ROLE OF VITAMIN E (α TOCOPHEROL) TO PREVENT THE SPERMATOGONIA, SERTOLI CELL, AND LEYDIG CELL DAMAGE IN RATS TESTICLE (STRAIN WISTAR) AFTER CISPLATIN TREATMENT
Objective: To determine the difference in spermatogonium cells, leydig cells and sertoli cells count in white rats testicle (wistar strains) obtained with the combination of cisplatin and vitamin E compared with that only received cisplatin. Material & Methods: There were 4 random groups out of...
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| Format: | Article |
| Language: | English |
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IKATAN AHLI UROLOGI INDONESIA
2019-07-01
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| Series: | Jurnal Urologi Indonesia |
| Subjects: | |
| Online Access: | http://www.urologi.or.id/juri/juri/article/view/519 |
| _version_ | 1818791984167387136 |
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| author | Rahmad Krismantoro Fikri Rizaldi Doddy M Soebadi |
| author_facet | Rahmad Krismantoro Fikri Rizaldi Doddy M Soebadi |
| author_sort | Rahmad Krismantoro |
| collection | DOAJ |
| description | Objective: To determine the difference in spermatogonium cells, leydig cells and sertoli cells count in white rats testicle (wistar strains) obtained with the combination of cisplatin and vitamin E compared with that only received cisplatin. Material & Methods: There were 4 random groups out of a total of twenty four winstar strain rats (n=6). The control group (I) injected normal saline 0.9% intraperitoneally (i.p.) as the placebo on the 3rd week. Group (II) given cisplatin (5 mg/kgbw) injection i.p. on 3rd week, Group (III) given cisplatin injection 5 mg/kgbw i.p. on 3rd week + vitamin E 50 mg/kgbw by gavage for 7 weeks and group (IV) cisplatin injection 5mg/kgbw i.p. on 3rd week + vitamin E 200 mg/kgbw by gavage for 7 weeks. Vitamin E was given 3 weeks before up to 4 weeks after cisplatin injection (total 7 weeks). Observations by calculating the average number of spermatogonia, sertoli and leydig cells on a cross-sectional section of the seminiferous tubule with Haematoxylin-Eosin staining using a 400x light magnification microscope. Results: Cisplatin decreases spermatogonia, sertoli, and leydig cells significantly against control. Vitamin E 200 mg/kgbw significantly increased the number of spermatogonium, sertoli, and leydig cells (p<0.05) compared to group in combination with vitamin E 50 mg/kg bw and cisplatin or cisplatin only group. Only leydig cells count was significantly increased in the combination group of vitamin E 50 mg/kgbw and cisplatin compared to the cisplatin group. Conclusion: Vitamin E 200 mg/kgbw provides a protective effect against decreased spermatogonia, sertoli and leydig cells due to cisplatin 5 mg/kgbw exposure which its protectivity depends on the given dose. |
| first_indexed | 2024-12-18T15:20:02Z |
| format | Article |
| id | doaj.art-0a4ece8e3a51414ea917399501fa4bab |
| institution | Directory Open Access Journal |
| issn | 0853-442X 2355-1402 |
| language | English |
| last_indexed | 2024-12-18T15:20:02Z |
| publishDate | 2019-07-01 |
| publisher | IKATAN AHLI UROLOGI INDONESIA |
| record_format | Article |
| series | Jurnal Urologi Indonesia |
| spelling | doaj.art-0a4ece8e3a51414ea917399501fa4bab2022-12-21T21:03:25ZengIKATAN AHLI UROLOGI INDONESIAJurnal Urologi Indonesia0853-442X2355-14022019-07-0126210.32421/juri.v26i2.519ROLE OF VITAMIN E (α TOCOPHEROL) TO PREVENT THE SPERMATOGONIA, SERTOLI CELL, AND LEYDIG CELL DAMAGE IN RATS TESTICLE (STRAIN WISTAR) AFTER CISPLATIN TREATMENTRahmad Krismantoro0Fikri Rizaldi1Doddy M Soebadi2Department of Urology, Faculty of Medicine/Universitas Airlangga, Soetomo General Hospital, SurabayaDepartment of Urology, Faculty of Medicine/Universitas Airlangga, Soetomo General Hospital, SurabayaDepartment of Urology, Faculty of Medicine/Universitas Airlangga, Soetomo General Hospital, SurabayaObjective: To determine the difference in spermatogonium cells, leydig cells and sertoli cells count in white rats testicle (wistar strains) obtained with the combination of cisplatin and vitamin E compared with that only received cisplatin. Material & Methods: There were 4 random groups out of a total of twenty four winstar strain rats (n=6). The control group (I) injected normal saline 0.9% intraperitoneally (i.p.) as the placebo on the 3rd week. Group (II) given cisplatin (5 mg/kgbw) injection i.p. on 3rd week, Group (III) given cisplatin injection 5 mg/kgbw i.p. on 3rd week + vitamin E 50 mg/kgbw by gavage for 7 weeks and group (IV) cisplatin injection 5mg/kgbw i.p. on 3rd week + vitamin E 200 mg/kgbw by gavage for 7 weeks. Vitamin E was given 3 weeks before up to 4 weeks after cisplatin injection (total 7 weeks). Observations by calculating the average number of spermatogonia, sertoli and leydig cells on a cross-sectional section of the seminiferous tubule with Haematoxylin-Eosin staining using a 400x light magnification microscope. Results: Cisplatin decreases spermatogonia, sertoli, and leydig cells significantly against control. Vitamin E 200 mg/kgbw significantly increased the number of spermatogonium, sertoli, and leydig cells (p<0.05) compared to group in combination with vitamin E 50 mg/kg bw and cisplatin or cisplatin only group. Only leydig cells count was significantly increased in the combination group of vitamin E 50 mg/kgbw and cisplatin compared to the cisplatin group. Conclusion: Vitamin E 200 mg/kgbw provides a protective effect against decreased spermatogonia, sertoli and leydig cells due to cisplatin 5 mg/kgbw exposure which its protectivity depends on the given dose.http://www.urologi.or.id/juri/juri/article/view/519vitamin espermatogonium cellssertoli cellsleydig cellscisplatin |
| spellingShingle | Rahmad Krismantoro Fikri Rizaldi Doddy M Soebadi ROLE OF VITAMIN E (α TOCOPHEROL) TO PREVENT THE SPERMATOGONIA, SERTOLI CELL, AND LEYDIG CELL DAMAGE IN RATS TESTICLE (STRAIN WISTAR) AFTER CISPLATIN TREATMENT Jurnal Urologi Indonesia vitamin e spermatogonium cells sertoli cells leydig cells cisplatin |
| title | ROLE OF VITAMIN E (α TOCOPHEROL) TO PREVENT THE SPERMATOGONIA, SERTOLI CELL, AND LEYDIG CELL DAMAGE IN RATS TESTICLE (STRAIN WISTAR) AFTER CISPLATIN TREATMENT |
| title_full | ROLE OF VITAMIN E (α TOCOPHEROL) TO PREVENT THE SPERMATOGONIA, SERTOLI CELL, AND LEYDIG CELL DAMAGE IN RATS TESTICLE (STRAIN WISTAR) AFTER CISPLATIN TREATMENT |
| title_fullStr | ROLE OF VITAMIN E (α TOCOPHEROL) TO PREVENT THE SPERMATOGONIA, SERTOLI CELL, AND LEYDIG CELL DAMAGE IN RATS TESTICLE (STRAIN WISTAR) AFTER CISPLATIN TREATMENT |
| title_full_unstemmed | ROLE OF VITAMIN E (α TOCOPHEROL) TO PREVENT THE SPERMATOGONIA, SERTOLI CELL, AND LEYDIG CELL DAMAGE IN RATS TESTICLE (STRAIN WISTAR) AFTER CISPLATIN TREATMENT |
| title_short | ROLE OF VITAMIN E (α TOCOPHEROL) TO PREVENT THE SPERMATOGONIA, SERTOLI CELL, AND LEYDIG CELL DAMAGE IN RATS TESTICLE (STRAIN WISTAR) AFTER CISPLATIN TREATMENT |
| title_sort | role of vitamin e α tocopherol to prevent the spermatogonia sertoli cell and leydig cell damage in rats testicle strain wistar after cisplatin treatment |
| topic | vitamin e spermatogonium cells sertoli cells leydig cells cisplatin |
| url | http://www.urologi.or.id/juri/juri/article/view/519 |
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