Blockade of the Naloxone-induced Aversion in Morphine-conditioned Wistar Rats by L-Arginine Intra-central Amygdala
AbstractObjective(s)Single injection of naloxone, a selective antagonist of morphine, prior to the drug conditioning testing was used to investigate on morphine dependence.Materials and MethodsConditioning to morphine (2.5-10 mg/kg, s.c.) was established in adult male Wistar rats (weighing...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2011-03-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://www.mums.ac.ir/shares/basic_medical/basicmedjou/2011/mar/a10.pdf |
| _version_ | 1811257847664607232 |
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| author | Mahnaz Rahimpour Manizheh Karami Sara Karimi Abbas Haghparast Mohammad Reza Jalali Farzaneh Sabouni |
| author_facet | Mahnaz Rahimpour Manizheh Karami Sara Karimi Abbas Haghparast Mohammad Reza Jalali Farzaneh Sabouni |
| author_sort | Mahnaz Rahimpour |
| collection | DOAJ |
| description | AbstractObjective(s)Single injection of naloxone, a selective antagonist of morphine, prior to the drug conditioning testing was used to investigate on morphine dependence.Materials and MethodsConditioning to morphine (2.5-10 mg/kg, s.c.) was established in adult male Wistar rats (weighing 200-250 g) using an unbiased procedure. Nitric oxide agents were microinjected into the central amygdala prior to naloxone-paired place conditioning testing.ResultsThe results showed that morphine produced a significant dose-dependent place preference in animals. Naloxone (0.1-0.4 mg/kg, i.p.) injections pre-testing of the response to morphine (7.5 mg/kg, s.c.) caused a significant aversion at the higher doses (0.4 mg/kg, i.p.). This response was reversed by microinjection of L-arginine (0.3-3 µg/rat, intra-central amygdala) prior to naloxone on the day of the testing. The response to L-arginine was blocked by pre-injection of NG-nitro-L-arginine methyl ester (L-NAME) (intra-central amygdala).ConclusionA single injection of naloxone on the test day of morphine place conditioning may simply reveal the occurrence of morphine dependence in rats, and that the nitric oxide in the central amygdala most likely plays a key role in this phenomenon. |
| first_indexed | 2024-04-12T18:03:56Z |
| format | Article |
| id | doaj.art-0a355e849c204a03a85338c31729cecf |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-12T18:03:56Z |
| publishDate | 2011-03-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-0a355e849c204a03a85338c31729cecf2022-12-22T03:22:03ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742011-03-011449Blockade of the Naloxone-induced Aversion in Morphine-conditioned Wistar Rats by L-Arginine Intra-central AmygdalaMahnaz RahimpourManizheh KaramiSara KarimiAbbas HaghparastMohammad Reza JalaliFarzaneh SabouniAbstractObjective(s)Single injection of naloxone, a selective antagonist of morphine, prior to the drug conditioning testing was used to investigate on morphine dependence.Materials and MethodsConditioning to morphine (2.5-10 mg/kg, s.c.) was established in adult male Wistar rats (weighing 200-250 g) using an unbiased procedure. Nitric oxide agents were microinjected into the central amygdala prior to naloxone-paired place conditioning testing.ResultsThe results showed that morphine produced a significant dose-dependent place preference in animals. Naloxone (0.1-0.4 mg/kg, i.p.) injections pre-testing of the response to morphine (7.5 mg/kg, s.c.) caused a significant aversion at the higher doses (0.4 mg/kg, i.p.). This response was reversed by microinjection of L-arginine (0.3-3 µg/rat, intra-central amygdala) prior to naloxone on the day of the testing. The response to L-arginine was blocked by pre-injection of NG-nitro-L-arginine methyl ester (L-NAME) (intra-central amygdala).ConclusionA single injection of naloxone on the test day of morphine place conditioning may simply reveal the occurrence of morphine dependence in rats, and that the nitric oxide in the central amygdala most likely plays a key role in this phenomenon.http://www.mums.ac.ir/shares/basic_medical/basicmedjou/2011/mar/a10.pdfAmygdalaMorphine dependenceNaloxoneNitric oxide |
| spellingShingle | Mahnaz Rahimpour Manizheh Karami Sara Karimi Abbas Haghparast Mohammad Reza Jalali Farzaneh Sabouni Blockade of the Naloxone-induced Aversion in Morphine-conditioned Wistar Rats by L-Arginine Intra-central Amygdala Iranian Journal of Basic Medical Sciences Amygdala Morphine dependence Naloxone Nitric oxide |
| title | Blockade of the Naloxone-induced Aversion in Morphine-conditioned Wistar Rats by L-Arginine Intra-central Amygdala |
| title_full | Blockade of the Naloxone-induced Aversion in Morphine-conditioned Wistar Rats by L-Arginine Intra-central Amygdala |
| title_fullStr | Blockade of the Naloxone-induced Aversion in Morphine-conditioned Wistar Rats by L-Arginine Intra-central Amygdala |
| title_full_unstemmed | Blockade of the Naloxone-induced Aversion in Morphine-conditioned Wistar Rats by L-Arginine Intra-central Amygdala |
| title_short | Blockade of the Naloxone-induced Aversion in Morphine-conditioned Wistar Rats by L-Arginine Intra-central Amygdala |
| title_sort | blockade of the naloxone induced aversion in morphine conditioned wistar rats by l arginine intra central amygdala |
| topic | Amygdala Morphine dependence Naloxone Nitric oxide |
| url | http://www.mums.ac.ir/shares/basic_medical/basicmedjou/2011/mar/a10.pdf |
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