PARAQUAT TOLERANCE3 Is an E3 Ligase That Switches off Activated Oxidative Response by Targeting Histone-Modifying PROTEIN METHYLTRANSFERASE4b.
Oxidative stress is unavoidable for aerobic organisms. When abiotic and biotic stresses are encountered, oxidative damage could occur in cells. To avoid this damage, defense mechanisms must be timely and efficiently modulated. While the response to oxidative stress has been extensively studied in pl...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-09-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC5038976?pdf=render |
| _version_ | 1811273287712374784 |
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| author | Chao Luo Xiao-Teng Cai Jin Du Tao-Lan Zhao Peng-Fei Wang Ping-Xia Zhao Rui Liu Qi Xie Xiao-Feng Cao Cheng-Bin Xiang |
| author_facet | Chao Luo Xiao-Teng Cai Jin Du Tao-Lan Zhao Peng-Fei Wang Ping-Xia Zhao Rui Liu Qi Xie Xiao-Feng Cao Cheng-Bin Xiang |
| author_sort | Chao Luo |
| collection | DOAJ |
| description | Oxidative stress is unavoidable for aerobic organisms. When abiotic and biotic stresses are encountered, oxidative damage could occur in cells. To avoid this damage, defense mechanisms must be timely and efficiently modulated. While the response to oxidative stress has been extensively studied in plants, little is known about how the activated response is switched off when oxidative stress is diminished. By studying Arabidopsis mutant paraquat tolerance3, we identified the genetic locus PARAQUAT TOLERANCE3 (PQT3) as a major negative regulator of oxidative stress tolerance. PQT3, encoding an E3 ubiquitin ligase, is rapidly down-regulated by oxidative stress. PQT3 has E3 ubiquitin ligase activity in ubiquitination assay. Subsequently, we identified PRMT4b as a PQT3-interacting protein. By histone methylation, PRMT4b upregulates the expression of APX1 and GPX1, encoding two key enzymes against oxidative stress. On the other hand, PRMT4b is recognized by PQT3 for targeted degradation via 26S proteasome. Therefore, we have identified PQT3 as an E3 ligase that acts as a negative regulator of activated response to oxidative stress and found that histone modification by PRMT4b at APX1 and GPX1 loci plays an important role in oxidative stress tolerance. |
| first_indexed | 2024-04-12T22:56:25Z |
| format | Article |
| id | doaj.art-0a31cdeccdff4c07bcdeee309bbafc16 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-04-12T22:56:25Z |
| publishDate | 2016-09-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-0a31cdeccdff4c07bcdeee309bbafc162022-12-22T03:13:11ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042016-09-01129e100633210.1371/journal.pgen.1006332PARAQUAT TOLERANCE3 Is an E3 Ligase That Switches off Activated Oxidative Response by Targeting Histone-Modifying PROTEIN METHYLTRANSFERASE4b.Chao LuoXiao-Teng CaiJin DuTao-Lan ZhaoPeng-Fei WangPing-Xia ZhaoRui LiuQi XieXiao-Feng CaoCheng-Bin XiangOxidative stress is unavoidable for aerobic organisms. When abiotic and biotic stresses are encountered, oxidative damage could occur in cells. To avoid this damage, defense mechanisms must be timely and efficiently modulated. While the response to oxidative stress has been extensively studied in plants, little is known about how the activated response is switched off when oxidative stress is diminished. By studying Arabidopsis mutant paraquat tolerance3, we identified the genetic locus PARAQUAT TOLERANCE3 (PQT3) as a major negative regulator of oxidative stress tolerance. PQT3, encoding an E3 ubiquitin ligase, is rapidly down-regulated by oxidative stress. PQT3 has E3 ubiquitin ligase activity in ubiquitination assay. Subsequently, we identified PRMT4b as a PQT3-interacting protein. By histone methylation, PRMT4b upregulates the expression of APX1 and GPX1, encoding two key enzymes against oxidative stress. On the other hand, PRMT4b is recognized by PQT3 for targeted degradation via 26S proteasome. Therefore, we have identified PQT3 as an E3 ligase that acts as a negative regulator of activated response to oxidative stress and found that histone modification by PRMT4b at APX1 and GPX1 loci plays an important role in oxidative stress tolerance.http://europepmc.org/articles/PMC5038976?pdf=render |
| spellingShingle | Chao Luo Xiao-Teng Cai Jin Du Tao-Lan Zhao Peng-Fei Wang Ping-Xia Zhao Rui Liu Qi Xie Xiao-Feng Cao Cheng-Bin Xiang PARAQUAT TOLERANCE3 Is an E3 Ligase That Switches off Activated Oxidative Response by Targeting Histone-Modifying PROTEIN METHYLTRANSFERASE4b. PLoS Genetics |
| title | PARAQUAT TOLERANCE3 Is an E3 Ligase That Switches off Activated Oxidative Response by Targeting Histone-Modifying PROTEIN METHYLTRANSFERASE4b. |
| title_full | PARAQUAT TOLERANCE3 Is an E3 Ligase That Switches off Activated Oxidative Response by Targeting Histone-Modifying PROTEIN METHYLTRANSFERASE4b. |
| title_fullStr | PARAQUAT TOLERANCE3 Is an E3 Ligase That Switches off Activated Oxidative Response by Targeting Histone-Modifying PROTEIN METHYLTRANSFERASE4b. |
| title_full_unstemmed | PARAQUAT TOLERANCE3 Is an E3 Ligase That Switches off Activated Oxidative Response by Targeting Histone-Modifying PROTEIN METHYLTRANSFERASE4b. |
| title_short | PARAQUAT TOLERANCE3 Is an E3 Ligase That Switches off Activated Oxidative Response by Targeting Histone-Modifying PROTEIN METHYLTRANSFERASE4b. |
| title_sort | paraquat tolerance3 is an e3 ligase that switches off activated oxidative response by targeting histone modifying protein methyltransferase4b |
| url | http://europepmc.org/articles/PMC5038976?pdf=render |
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