Synthesis of gold nanoparticles using Sambucus wightiana extract and investigation of its antimicrobial, anti-inflammatory, antioxidant and analgesic activities
Resistance to antimicrobial agents are rendering therapies ineffective around the globe, leading to increased mortality and treatment cost. Likewise, non-steroidal anti-inflammatory drugs (NSAIDs) and analgesics possess several side effects particularly peptic ulcer and gastrointestinal problems. Me...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-10-01
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| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535221003580 |
| _version_ | 1819082275117072384 |
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| author | Fazli Khuda Zafar Ul Haq Ihsan Ilahi Rahim Ullah Ayub Khan Hassan Fouad Atif Ali Khan Khalil Zaki Ullah Muhammad Umar Khayam Sahibzada Yasar Shah Muhammad Abbas Tayyaba Iftikhar Gaber El-Saber Batiha |
| author_facet | Fazli Khuda Zafar Ul Haq Ihsan Ilahi Rahim Ullah Ayub Khan Hassan Fouad Atif Ali Khan Khalil Zaki Ullah Muhammad Umar Khayam Sahibzada Yasar Shah Muhammad Abbas Tayyaba Iftikhar Gaber El-Saber Batiha |
| author_sort | Fazli Khuda |
| collection | DOAJ |
| description | Resistance to antimicrobial agents are rendering therapies ineffective around the globe, leading to increased mortality and treatment cost. Likewise, non-steroidal anti-inflammatory drugs (NSAIDs) and analgesics possess several side effects particularly peptic ulcer and gastrointestinal problems. Metallic nanoparticles significantly enhances the therapeutic efficacy of natural extracts owing to improved bioavailability thereby lowering the dose and side effects. The purpose of this research was to investigate the efficacy of gold nanoparticles (AuNPs). In this study, Sambucus wightiana whole plant aqueous extract was used for rapid and eco-friendly synthesis of AuNPs. They were characterized by various analytical techniques including UV–Visible spectroscopy (UV–Vis), transmission and scanning electron microscopy (TEM and SEM), energy dispersive X-ray spectroscopy (EDX), Zetasizer, X-ray diffractometer (XRD) and Fourier transform infra-red spectroscopy (FTIR). The UV–Vis spectra revealed a distinct absorption peak at 539 nm; TEM and SEM images confirmed the formation of heterogeneously dispersed AuNPs with an average area of 152.77 nm2 and width of 15.96 nm. The AuNPs showed significant inhibitory zones against Escherichia coli (25 mm), Staphylococcus epidermis (23 mm) and Salmonella enteritidis (18 mm) with MIC values 0.13, 0.11 and 0.16 mg/ml, respectively. Among fungal strains it showed highest percent inhibition against Fusarium solani (90%) and Microsporum canis (80%) with MIC values 0.02 and 0.01 mg/ml, respectively. It showed maximum anti-inflammatory activity (43.70, 48.80 and 57.08%) at 20 mg/kg dose at both early and late hours of inflammation. Likewise, in vitro models depicted concentration dependent inhibition of 5-LOX and COX-2 enzymes. AuNPs showed highest antioxidant activity (68.7% inhibition) at 1000 µg/ml, compared to ascorbic acid that showed 77.8% inhibition at the same concentration. Similarly, it exhibited significant (P ≤ 0.001) dose dependent analgesic effect with maximum inhibition (56.22%) at 20 mg/kg. In conclusion, the above findings suggest that AuNPs should be studied further in order to develop safe and effective formulations. |
| first_indexed | 2024-12-21T20:14:05Z |
| format | Article |
| id | doaj.art-0a3880d27a3c42768c507eacf748c9bb |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-12-21T20:14:05Z |
| publishDate | 2021-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-0a3880d27a3c42768c507eacf748c9bb2022-12-21T18:51:40ZengElsevierArabian Journal of Chemistry1878-53522021-10-011410103343Synthesis of gold nanoparticles using Sambucus wightiana extract and investigation of its antimicrobial, anti-inflammatory, antioxidant and analgesic activitiesFazli Khuda0Zafar Ul Haq1Ihsan Ilahi2Rahim Ullah3Ayub Khan4Hassan Fouad5Atif Ali Khan Khalil6Zaki Ullah7Muhammad Umar Khayam Sahibzada8Yasar Shah9Muhammad Abbas10Tayyaba Iftikhar11Gaber El-Saber Batiha12Department of Pharmacy, University of Peshawar, Peshawar, Pakistan; Corresponding author.Department of Pharmacy, University of Peshawar, Peshawar, PakistanDepartment of Pharmacy, University of Peshawar, Peshawar, PakistanDepartment of Pharmacy, University of Peshawar, Peshawar, PakistanDepartment of Chemistry, University of Education, Jauharabad Campus, Lahore, PakistanApplied Medical Science Department, Community College, King Saud University, Riyadh, Saudi ArabiaDepartment of Biological Sciences, National University of Medical Sciences, Rawalpindi, Pakistan; College of Pharmacy and Research Institute of Pharmaceutical Sciences, Gyeongsang National University, Jinju, KoreaDepartment of Pharmacy, University of Peshawar, Peshawar, PakistanDepartment of Pharmacy, Sarhad University of Science and Information Technology, Peshawar, PakistanDepartment of Pharmacy, Abdul Wali Khan University, Mardan, PakistanDepartment of Pharmacy, Abdul Wali Khan University, Mardan, PakistanDepartment of Pharmacy, Abdul Wali Khan University, Mardan, PakistanDepartment of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour, AlBeheira, EgyptResistance to antimicrobial agents are rendering therapies ineffective around the globe, leading to increased mortality and treatment cost. Likewise, non-steroidal anti-inflammatory drugs (NSAIDs) and analgesics possess several side effects particularly peptic ulcer and gastrointestinal problems. Metallic nanoparticles significantly enhances the therapeutic efficacy of natural extracts owing to improved bioavailability thereby lowering the dose and side effects. The purpose of this research was to investigate the efficacy of gold nanoparticles (AuNPs). In this study, Sambucus wightiana whole plant aqueous extract was used for rapid and eco-friendly synthesis of AuNPs. They were characterized by various analytical techniques including UV–Visible spectroscopy (UV–Vis), transmission and scanning electron microscopy (TEM and SEM), energy dispersive X-ray spectroscopy (EDX), Zetasizer, X-ray diffractometer (XRD) and Fourier transform infra-red spectroscopy (FTIR). The UV–Vis spectra revealed a distinct absorption peak at 539 nm; TEM and SEM images confirmed the formation of heterogeneously dispersed AuNPs with an average area of 152.77 nm2 and width of 15.96 nm. The AuNPs showed significant inhibitory zones against Escherichia coli (25 mm), Staphylococcus epidermis (23 mm) and Salmonella enteritidis (18 mm) with MIC values 0.13, 0.11 and 0.16 mg/ml, respectively. Among fungal strains it showed highest percent inhibition against Fusarium solani (90%) and Microsporum canis (80%) with MIC values 0.02 and 0.01 mg/ml, respectively. It showed maximum anti-inflammatory activity (43.70, 48.80 and 57.08%) at 20 mg/kg dose at both early and late hours of inflammation. Likewise, in vitro models depicted concentration dependent inhibition of 5-LOX and COX-2 enzymes. AuNPs showed highest antioxidant activity (68.7% inhibition) at 1000 µg/ml, compared to ascorbic acid that showed 77.8% inhibition at the same concentration. Similarly, it exhibited significant (P ≤ 0.001) dose dependent analgesic effect with maximum inhibition (56.22%) at 20 mg/kg. In conclusion, the above findings suggest that AuNPs should be studied further in order to develop safe and effective formulations.http://www.sciencedirect.com/science/article/pii/S1878535221003580Sambucus wightianaGold nanoparticlesCharacterizationBiological screening |
| spellingShingle | Fazli Khuda Zafar Ul Haq Ihsan Ilahi Rahim Ullah Ayub Khan Hassan Fouad Atif Ali Khan Khalil Zaki Ullah Muhammad Umar Khayam Sahibzada Yasar Shah Muhammad Abbas Tayyaba Iftikhar Gaber El-Saber Batiha Synthesis of gold nanoparticles using Sambucus wightiana extract and investigation of its antimicrobial, anti-inflammatory, antioxidant and analgesic activities Arabian Journal of Chemistry Sambucus wightiana Gold nanoparticles Characterization Biological screening |
| title | Synthesis of gold nanoparticles using Sambucus wightiana extract and investigation of its antimicrobial, anti-inflammatory, antioxidant and analgesic activities |
| title_full | Synthesis of gold nanoparticles using Sambucus wightiana extract and investigation of its antimicrobial, anti-inflammatory, antioxidant and analgesic activities |
| title_fullStr | Synthesis of gold nanoparticles using Sambucus wightiana extract and investigation of its antimicrobial, anti-inflammatory, antioxidant and analgesic activities |
| title_full_unstemmed | Synthesis of gold nanoparticles using Sambucus wightiana extract and investigation of its antimicrobial, anti-inflammatory, antioxidant and analgesic activities |
| title_short | Synthesis of gold nanoparticles using Sambucus wightiana extract and investigation of its antimicrobial, anti-inflammatory, antioxidant and analgesic activities |
| title_sort | synthesis of gold nanoparticles using sambucus wightiana extract and investigation of its antimicrobial anti inflammatory antioxidant and analgesic activities |
| topic | Sambucus wightiana Gold nanoparticles Characterization Biological screening |
| url | http://www.sciencedirect.com/science/article/pii/S1878535221003580 |
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