Isolation of skin-derived precursors from human foreskin and their differentiation into neurons and glial cells
"n Normal 0 false false false EN-US X-NONE AR-SA MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:y...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | fas |
| Published: |
Tehran University of Medical Sciences
2010-12-01
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| Series: | Tehran University Medical Journal |
| Subjects: | |
| Online Access: | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/17239.pdf&manuscript_id=17239 |
| _version_ | 1818594743963090944 |
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| author | Bakhtiari M Mansouri K Mostafaie A Sadeghi Y Mozafari H Ghorbani R Rezaei Tavirani M |
| author_facet | Bakhtiari M Mansouri K Mostafaie A Sadeghi Y Mozafari H Ghorbani R Rezaei Tavirani M |
| author_sort | Bakhtiari M |
| collection | DOAJ |
| description | "n Normal 0 false false false EN-US X-NONE AR-SA MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:Arial; mso-bidi-theme-font:minor-bidi;} Background: Skin-derived precursors (SKPs) are a type of progenitor cells extracted from mammalian dermal tissue and can be differentiate to neural and mesodermal lineage in vitro. These cells can introduce an accessible autologos source of neural precursor cells for treatment of different neurodegenerative diseases. This research was done in order to set up isolation, culture, proliferation and differentiation of human skin derived precursors (hSKPs)."n"nMethods: Human foreskin samples were cut into smaller pieces and cultured in proliferation medium after enzymatic digestion. To induce neural differentiation, cells were cultured in neural differentiation medium after fifth passage. We used immunocytochemistry and RT-PCR for characterization of the cells. Neuron and glial cell differentiation potential was assessed by immunofloresence using specific antibodies. The experiments were carried out in triplicate."n"nResults: After differentiation, βΙΙΙ- tubulin and neurofilament-M positive cells were observed that are specific markers for neurons. Moreover, glial fibrillary acid protein (GFAP) and S100 positive cells were identified that are markers specifically express in glial cells. Detected neurons and glials were also confirmed by their morphologic characterizations."n"nConclusion: Our results demonstrated that skin-derived precursors obtained from human foreskin can exhibit neuronal and glial differentiation potential in vitro, depending on the protocols of induction. |
| first_indexed | 2024-12-16T11:04:59Z |
| format | Article |
| id | doaj.art-0a4cc688bef843b18ad41a6fe368c670 |
| institution | Directory Open Access Journal |
| issn | 1683-1764 1735-7322 |
| language | fas |
| last_indexed | 2024-12-16T11:04:59Z |
| publishDate | 2010-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Tehran University Medical Journal |
| spelling | doaj.art-0a4cc688bef843b18ad41a6fe368c6702022-12-21T22:33:52ZfasTehran University of Medical SciencesTehran University Medical Journal1683-17641735-73222010-12-01689508515Isolation of skin-derived precursors from human foreskin and their differentiation into neurons and glial cellsBakhtiari MMansouri KMostafaie ASadeghi YMozafari HGhorbani RRezaei Tavirani M"n Normal 0 false false false EN-US X-NONE AR-SA MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:Arial; mso-bidi-theme-font:minor-bidi;} Background: Skin-derived precursors (SKPs) are a type of progenitor cells extracted from mammalian dermal tissue and can be differentiate to neural and mesodermal lineage in vitro. These cells can introduce an accessible autologos source of neural precursor cells for treatment of different neurodegenerative diseases. This research was done in order to set up isolation, culture, proliferation and differentiation of human skin derived precursors (hSKPs)."n"nMethods: Human foreskin samples were cut into smaller pieces and cultured in proliferation medium after enzymatic digestion. To induce neural differentiation, cells were cultured in neural differentiation medium after fifth passage. We used immunocytochemistry and RT-PCR for characterization of the cells. Neuron and glial cell differentiation potential was assessed by immunofloresence using specific antibodies. The experiments were carried out in triplicate."n"nResults: After differentiation, βΙΙΙ- tubulin and neurofilament-M positive cells were observed that are specific markers for neurons. Moreover, glial fibrillary acid protein (GFAP) and S100 positive cells were identified that are markers specifically express in glial cells. Detected neurons and glials were also confirmed by their morphologic characterizations."n"nConclusion: Our results demonstrated that skin-derived precursors obtained from human foreskin can exhibit neuronal and glial differentiation potential in vitro, depending on the protocols of induction.http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/17239.pdf&manuscript_id=17239Skin-derived precursorneural lineageneuronglial celldifferentiation |
| spellingShingle | Bakhtiari M Mansouri K Mostafaie A Sadeghi Y Mozafari H Ghorbani R Rezaei Tavirani M Isolation of skin-derived precursors from human foreskin and their differentiation into neurons and glial cells Tehran University Medical Journal Skin-derived precursor neural lineage neuron glial cell differentiation |
| title | Isolation of skin-derived precursors from human foreskin and their differentiation into neurons and glial cells |
| title_full | Isolation of skin-derived precursors from human foreskin and their differentiation into neurons and glial cells |
| title_fullStr | Isolation of skin-derived precursors from human foreskin and their differentiation into neurons and glial cells |
| title_full_unstemmed | Isolation of skin-derived precursors from human foreskin and their differentiation into neurons and glial cells |
| title_short | Isolation of skin-derived precursors from human foreskin and their differentiation into neurons and glial cells |
| title_sort | isolation of skin derived precursors from human foreskin and their differentiation into neurons and glial cells |
| topic | Skin-derived precursor neural lineage neuron glial cell differentiation |
| url | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/17239.pdf&manuscript_id=17239 |
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