Investigation on Potential ESKAPE Surrogates for 222 and 254 nm Irradiation Experiments
BackgroundDue to the increase in multidrug-resistant pathogens, it is important to investigate further antimicrobial options. In order not to have to work directly with pathogens, the investigation of possible surrogates is an important aspect. It is examined how suitable possible surrogate candidat...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-07-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.942708/full |
| _version_ | 1829106898913198080 |
|---|---|
| author | Anna-Maria Gierke Martin Hessling |
| author_facet | Anna-Maria Gierke Martin Hessling |
| author_sort | Anna-Maria Gierke |
| collection | DOAJ |
| description | BackgroundDue to the increase in multidrug-resistant pathogens, it is important to investigate further antimicrobial options. In order not to have to work directly with pathogens, the investigation of possible surrogates is an important aspect. It is examined how suitable possible surrogate candidates for ESKAPE pathogens are for UVC applications. In addition, the inactivation sensitivities to 222 and 254 nm radiation are compared in relation.MethodsNon-pathogenic members (Enterococcus mundtii, Staphylococcus carnosus, Acinetobacter kookii, Pseudomonas fluorescens and Escherichia coli) of genera of ESKAPE strains were photoinactivated in PBS with irradiation wavelengths of 222 and 254 nm (no non-pathogenic Klebsiella was available). Log reduction doses were determined and compared to published photoinactivation results on ESKAPE pathogens. It was assumed that non-pathogenic bacteria could be designated as surrogates for one wavelength and one ESKAPE strain, if the doses were between the 25 and 75% quantiles of published log reduction dose of the corresponding pathogen.ResultsFor all non-pathogen relatives (except A. kookii), higher average log reduction doses were required for irradiation at 222 nm than at 254 nm. Comparison by boxplot revealed that five of eight determined log reduction doses of the possible surrogates were within the 25 and 75% quantiles of the data for ESKAPE pathogens. The measured log reduction dose for non-pathogenic E. coli was above the 75% quantile at 222 nm, and the log reduction dose for S. carnosus was below the 25% quantile at 254 nm.ConclusionFor more than half of the studied cases, the examined ESKAPE relatives in this study can be applied as surrogates for ESKAPE pathogens. Because of lack of data, no clear statement could be made for Enterococcus faecalis at 222 nm and Acinetobacter baumannii at both wavelengths. |
| first_indexed | 2024-12-12T06:29:35Z |
| format | Article |
| id | doaj.art-0a3785653f8941a588a083855f81812a |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T06:29:35Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-0a3785653f8941a588a083855f81812a2022-12-22T00:34:37ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-07-011310.3389/fmicb.2022.942708942708Investigation on Potential ESKAPE Surrogates for 222 and 254 nm Irradiation ExperimentsAnna-Maria GierkeMartin HesslingBackgroundDue to the increase in multidrug-resistant pathogens, it is important to investigate further antimicrobial options. In order not to have to work directly with pathogens, the investigation of possible surrogates is an important aspect. It is examined how suitable possible surrogate candidates for ESKAPE pathogens are for UVC applications. In addition, the inactivation sensitivities to 222 and 254 nm radiation are compared in relation.MethodsNon-pathogenic members (Enterococcus mundtii, Staphylococcus carnosus, Acinetobacter kookii, Pseudomonas fluorescens and Escherichia coli) of genera of ESKAPE strains were photoinactivated in PBS with irradiation wavelengths of 222 and 254 nm (no non-pathogenic Klebsiella was available). Log reduction doses were determined and compared to published photoinactivation results on ESKAPE pathogens. It was assumed that non-pathogenic bacteria could be designated as surrogates for one wavelength and one ESKAPE strain, if the doses were between the 25 and 75% quantiles of published log reduction dose of the corresponding pathogen.ResultsFor all non-pathogen relatives (except A. kookii), higher average log reduction doses were required for irradiation at 222 nm than at 254 nm. Comparison by boxplot revealed that five of eight determined log reduction doses of the possible surrogates were within the 25 and 75% quantiles of the data for ESKAPE pathogens. The measured log reduction dose for non-pathogenic E. coli was above the 75% quantile at 222 nm, and the log reduction dose for S. carnosus was below the 25% quantile at 254 nm.ConclusionFor more than half of the studied cases, the examined ESKAPE relatives in this study can be applied as surrogates for ESKAPE pathogens. Because of lack of data, no clear statement could be made for Enterococcus faecalis at 222 nm and Acinetobacter baumannii at both wavelengths.https://www.frontiersin.org/articles/10.3389/fmicb.2022.942708/fulldisinfectionESKAPE pathogensurrogatesfar-UVCphotoinactivationradiation |
| spellingShingle | Anna-Maria Gierke Martin Hessling Investigation on Potential ESKAPE Surrogates for 222 and 254 nm Irradiation Experiments Frontiers in Microbiology disinfection ESKAPE pathogen surrogates far-UVC photoinactivation radiation |
| title | Investigation on Potential ESKAPE Surrogates for 222 and 254 nm Irradiation Experiments |
| title_full | Investigation on Potential ESKAPE Surrogates for 222 and 254 nm Irradiation Experiments |
| title_fullStr | Investigation on Potential ESKAPE Surrogates for 222 and 254 nm Irradiation Experiments |
| title_full_unstemmed | Investigation on Potential ESKAPE Surrogates for 222 and 254 nm Irradiation Experiments |
| title_short | Investigation on Potential ESKAPE Surrogates for 222 and 254 nm Irradiation Experiments |
| title_sort | investigation on potential eskape surrogates for 222 and 254 nm irradiation experiments |
| topic | disinfection ESKAPE pathogen surrogates far-UVC photoinactivation radiation |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.942708/full |
| work_keys_str_mv | AT annamariagierke investigationonpotentialeskapesurrogatesfor222and254nmirradiationexperiments AT martinhessling investigationonpotentialeskapesurrogatesfor222and254nmirradiationexperiments |