Evaluating the effect of spaceflight on the host–pathogen interaction between human intestinal epithelial cells and Salmonella Typhimurium
Abstract Spaceflight uniquely alters the physiology of both human cells and microbial pathogens, stimulating cellular and molecular changes directly relevant to infectious disease. However, the influence of this environment on host–pathogen interactions remains poorly understood. Here we report our...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-03-01
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| Series: | npj Microgravity |
| Online Access: | https://doi.org/10.1038/s41526-021-00136-w |
| _version_ | 1797641524386725888 |
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| author | Jennifer Barrila Shameema F. Sarker Nicole Hansmeier Shanshan Yang Kristina Buss Natalia Briones Jin Park Richard R. Davis Rebecca J. Forsyth C. Mark Ott Kevin Sato Cristine Kosnik Anthony Yang Cheryl Shimoda Nicole Rayl Diana Ly Aaron Landenberger Stephanie D. Wilson Naoko Yamazaki Jason Steel Camila Montano Rolf U. Halden Tom Cannon Sarah L. Castro-Wallace Cheryl A. Nickerson |
| author_facet | Jennifer Barrila Shameema F. Sarker Nicole Hansmeier Shanshan Yang Kristina Buss Natalia Briones Jin Park Richard R. Davis Rebecca J. Forsyth C. Mark Ott Kevin Sato Cristine Kosnik Anthony Yang Cheryl Shimoda Nicole Rayl Diana Ly Aaron Landenberger Stephanie D. Wilson Naoko Yamazaki Jason Steel Camila Montano Rolf U. Halden Tom Cannon Sarah L. Castro-Wallace Cheryl A. Nickerson |
| author_sort | Jennifer Barrila |
| collection | DOAJ |
| description | Abstract Spaceflight uniquely alters the physiology of both human cells and microbial pathogens, stimulating cellular and molecular changes directly relevant to infectious disease. However, the influence of this environment on host–pathogen interactions remains poorly understood. Here we report our results from the STL-IMMUNE study flown aboard Space Shuttle mission STS-131, which investigated multi-omic responses (transcriptomic, proteomic) of human intestinal epithelial cells to infection with Salmonella Typhimurium when both host and pathogen were simultaneously exposed to spaceflight. To our knowledge, this was the first in-flight infection and dual RNA-seq analysis using human cells. |
| first_indexed | 2024-03-11T13:46:54Z |
| format | Article |
| id | doaj.art-0a444f5908f9404283b41f24e25ddf2d |
| institution | Directory Open Access Journal |
| issn | 2373-8065 |
| language | English |
| last_indexed | 2024-03-11T13:46:54Z |
| publishDate | 2021-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Microgravity |
| spelling | doaj.art-0a444f5908f9404283b41f24e25ddf2d2023-11-02T10:06:44ZengNature Portfolionpj Microgravity2373-80652021-03-017111010.1038/s41526-021-00136-wEvaluating the effect of spaceflight on the host–pathogen interaction between human intestinal epithelial cells and Salmonella TyphimuriumJennifer Barrila0Shameema F. Sarker1Nicole Hansmeier2Shanshan Yang3Kristina Buss4Natalia Briones5Jin Park6Richard R. Davis7Rebecca J. Forsyth8C. Mark Ott9Kevin Sato10Cristine Kosnik11Anthony Yang12Cheryl Shimoda13Nicole Rayl14Diana Ly15Aaron Landenberger16Stephanie D. Wilson17Naoko Yamazaki18Jason Steel19Camila Montano20Rolf U. Halden21Tom Cannon22Sarah L. Castro-Wallace23Cheryl A. Nickerson24Biodesign Center for Fundamental and Applied Microbiomics, Arizona State UniversityBiodesign Center for Infectious Diseases and Vaccinology, Arizona State UniversitySwette Center for Environmental Biotechnology, The Biodesign Institute, Arizona State UniversityBioinformatics Core Facility, Bioscience, Knowledge Enterprise, Arizona State UniversityBioinformatics Core Facility, Bioscience, Knowledge Enterprise, Arizona State UniversityBioinformatics Core Facility, Bioscience, Knowledge Enterprise, Arizona State UniversityBioinformatics Core Facility, Bioscience, Knowledge Enterprise, Arizona State UniversityBiodesign Center for Fundamental and Applied Microbiomics, Arizona State UniversityBiodesign Center for Infectious Diseases and Vaccinology, Arizona State UniversityBiomedical Research and Environmental Sciences Division, NASA Johnson Space CenterNASA Ames Research CenterTissue Genesis, IncTissue Genesis, IncTissue Genesis, IncNASA Ames Research CenterNASA Ames Research CenterDoD Space Test ProgramAstronaut Office, NASA Johnson Space CenterSpace Biomedical Research Office, Human Space Technology and Astronauts Department, Japan Aerospace Exploration Agency (JAXA)Bioinformatics Core Facility, Bioscience, Knowledge Enterprise, Arizona State UniversityBioinformatics Core Facility, Bioscience, Knowledge Enterprise, Arizona State UniversityBiodesign Center for Environmental Health Engineering, School of Sustainable Engineering and the Built Environment, Arizona State UniversityTissue Genesis, IncBiomedical Research and Environmental Sciences Division, NASA Johnson Space CenterBiodesign Center for Fundamental and Applied Microbiomics, Arizona State UniversityAbstract Spaceflight uniquely alters the physiology of both human cells and microbial pathogens, stimulating cellular and molecular changes directly relevant to infectious disease. However, the influence of this environment on host–pathogen interactions remains poorly understood. Here we report our results from the STL-IMMUNE study flown aboard Space Shuttle mission STS-131, which investigated multi-omic responses (transcriptomic, proteomic) of human intestinal epithelial cells to infection with Salmonella Typhimurium when both host and pathogen were simultaneously exposed to spaceflight. To our knowledge, this was the first in-flight infection and dual RNA-seq analysis using human cells.https://doi.org/10.1038/s41526-021-00136-w |
| spellingShingle | Jennifer Barrila Shameema F. Sarker Nicole Hansmeier Shanshan Yang Kristina Buss Natalia Briones Jin Park Richard R. Davis Rebecca J. Forsyth C. Mark Ott Kevin Sato Cristine Kosnik Anthony Yang Cheryl Shimoda Nicole Rayl Diana Ly Aaron Landenberger Stephanie D. Wilson Naoko Yamazaki Jason Steel Camila Montano Rolf U. Halden Tom Cannon Sarah L. Castro-Wallace Cheryl A. Nickerson Evaluating the effect of spaceflight on the host–pathogen interaction between human intestinal epithelial cells and Salmonella Typhimurium npj Microgravity |
| title | Evaluating the effect of spaceflight on the host–pathogen interaction between human intestinal epithelial cells and Salmonella Typhimurium |
| title_full | Evaluating the effect of spaceflight on the host–pathogen interaction between human intestinal epithelial cells and Salmonella Typhimurium |
| title_fullStr | Evaluating the effect of spaceflight on the host–pathogen interaction between human intestinal epithelial cells and Salmonella Typhimurium |
| title_full_unstemmed | Evaluating the effect of spaceflight on the host–pathogen interaction between human intestinal epithelial cells and Salmonella Typhimurium |
| title_short | Evaluating the effect of spaceflight on the host–pathogen interaction between human intestinal epithelial cells and Salmonella Typhimurium |
| title_sort | evaluating the effect of spaceflight on the host pathogen interaction between human intestinal epithelial cells and salmonella typhimurium |
| url | https://doi.org/10.1038/s41526-021-00136-w |
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