cuticle permeability are altered by exogenous SA, MeJA and ACC suggest that the hormone treatments affect wax composition, and that the changes in wax profiles would cause changes in cuticle permeability.https://peerj.com/articles/9264.pdfCuticular waxPhytohormoneWater loss ratePermeabilityBrassica napus |
| spellingShingle | Zheng Yuan Youwei Jiang Yuhua Liu Yi Xu Shuai Li Yanjun Guo Reinhard Jetter Yu Ni Exogenous hormones influence Brassica napus leaf cuticular wax deposition and cuticle function PeerJ Cuticular wax Phytohormone Water loss rate Permeability Brassica napus |
| title | Exogenous hormones influence Brassica napus leaf cuticular wax deposition and cuticle function |
| title_full | Exogenous hormones influence Brassica napus leaf cuticular wax deposition and cuticle function |
| title_fullStr | Exogenous hormones influence Brassica napus leaf cuticular wax deposition and cuticle function |
| title_full_unstemmed | Exogenous hormones influence Brassica napus leaf cuticular wax deposition and cuticle function |
| title_short | Exogenous hormones influence Brassica napus leaf cuticular wax deposition and cuticle function |
| title_sort | exogenous hormones influence brassica napus leaf cuticular wax deposition and cuticle function |
| topic | Cuticular wax Phytohormone Water loss rate Permeability Brassica napus |
| url | https://peerj.com/articles/9264.pdf |
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