Isolation and Characterization of Thermophilic Enzymes Producing Microorganisms for Potential Therapeutic and Industrial Use
The need of extremophile enzymes is increased. Such enzymes had found their utility in bio-industries such as leather, food, animal feed, textiles, and in bioconversions and bioremediation. Screening of microorganisms producing enzymes from different areas of soil led to the isolation of 38 isolat...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2018-12-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/isolation-and-characterization-of-thermophilic-enzymes-producing-microorganisms-for-potential-therapeutic-and-industrial-use/ |
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| author | Hasnaa R. Temsaah Ahmed F. Azmy Mai Raslan Amr E. Ahmed Walaa G. Hozayen |
| author_facet | Hasnaa R. Temsaah Ahmed F. Azmy Mai Raslan Amr E. Ahmed Walaa G. Hozayen |
| author_sort | Hasnaa R. Temsaah |
| collection | DOAJ |
| description | The need of extremophile enzymes is increased. Such enzymes had found their utility in bio-industries such as
leather, food, animal feed, textiles, and in bioconversions and bioremediation. Screening of microorganisms
producing enzymes from different areas of soil led to the isolation of 38 isolates, the isolates were plate-screened
for their ability to produce extracellular enzymes. The promising strains were selected and screened for their
enzyme thermostability and screened quantitatively for potential industrial and therapeutic applications. Tolerance
of selected microorganisms was investigated to a varied range of pH, salinity, and enzyme activity over a range of
temperature. The genotypic identification of 16S rDNA sequence of the promising strains revealed that our strains
were Streptomyces mutabilis, Streptomyces ghanaensis, Streptomyces rochei and Enterobacter cloacae. The
isolated microorganisms quantified as an effective producer of industrially important enzymes amylase, cellulase,
esterase, casienase and therapeutic enzyme L-asparaginase. All enzymes produced from the four isolates show
enzyme activity and stability at different high temperature (60 °C, 80 °C, 100 °C). The amylase shows optimum
activity at 37 °C, while the other four enzymes show optimum activity at different high temperature (60 °C, 80
°C). The study shows that Streptomyces mutabilis produce acidophilic enzyme amylase, Streptomyces ghanaensis
produce acidophilic enzyme cellulase and neutrophilic enzyme esterase, Enterobacter cloacae and Streptomyces
rochei produce alkalophilic enzymes (L-asparaginase, caseinase) respectively. Enzymes show highest enzyme activity
at high NaCl concentration (5 and 7.5%). |
| first_indexed | 2024-12-18T01:00:07Z |
| format | Article |
| id | doaj.art-0a2d0564fc814a82b214c041161d2e02 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-18T01:00:07Z |
| publishDate | 2018-12-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-0a2d0564fc814a82b214c041161d2e022022-12-21T21:26:24ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2018-12-011241687170210.22207/JPAM.12.4.02Isolation and Characterization of Thermophilic Enzymes Producing Microorganisms for Potential Therapeutic and Industrial UseHasnaa R. Temsaah0Ahmed F. Azmy1Mai Raslan2Amr E. Ahmed3Walaa G. Hozayen41 Biotechnology and Life Sciences Department, Faculty of Postgraduate Studies for Advanced Sciences (PSAS), BeniSuef University, Egypt.2 Microbiology and Immunology Department. Faculty of Pharmacy, Beni-Suef University, Egypt.1 Biotechnology and Life Sciences Department, Faculty of Postgraduate Studies for Advanced Sciences (PSAS), BeniSuef University, Egypt.1 Biotechnology and Life Sciences Department, Faculty of Postgraduate Studies for Advanced Sciences (PSAS), BeniSuef University, Egypt.1 Biotechnology and Life Sciences Department, Faculty of Postgraduate Studies for Advanced Sciences (PSAS), BeniSuef University, Egypt. 3 Biochemistry Division, Chemistry Department, Faculty of Science, Beni-Suef University, Egypt.The need of extremophile enzymes is increased. Such enzymes had found their utility in bio-industries such as leather, food, animal feed, textiles, and in bioconversions and bioremediation. Screening of microorganisms producing enzymes from different areas of soil led to the isolation of 38 isolates, the isolates were plate-screened for their ability to produce extracellular enzymes. The promising strains were selected and screened for their enzyme thermostability and screened quantitatively for potential industrial and therapeutic applications. Tolerance of selected microorganisms was investigated to a varied range of pH, salinity, and enzyme activity over a range of temperature. The genotypic identification of 16S rDNA sequence of the promising strains revealed that our strains were Streptomyces mutabilis, Streptomyces ghanaensis, Streptomyces rochei and Enterobacter cloacae. The isolated microorganisms quantified as an effective producer of industrially important enzymes amylase, cellulase, esterase, casienase and therapeutic enzyme L-asparaginase. All enzymes produced from the four isolates show enzyme activity and stability at different high temperature (60 °C, 80 °C, 100 °C). The amylase shows optimum activity at 37 °C, while the other four enzymes show optimum activity at different high temperature (60 °C, 80 °C). The study shows that Streptomyces mutabilis produce acidophilic enzyme amylase, Streptomyces ghanaensis produce acidophilic enzyme cellulase and neutrophilic enzyme esterase, Enterobacter cloacae and Streptomyces rochei produce alkalophilic enzymes (L-asparaginase, caseinase) respectively. Enzymes show highest enzyme activity at high NaCl concentration (5 and 7.5%).https://microbiologyjournal.org/isolation-and-characterization-of-thermophilic-enzymes-producing-microorganisms-for-potential-therapeutic-and-industrial-use/microorganismthermophilic enzymesstreptomycesenterobacter |
| spellingShingle | Hasnaa R. Temsaah Ahmed F. Azmy Mai Raslan Amr E. Ahmed Walaa G. Hozayen Isolation and Characterization of Thermophilic Enzymes Producing Microorganisms for Potential Therapeutic and Industrial Use Journal of Pure and Applied Microbiology microorganism thermophilic enzymes streptomyces enterobacter |
| title | Isolation and Characterization of Thermophilic Enzymes Producing Microorganisms for Potential Therapeutic and Industrial Use |
| title_full | Isolation and Characterization of Thermophilic Enzymes Producing Microorganisms for Potential Therapeutic and Industrial Use |
| title_fullStr | Isolation and Characterization of Thermophilic Enzymes Producing Microorganisms for Potential Therapeutic and Industrial Use |
| title_full_unstemmed | Isolation and Characterization of Thermophilic Enzymes Producing Microorganisms for Potential Therapeutic and Industrial Use |
| title_short | Isolation and Characterization of Thermophilic Enzymes Producing Microorganisms for Potential Therapeutic and Industrial Use |
| title_sort | isolation and characterization of thermophilic enzymes producing microorganisms for potential therapeutic and industrial use |
| topic | microorganism thermophilic enzymes streptomyces enterobacter |
| url | https://microbiologyjournal.org/isolation-and-characterization-of-thermophilic-enzymes-producing-microorganisms-for-potential-therapeutic-and-industrial-use/ |
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