Feline Stool-Associated Circular DNA Virus (FeSCV) in Diarrheic Cats in China
Feline stool-associated circular DNA virus (FeSCV) is an unclassified circular replication-associated protein-encoding single-stranded (CRESS) DNA virus that was discovered in cats in Japan in 2018. Few studies on the genomic characteristics and prevalence of FeSCV have been conducted. To investigat...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-06-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2021.694089/full |
| _version_ | 1818613481490874368 |
|---|---|
| author | Xiangqi Hao Xiangqi Hao Xiangqi Hao Yanchao Li Yanchao Li Yanchao Li Xinkai Hu Xinkai Hu Xinkai Hu Xueying Fu Xueying Fu Xueying Fu Jie Dong Jie Dong Jie Dong Haoyang Zhang Haoyang Zhang Haoyang Zhang Pei Zhou Pei Zhou Pei Zhou Shoujun Li Shoujun Li Shoujun Li |
| author_facet | Xiangqi Hao Xiangqi Hao Xiangqi Hao Yanchao Li Yanchao Li Yanchao Li Xinkai Hu Xinkai Hu Xinkai Hu Xueying Fu Xueying Fu Xueying Fu Jie Dong Jie Dong Jie Dong Haoyang Zhang Haoyang Zhang Haoyang Zhang Pei Zhou Pei Zhou Pei Zhou Shoujun Li Shoujun Li Shoujun Li |
| author_sort | Xiangqi Hao |
| collection | DOAJ |
| description | Feline stool-associated circular DNA virus (FeSCV) is an unclassified circular replication-associated protein-encoding single-stranded (CRESS) DNA virus that was discovered in cats in Japan in 2018. Few studies on the genomic characteristics and prevalence of FeSCV have been conducted. To investigate whether FeSCV has been circulating in domestic cats in Guangdong, China, fecal samples were collected from cats with diarrhea in an animal hospital in 2018 to promote research on FeSCV. The FeSCV genome was obtained by PCR amplification and sequencing, and the detected virus was named PY4 (GenBank No. MT732515). The genome of PY4 was 2,034 nt in size, which was 12 nt smaller than the reported genome of Japanese FeSCV strains (KU7, KU8, KU9, KU14) (2,046 nt). The PY4 strain shared 95.1 ~ 95.5% homology with Japanese FeSCV strains. Notably, the Cap protein of PY4 was mutated at 15 amino acid sites, and the PY4 genome contained a unique open reading frame 3. In addition, there were two additional base insertions in the stem-loop structure of PY4, and the nucleotide homology of the spacer region was not high. A phylogenetic tree based on Rep proteins showed that PY4, Japanese FeSCVs and rodent stool-associated circular viruses (RodSCVs) clustered together, suggesting that they might share a similar origin in their phylogenetic evolution. In this study, samples collected in Guangzhou, China, in 2018 were subjected to an etiological investigation, and 20% (2/10) of the samples were positive for FeSCV. The ORFs, stem-loop structures, Cap proteins and intergenic region sequences of PY4 were significantly different from those reported in Japan. This is the first report of FeSCV in domestic cats with diarrhea in China, and further epidemiological studies are urgently needed to assess the impact of the virus on cats. |
| first_indexed | 2024-12-16T16:02:48Z |
| format | Article |
| id | doaj.art-0a4a7d85968940dc92f656d7dca74c34 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-16T16:02:48Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-0a4a7d85968940dc92f656d7dca74c342022-12-21T22:25:26ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-06-01810.3389/fvets.2021.694089694089Feline Stool-Associated Circular DNA Virus (FeSCV) in Diarrheic Cats in ChinaXiangqi Hao0Xiangqi Hao1Xiangqi Hao2Yanchao Li3Yanchao Li4Yanchao Li5Xinkai Hu6Xinkai Hu7Xinkai Hu8Xueying Fu9Xueying Fu10Xueying Fu11Jie Dong12Jie Dong13Jie Dong14Haoyang Zhang15Haoyang Zhang16Haoyang Zhang17Pei Zhou18Pei Zhou19Pei Zhou20Shoujun Li21Shoujun Li22Shoujun Li23College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, ChinaGuangdong Provincial Pet Engineering Technology Research Center, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, ChinaGuangdong Provincial Pet Engineering Technology Research Center, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, ChinaGuangdong Provincial Pet Engineering Technology Research Center, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, ChinaGuangdong Provincial Pet Engineering Technology Research Center, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, ChinaGuangdong Provincial Pet Engineering Technology Research Center, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, ChinaGuangdong Provincial Pet Engineering Technology Research Center, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, ChinaGuangdong Provincial Pet Engineering Technology Research Center, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, ChinaGuangdong Provincial Pet Engineering Technology Research Center, Guangzhou, ChinaFeline stool-associated circular DNA virus (FeSCV) is an unclassified circular replication-associated protein-encoding single-stranded (CRESS) DNA virus that was discovered in cats in Japan in 2018. Few studies on the genomic characteristics and prevalence of FeSCV have been conducted. To investigate whether FeSCV has been circulating in domestic cats in Guangdong, China, fecal samples were collected from cats with diarrhea in an animal hospital in 2018 to promote research on FeSCV. The FeSCV genome was obtained by PCR amplification and sequencing, and the detected virus was named PY4 (GenBank No. MT732515). The genome of PY4 was 2,034 nt in size, which was 12 nt smaller than the reported genome of Japanese FeSCV strains (KU7, KU8, KU9, KU14) (2,046 nt). The PY4 strain shared 95.1 ~ 95.5% homology with Japanese FeSCV strains. Notably, the Cap protein of PY4 was mutated at 15 amino acid sites, and the PY4 genome contained a unique open reading frame 3. In addition, there were two additional base insertions in the stem-loop structure of PY4, and the nucleotide homology of the spacer region was not high. A phylogenetic tree based on Rep proteins showed that PY4, Japanese FeSCVs and rodent stool-associated circular viruses (RodSCVs) clustered together, suggesting that they might share a similar origin in their phylogenetic evolution. In this study, samples collected in Guangzhou, China, in 2018 were subjected to an etiological investigation, and 20% (2/10) of the samples were positive for FeSCV. The ORFs, stem-loop structures, Cap proteins and intergenic region sequences of PY4 were significantly different from those reported in Japan. This is the first report of FeSCV in domestic cats with diarrhea in China, and further epidemiological studies are urgently needed to assess the impact of the virus on cats.https://www.frontiersin.org/articles/10.3389/fvets.2021.694089/fullfeline stool-associated circular DNA virus (FeSCV)catsChinagenetic characterizationdiarrheic |
| spellingShingle | Xiangqi Hao Xiangqi Hao Xiangqi Hao Yanchao Li Yanchao Li Yanchao Li Xinkai Hu Xinkai Hu Xinkai Hu Xueying Fu Xueying Fu Xueying Fu Jie Dong Jie Dong Jie Dong Haoyang Zhang Haoyang Zhang Haoyang Zhang Pei Zhou Pei Zhou Pei Zhou Shoujun Li Shoujun Li Shoujun Li Feline Stool-Associated Circular DNA Virus (FeSCV) in Diarrheic Cats in China Frontiers in Veterinary Science feline stool-associated circular DNA virus (FeSCV) cats China genetic characterization diarrheic |
| title | Feline Stool-Associated Circular DNA Virus (FeSCV) in Diarrheic Cats in China |
| title_full | Feline Stool-Associated Circular DNA Virus (FeSCV) in Diarrheic Cats in China |
| title_fullStr | Feline Stool-Associated Circular DNA Virus (FeSCV) in Diarrheic Cats in China |
| title_full_unstemmed | Feline Stool-Associated Circular DNA Virus (FeSCV) in Diarrheic Cats in China |
| title_short | Feline Stool-Associated Circular DNA Virus (FeSCV) in Diarrheic Cats in China |
| title_sort | feline stool associated circular dna virus fescv in diarrheic cats in china |
| topic | feline stool-associated circular DNA virus (FeSCV) cats China genetic characterization diarrheic |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2021.694089/full |
| work_keys_str_mv | AT xiangqihao felinestoolassociatedcirculardnavirusfescvindiarrheiccatsinchina AT xiangqihao felinestoolassociatedcirculardnavirusfescvindiarrheiccatsinchina AT xiangqihao felinestoolassociatedcirculardnavirusfescvindiarrheiccatsinchina AT yanchaoli felinestoolassociatedcirculardnavirusfescvindiarrheiccatsinchina AT yanchaoli felinestoolassociatedcirculardnavirusfescvindiarrheiccatsinchina AT yanchaoli felinestoolassociatedcirculardnavirusfescvindiarrheiccatsinchina AT xinkaihu felinestoolassociatedcirculardnavirusfescvindiarrheiccatsinchina AT xinkaihu felinestoolassociatedcirculardnavirusfescvindiarrheiccatsinchina AT xinkaihu felinestoolassociatedcirculardnavirusfescvindiarrheiccatsinchina AT xueyingfu felinestoolassociatedcirculardnavirusfescvindiarrheiccatsinchina AT xueyingfu felinestoolassociatedcirculardnavirusfescvindiarrheiccatsinchina AT xueyingfu felinestoolassociatedcirculardnavirusfescvindiarrheiccatsinchina AT jiedong felinestoolassociatedcirculardnavirusfescvindiarrheiccatsinchina AT jiedong felinestoolassociatedcirculardnavirusfescvindiarrheiccatsinchina AT jiedong felinestoolassociatedcirculardnavirusfescvindiarrheiccatsinchina AT haoyangzhang felinestoolassociatedcirculardnavirusfescvindiarrheiccatsinchina AT haoyangzhang felinestoolassociatedcirculardnavirusfescvindiarrheiccatsinchina AT haoyangzhang felinestoolassociatedcirculardnavirusfescvindiarrheiccatsinchina AT peizhou felinestoolassociatedcirculardnavirusfescvindiarrheiccatsinchina AT peizhou felinestoolassociatedcirculardnavirusfescvindiarrheiccatsinchina AT peizhou felinestoolassociatedcirculardnavirusfescvindiarrheiccatsinchina AT shoujunli felinestoolassociatedcirculardnavirusfescvindiarrheiccatsinchina AT shoujunli felinestoolassociatedcirculardnavirusfescvindiarrheiccatsinchina AT shoujunli felinestoolassociatedcirculardnavirusfescvindiarrheiccatsinchina |