Contribution of seedling vigour and anoxia/hypoxia-responsive genes to submergence tolerance in Vietnamese lowland rice (Oryza sativa L.)
A direct-seeded rice cultivation system has been widely adopted in Asian countries. Optimum germination and vigorous seedling growth under submergence are key traits for the practice of direct seeding. We studied the post-germination seedling vigour in Vietnamese lowland rice accessions based on thr...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2016-09-01
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| Series: | Biotechnology & Biotechnological Equipment |
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| Online Access: | http://dx.doi.org/10.1080/13102818.2016.1204944 |
| _version_ | 1818284162807758848 |
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| author | Hien Thi Thu Vu Giang Thi Nguyen Trang Thi Nguyen Tuyen Thi Minh Vo Chiharu Nakamura |
| author_facet | Hien Thi Thu Vu Giang Thi Nguyen Trang Thi Nguyen Tuyen Thi Minh Vo Chiharu Nakamura |
| author_sort | Hien Thi Thu Vu |
| collection | DOAJ |
| description | A direct-seeded rice cultivation system has been widely adopted in Asian countries. Optimum germination and vigorous seedling growth under submergence are key traits for the practice of direct seeding. We studied the post-germination seedling vigour in Vietnamese lowland rice accessions based on three bio-parameters, shoot elongation growth under five-day submergence in water-filled test-tubes, seedling recovery rate five days after transferring submerged seedlings to pots with soil and seedling survival rate 21 days after sowing seeds in nursery beds and immediate incubation under submergence. A large diversity was found in seedling vigour thus estimated among the accessions. Significantly high correlations were observed among all three bio-parameters, verifying the contribution of seedling vigour to the manifestation of submergence tolerance at this critical stage of rice development. To examine the roles of anoxia/hypoxia-responsive genes, the expression of 17 candidate genes was studied by reverse transcription polymerase chain reaction (RT-PCR) and compared between selected vigorous and non-vigorous groups of accessions. Transcripts of all but two genes showed marked accumulation in submerged seedlings. No differences, however, were found between the two contrasting groups. The observed common and coordinate expression of anoxia/hypoxia-induced genes suggests that they might assume roles in attaining baseline tolerance against submergence stress. It was also suggested that some unknown genetic factors are operating in determining cultivar/genotype-specific levels of submergence tolerance as assessed by post-germination seedling vigour. |
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| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-13T00:48:25Z |
| publishDate | 2016-09-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-0a45fd2e83214d6893febd7c4193834d2022-12-22T00:04:59ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302016-09-0130584285210.1080/13102818.2016.12049441204944Contribution of seedling vigour and anoxia/hypoxia-responsive genes to submergence tolerance in Vietnamese lowland rice (Oryza sativa L.)Hien Thi Thu Vu0Giang Thi Nguyen1Trang Thi Nguyen2Tuyen Thi Minh Vo3Chiharu Nakamura4Agricultural Genetics Institute (AGI)Agricultural Genetics Institute (AGI)Agricultural Genetics Institute (AGI)Vietnam Academy of Agricultural Sciences (VAAS), Agricultural Genetics Institute (AGI)Kobe UniversityA direct-seeded rice cultivation system has been widely adopted in Asian countries. Optimum germination and vigorous seedling growth under submergence are key traits for the practice of direct seeding. We studied the post-germination seedling vigour in Vietnamese lowland rice accessions based on three bio-parameters, shoot elongation growth under five-day submergence in water-filled test-tubes, seedling recovery rate five days after transferring submerged seedlings to pots with soil and seedling survival rate 21 days after sowing seeds in nursery beds and immediate incubation under submergence. A large diversity was found in seedling vigour thus estimated among the accessions. Significantly high correlations were observed among all three bio-parameters, verifying the contribution of seedling vigour to the manifestation of submergence tolerance at this critical stage of rice development. To examine the roles of anoxia/hypoxia-responsive genes, the expression of 17 candidate genes was studied by reverse transcription polymerase chain reaction (RT-PCR) and compared between selected vigorous and non-vigorous groups of accessions. Transcripts of all but two genes showed marked accumulation in submerged seedlings. No differences, however, were found between the two contrasting groups. The observed common and coordinate expression of anoxia/hypoxia-induced genes suggests that they might assume roles in attaining baseline tolerance against submergence stress. It was also suggested that some unknown genetic factors are operating in determining cultivar/genotype-specific levels of submergence tolerance as assessed by post-germination seedling vigour.http://dx.doi.org/10.1080/13102818.2016.1204944Anoxia/hypoxiaphenotypic assaygene expressionrice (Oryza sativa L.)submergence tolerance |
| spellingShingle | Hien Thi Thu Vu Giang Thi Nguyen Trang Thi Nguyen Tuyen Thi Minh Vo Chiharu Nakamura Contribution of seedling vigour and anoxia/hypoxia-responsive genes to submergence tolerance in Vietnamese lowland rice (Oryza sativa L.) Biotechnology & Biotechnological Equipment Anoxia/hypoxia phenotypic assay gene expression rice (Oryza sativa L.) submergence tolerance |
| title | Contribution of seedling vigour and anoxia/hypoxia-responsive genes to submergence tolerance in Vietnamese lowland rice (Oryza sativa L.) |
| title_full | Contribution of seedling vigour and anoxia/hypoxia-responsive genes to submergence tolerance in Vietnamese lowland rice (Oryza sativa L.) |
| title_fullStr | Contribution of seedling vigour and anoxia/hypoxia-responsive genes to submergence tolerance in Vietnamese lowland rice (Oryza sativa L.) |
| title_full_unstemmed | Contribution of seedling vigour and anoxia/hypoxia-responsive genes to submergence tolerance in Vietnamese lowland rice (Oryza sativa L.) |
| title_short | Contribution of seedling vigour and anoxia/hypoxia-responsive genes to submergence tolerance in Vietnamese lowland rice (Oryza sativa L.) |
| title_sort | contribution of seedling vigour and anoxia hypoxia responsive genes to submergence tolerance in vietnamese lowland rice oryza sativa l |
| topic | Anoxia/hypoxia phenotypic assay gene expression rice (Oryza sativa L.) submergence tolerance |
| url | http://dx.doi.org/10.1080/13102818.2016.1204944 |
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