Quantitative label-free proteomic analysis of excretory-secretory proteins in different developmental stages of Trichinella spiralis
Abstract Trichinella spiralis (T. spiralis) is a zoonotic parasitic nematode with a unique life cycle, as all developmental stages are contained within a single host. Excretory-secretory (ES) proteins are the main targets of the interactions between T. spiralis and the host at different stages of de...
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| Format: | Article |
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BMC
2024-01-01
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| Series: | Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s13567-023-01258-7 |
| _version_ | 1797362979319054336 |
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| author | Yadong Liu Juncheng Liu Nan Wang Xihuo You Yaming Yang Jing Ding Xiaolei Liu Mingyuan Liu Chen Li Ning Xu |
| author_facet | Yadong Liu Juncheng Liu Nan Wang Xihuo You Yaming Yang Jing Ding Xiaolei Liu Mingyuan Liu Chen Li Ning Xu |
| author_sort | Yadong Liu |
| collection | DOAJ |
| description | Abstract Trichinella spiralis (T. spiralis) is a zoonotic parasitic nematode with a unique life cycle, as all developmental stages are contained within a single host. Excretory-secretory (ES) proteins are the main targets of the interactions between T. spiralis and the host at different stages of development and are essential for parasite survival. However, the ES protein profiles of T. spiralis at different developmental stages have not been characterized. The proteomes of ES proteins from different developmental stages, namely, muscle larvae (ML), intestinal infective larvae (IIL), preadult (PA) 6 h, PA 30 h, adult (Ad) 3 days post-infection (dpi) and Ad 6 dpi, were characterized via label-free mass spectrometry analysis in combination with bioinformatics. A total of 1217 proteins were identified from 9341 unique peptides in all developmental stages, 590 of which were quantified and differentially expressed. GO classification and KEGG pathway analysis revealed that these proteins were important for the growth of the larvae and involved in energy metabolism. Moreover, the heat shock cognate 71 kDa protein was the centre of protein interactions at different developmental stages. The results of this study provide comprehensive proteomic data on ES proteins and reveal that these ES proteins were differentially expressed at different developmental stages. Differential proteins are associated with parasite survival and the host immune response and may be potential early diagnostic antigen or antiparasitic vaccine candidates. |
| first_indexed | 2024-03-08T16:14:55Z |
| format | Article |
| id | doaj.art-0a3a3cd6b790444f854b21df28f27f75 |
| institution | Directory Open Access Journal |
| issn | 1297-9716 |
| language | English |
| last_indexed | 2024-03-08T16:14:55Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-0a3a3cd6b790444f854b21df28f27f752024-01-07T12:39:41ZengBMCVeterinary Research1297-97162024-01-0155111810.1186/s13567-023-01258-7Quantitative label-free proteomic analysis of excretory-secretory proteins in different developmental stages of Trichinella spiralisYadong Liu0Juncheng Liu1Nan Wang2Xihuo You3Yaming Yang4Jing Ding5Xiaolei Liu6Mingyuan Liu7Chen Li8Ning Xu9State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin UniversityCollege of Veterinary Medicine, Shandong Agricultural UniversityJilin Agricultural UniversityBeijing Agrichina Pharmaceutical Co., Ltd.Yunnan Institute of Parasitic DiseasesState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin UniversityState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin UniversityState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin UniversityState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin UniversityState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin UniversityAbstract Trichinella spiralis (T. spiralis) is a zoonotic parasitic nematode with a unique life cycle, as all developmental stages are contained within a single host. Excretory-secretory (ES) proteins are the main targets of the interactions between T. spiralis and the host at different stages of development and are essential for parasite survival. However, the ES protein profiles of T. spiralis at different developmental stages have not been characterized. The proteomes of ES proteins from different developmental stages, namely, muscle larvae (ML), intestinal infective larvae (IIL), preadult (PA) 6 h, PA 30 h, adult (Ad) 3 days post-infection (dpi) and Ad 6 dpi, were characterized via label-free mass spectrometry analysis in combination with bioinformatics. A total of 1217 proteins were identified from 9341 unique peptides in all developmental stages, 590 of which were quantified and differentially expressed. GO classification and KEGG pathway analysis revealed that these proteins were important for the growth of the larvae and involved in energy metabolism. Moreover, the heat shock cognate 71 kDa protein was the centre of protein interactions at different developmental stages. The results of this study provide comprehensive proteomic data on ES proteins and reveal that these ES proteins were differentially expressed at different developmental stages. Differential proteins are associated with parasite survival and the host immune response and may be potential early diagnostic antigen or antiparasitic vaccine candidates.https://doi.org/10.1186/s13567-023-01258-7Trichinella spiralisexcretory-secretory proteinslabel-freeproteomicsLC‒MS/MS |
| spellingShingle | Yadong Liu Juncheng Liu Nan Wang Xihuo You Yaming Yang Jing Ding Xiaolei Liu Mingyuan Liu Chen Li Ning Xu Quantitative label-free proteomic analysis of excretory-secretory proteins in different developmental stages of Trichinella spiralis Veterinary Research Trichinella spiralis excretory-secretory proteins label-free proteomics LC‒MS/MS |
| title | Quantitative label-free proteomic analysis of excretory-secretory proteins in different developmental stages of Trichinella spiralis |
| title_full | Quantitative label-free proteomic analysis of excretory-secretory proteins in different developmental stages of Trichinella spiralis |
| title_fullStr | Quantitative label-free proteomic analysis of excretory-secretory proteins in different developmental stages of Trichinella spiralis |
| title_full_unstemmed | Quantitative label-free proteomic analysis of excretory-secretory proteins in different developmental stages of Trichinella spiralis |
| title_short | Quantitative label-free proteomic analysis of excretory-secretory proteins in different developmental stages of Trichinella spiralis |
| title_sort | quantitative label free proteomic analysis of excretory secretory proteins in different developmental stages of trichinella spiralis |
| topic | Trichinella spiralis excretory-secretory proteins label-free proteomics LC‒MS/MS |
| url | https://doi.org/10.1186/s13567-023-01258-7 |
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