Analysis of urine differential proteins in patients with allergic rhinitis
Background: Allergic rhinitis (AR) is one of the most common clinical allergic diseases. Early diagnosis and medical intervention will benefit patients with allergic rhinitis. In this study, we focused on changes in urine proteomics in AR patients to investigate their potential clinical utility in A...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023045310 |
| _version_ | 1797797173721563136 |
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| author | Na Liu Jitu Wang Xueyan Wang Man Zhang |
| author_facet | Na Liu Jitu Wang Xueyan Wang Man Zhang |
| author_sort | Na Liu |
| collection | DOAJ |
| description | Background: Allergic rhinitis (AR) is one of the most common clinical allergic diseases. Early diagnosis and medical intervention will benefit patients with allergic rhinitis. In this study, we focused on changes in urine proteomics in AR patients to investigate their potential clinical utility in AR diagnosis and evaluation. Material and methods: TMT-labeled mass spectrometry-based proteomics was carried out to identify differentially expressed proteins (DEPs) in urine between allergic rhinitis patients and normal control groups. The molecular biological role of DEPs was investigated by Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, and protein-protein interaction (PPI) network analysis. Results: Enrichment analysis showed that the differentially expressed proteins were mainly related to cell-cell adhesion, complement and coagulation cascades, peptidase activity regulation, MAP kinase activity, etc. Compared with the NC group, HLA-DRB1, WFDC12, and DEFA4, among the top ten up-regulated proteins in the urine of the AR group, were related to the biological process of the humoral immune response. Among the top 10 down-regulated proteins, GUSB, SQSTM1, and KIT are related to protein domain-specific binding in terms of molecular function. Conclusions: We found differential protein changes between AR patients and normal subjects may be related to the pathophysiological changes of AR, which provides the possibility for further exploration of urinary proteomics biomarkers in the future. |
| first_indexed | 2024-03-13T03:44:12Z |
| format | Article |
| id | doaj.art-0a2bc97a50c94a7f8459bd286d632301 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-13T03:44:12Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-0a2bc97a50c94a7f8459bd286d6323012023-06-23T04:43:19ZengElsevierHeliyon2405-84402023-06-0196e17323Analysis of urine differential proteins in patients with allergic rhinitisNa Liu0Jitu Wang1Xueyan Wang2Man Zhang3Clinical Laboratory Medicine, Beijing Shijitan Hospital, Capital Medical University, Beijing, China; Peking University Ninth School of Clinical Medicine, Beijing, China; Beijing Key Laboratory of Urinary Cellular Molecular Diagnostics, Beijing, ChinaClinical Laboratory Medicine, Beijing Shijitan Hospital, Capital Medical University, Beijing, China; Peking University Ninth School of Clinical Medicine, Beijing, China; Beijing Key Laboratory of Urinary Cellular Molecular Diagnostics, Beijing, ChinaDepartment of Allergy, Beijing Shijitan Hospital, Capital Medical University, Beijing, ChinaClinical Laboratory Medicine, Beijing Shijitan Hospital, Capital Medical University, Beijing, China; Peking University Ninth School of Clinical Medicine, Beijing, China; Beijing Key Laboratory of Urinary Cellular Molecular Diagnostics, Beijing, China; Corresponding author. Clinical Laboratory Medicine, Beijing Shijitan Hospital, Capital Medical University, Beijing, China.Background: Allergic rhinitis (AR) is one of the most common clinical allergic diseases. Early diagnosis and medical intervention will benefit patients with allergic rhinitis. In this study, we focused on changes in urine proteomics in AR patients to investigate their potential clinical utility in AR diagnosis and evaluation. Material and methods: TMT-labeled mass spectrometry-based proteomics was carried out to identify differentially expressed proteins (DEPs) in urine between allergic rhinitis patients and normal control groups. The molecular biological role of DEPs was investigated by Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, and protein-protein interaction (PPI) network analysis. Results: Enrichment analysis showed that the differentially expressed proteins were mainly related to cell-cell adhesion, complement and coagulation cascades, peptidase activity regulation, MAP kinase activity, etc. Compared with the NC group, HLA-DRB1, WFDC12, and DEFA4, among the top ten up-regulated proteins in the urine of the AR group, were related to the biological process of the humoral immune response. Among the top 10 down-regulated proteins, GUSB, SQSTM1, and KIT are related to protein domain-specific binding in terms of molecular function. Conclusions: We found differential protein changes between AR patients and normal subjects may be related to the pathophysiological changes of AR, which provides the possibility for further exploration of urinary proteomics biomarkers in the future.http://www.sciencedirect.com/science/article/pii/S2405844023045310Allergic rhinitis (AR)TMT-labeled mass spectrometryProteomics |
| spellingShingle | Na Liu Jitu Wang Xueyan Wang Man Zhang Analysis of urine differential proteins in patients with allergic rhinitis Heliyon Allergic rhinitis (AR) TMT-labeled mass spectrometry Proteomics |
| title | Analysis of urine differential proteins in patients with allergic rhinitis |
| title_full | Analysis of urine differential proteins in patients with allergic rhinitis |
| title_fullStr | Analysis of urine differential proteins in patients with allergic rhinitis |
| title_full_unstemmed | Analysis of urine differential proteins in patients with allergic rhinitis |
| title_short | Analysis of urine differential proteins in patients with allergic rhinitis |
| title_sort | analysis of urine differential proteins in patients with allergic rhinitis |
| topic | Allergic rhinitis (AR) TMT-labeled mass spectrometry Proteomics |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023045310 |
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