Molecular Imprinted Based Quartz Crystal Microbalance Sensors for Bacteria and Spores
A molecular imprinting strategy was combined with mass-sensitive transducers to generate robust and reliable biomimetic sensor systems for the detection of bioparticles. The patterning of polymers with bioanalytes enabled us to detect <i>Escherichia coli</i> (<i>E. coli</i>)...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/8/3/64 |
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| author | Usman Latif Serpil Can Hermann F. Sussitz Franz L. Dickert |
| author_facet | Usman Latif Serpil Can Hermann F. Sussitz Franz L. Dickert |
| author_sort | Usman Latif |
| collection | DOAJ |
| description | A molecular imprinting strategy was combined with mass-sensitive transducers to generate robust and reliable biomimetic sensor systems for the detection of bioparticles. The patterning of polymers with bioanalytes enabled us to detect <i>Escherichia coli</i> (<i>E. coli</i>) bacteria with quartz crystal microbalance (QCM). The QCM sensor results were compared with direct Atomic Force Microscopy (AFM) measurements—bacteria cells adhering to the sensor coatings were counted. The recognition sites generated by <i>Bacillus subtilis</i> (<i>B. subtilis</i>) spores could successfully and reversibly recognize the template analyte and ensured rapid sensing. Cross sensitive measurements clearly showed the advantage of the molecular imprinting strategy, by which spores of <i>Bacillus</i> species (<i>subtilis</i> and <i>thuringiensis</i>) could easily be differentiated and selectively detected. The growth of <i>B. subtilis</i> from its spores was observed at 42 °C in appropriate nutrient solution of glucose and ammonium sulfate over a period of 15 h. Moreover, the growth of <i>B. subtilis</i> bacteria from its respective spores was studied by increasing the glucose concentration until saturation effect of the sensor. The polymeric sensor coatings were patterned to fix the <i>B. subtilis</i> in order to investigate osmotic effects according to a frequency response of 400 Hz by altering the ionic strength of 0.1 M. |
| first_indexed | 2024-03-10T17:58:39Z |
| format | Article |
| id | doaj.art-0a34c9bb1d2b4b4290cf917d3dcc1490 |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-10T17:58:39Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-0a34c9bb1d2b4b4290cf917d3dcc14902023-11-20T09:03:58ZengMDPI AGChemosensors2227-90402020-08-01836410.3390/chemosensors8030064Molecular Imprinted Based Quartz Crystal Microbalance Sensors for Bacteria and SporesUsman Latif0Serpil Can1Hermann F. Sussitz2Franz L. Dickert3Department of Analytical Chemistry, University of Vienna, Waehringer Str. 38, A-1090 Wien, AustriaDepartment of Analytical Chemistry, University of Vienna, Waehringer Str. 38, A-1090 Wien, AustriaDepartment of Analytical Chemistry, University of Vienna, Waehringer Str. 38, A-1090 Wien, AustriaDepartment of Analytical Chemistry, University of Vienna, Waehringer Str. 38, A-1090 Wien, AustriaA molecular imprinting strategy was combined with mass-sensitive transducers to generate robust and reliable biomimetic sensor systems for the detection of bioparticles. The patterning of polymers with bioanalytes enabled us to detect <i>Escherichia coli</i> (<i>E. coli</i>) bacteria with quartz crystal microbalance (QCM). The QCM sensor results were compared with direct Atomic Force Microscopy (AFM) measurements—bacteria cells adhering to the sensor coatings were counted. The recognition sites generated by <i>Bacillus subtilis</i> (<i>B. subtilis</i>) spores could successfully and reversibly recognize the template analyte and ensured rapid sensing. Cross sensitive measurements clearly showed the advantage of the molecular imprinting strategy, by which spores of <i>Bacillus</i> species (<i>subtilis</i> and <i>thuringiensis</i>) could easily be differentiated and selectively detected. The growth of <i>B. subtilis</i> from its spores was observed at 42 °C in appropriate nutrient solution of glucose and ammonium sulfate over a period of 15 h. Moreover, the growth of <i>B. subtilis</i> bacteria from its respective spores was studied by increasing the glucose concentration until saturation effect of the sensor. The polymeric sensor coatings were patterned to fix the <i>B. subtilis</i> in order to investigate osmotic effects according to a frequency response of 400 Hz by altering the ionic strength of 0.1 M.https://www.mdpi.com/2227-9040/8/3/64monitoring cells and sporesmolecular imprintingQCM sensorsAFM |
| spellingShingle | Usman Latif Serpil Can Hermann F. Sussitz Franz L. Dickert Molecular Imprinted Based Quartz Crystal Microbalance Sensors for Bacteria and Spores Chemosensors monitoring cells and spores molecular imprinting QCM sensors AFM |
| title | Molecular Imprinted Based Quartz Crystal Microbalance Sensors for Bacteria and Spores |
| title_full | Molecular Imprinted Based Quartz Crystal Microbalance Sensors for Bacteria and Spores |
| title_fullStr | Molecular Imprinted Based Quartz Crystal Microbalance Sensors for Bacteria and Spores |
| title_full_unstemmed | Molecular Imprinted Based Quartz Crystal Microbalance Sensors for Bacteria and Spores |
| title_short | Molecular Imprinted Based Quartz Crystal Microbalance Sensors for Bacteria and Spores |
| title_sort | molecular imprinted based quartz crystal microbalance sensors for bacteria and spores |
| topic | monitoring cells and spores molecular imprinting QCM sensors AFM |
| url | https://www.mdpi.com/2227-9040/8/3/64 |
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