Decline of influenza-specific CD8<sup>+ </sup>T cell repertoire in healthy geriatric donors
<p>Abstract</p> <p>Background</p> <p>While influenza vaccination results in protective antibodies against primary infections, clearance of infection is primarily mediated through CD8<sup>+ </sup>T cells. Studying the CD8<sup>+ </sup>T cell respon...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-08-01
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| Series: | Immunity & Ageing |
| Online Access: | http://www.immunityageing.com/content/8/1/6 |
| _version_ | 1818060409370836992 |
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| author | Ramachandra Lakshmi Oelke Mathias Lee Jessica B Canaday David H Schneck Jonathan P |
| author_facet | Ramachandra Lakshmi Oelke Mathias Lee Jessica B Canaday David H Schneck Jonathan P |
| author_sort | Ramachandra Lakshmi |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>While influenza vaccination results in protective antibodies against primary infections, clearance of infection is primarily mediated through CD8<sup>+ </sup>T cells. Studying the CD8<sup>+ </sup>T cell response to influenza epitopes is crucial in understanding the disease associated morbidity and mortality especially in at risk populations such as the elderly. We compared the CD8<sup>+ </sup>T cell response to immunodominant and subdominant influenza epitopes in HLA-A2<sup>+ </sup>control, adult donors, aged 21-42, and in geriatric donors, aged 65 and older.</p> <p>Results</p> <p>We used a novel artificial Antigen Presenting Cell (aAPC) based stimulation assay to reveal responses that could not be detected by enzyme-linked immunosorbent spot (ELISpot). 14 younger control donors and 12 geriatric donors were enrolled in this study. The mean number of influenza-specific subdominant epitopes per control donor detected by ELISpot was only 1.4 while the mean detected by aAPC assay was 3.3 (p = 0.0096). Using the aAPC assay, 92% of the control donors responded to at least one subdominant epitopes, while 71% of control donors responded to more than one subdominant influenza-specific response. 66% of geriatric donors lacked a subdominant influenza-specific response and 33% of geriatric donors responded to only 1 subdominant epitope. The difference in subdominant response between age groups is statistically significant (p = 0.0003).</p> <p>Conclusion</p> <p>Geriatric donors lacked the broad, multi-specific response to subdominant epitopes seen in the control donors. Thus, we conclude that aging leads to a decrease in the subdominant influenza-specific CTL responses which may contribute to the increased morbidity and mortality in older individuals.</p> |
| first_indexed | 2024-12-10T13:31:58Z |
| format | Article |
| id | doaj.art-0a509a0e8ef849a4ad8a7b862cf60ba9 |
| institution | Directory Open Access Journal |
| issn | 1742-4933 |
| language | English |
| last_indexed | 2024-12-10T13:31:58Z |
| publishDate | 2011-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Immunity & Ageing |
| spelling | doaj.art-0a509a0e8ef849a4ad8a7b862cf60ba92022-12-22T01:46:55ZengBMCImmunity & Ageing1742-49332011-08-0181610.1186/1742-4933-8-6Decline of influenza-specific CD8<sup>+ </sup>T cell repertoire in healthy geriatric donorsRamachandra LakshmiOelke MathiasLee Jessica BCanaday David HSchneck Jonathan P<p>Abstract</p> <p>Background</p> <p>While influenza vaccination results in protective antibodies against primary infections, clearance of infection is primarily mediated through CD8<sup>+ </sup>T cells. Studying the CD8<sup>+ </sup>T cell response to influenza epitopes is crucial in understanding the disease associated morbidity and mortality especially in at risk populations such as the elderly. We compared the CD8<sup>+ </sup>T cell response to immunodominant and subdominant influenza epitopes in HLA-A2<sup>+ </sup>control, adult donors, aged 21-42, and in geriatric donors, aged 65 and older.</p> <p>Results</p> <p>We used a novel artificial Antigen Presenting Cell (aAPC) based stimulation assay to reveal responses that could not be detected by enzyme-linked immunosorbent spot (ELISpot). 14 younger control donors and 12 geriatric donors were enrolled in this study. The mean number of influenza-specific subdominant epitopes per control donor detected by ELISpot was only 1.4 while the mean detected by aAPC assay was 3.3 (p = 0.0096). Using the aAPC assay, 92% of the control donors responded to at least one subdominant epitopes, while 71% of control donors responded to more than one subdominant influenza-specific response. 66% of geriatric donors lacked a subdominant influenza-specific response and 33% of geriatric donors responded to only 1 subdominant epitope. The difference in subdominant response between age groups is statistically significant (p = 0.0003).</p> <p>Conclusion</p> <p>Geriatric donors lacked the broad, multi-specific response to subdominant epitopes seen in the control donors. Thus, we conclude that aging leads to a decrease in the subdominant influenza-specific CTL responses which may contribute to the increased morbidity and mortality in older individuals.</p>http://www.immunityageing.com/content/8/1/6 |
| spellingShingle | Ramachandra Lakshmi Oelke Mathias Lee Jessica B Canaday David H Schneck Jonathan P Decline of influenza-specific CD8<sup>+ </sup>T cell repertoire in healthy geriatric donors Immunity & Ageing |
| title | Decline of influenza-specific CD8<sup>+ </sup>T cell repertoire in healthy geriatric donors |
| title_full | Decline of influenza-specific CD8<sup>+ </sup>T cell repertoire in healthy geriatric donors |
| title_fullStr | Decline of influenza-specific CD8<sup>+ </sup>T cell repertoire in healthy geriatric donors |
| title_full_unstemmed | Decline of influenza-specific CD8<sup>+ </sup>T cell repertoire in healthy geriatric donors |
| title_short | Decline of influenza-specific CD8<sup>+ </sup>T cell repertoire in healthy geriatric donors |
| title_sort | decline of influenza specific cd8 sup sup t cell repertoire in healthy geriatric donors |
| url | http://www.immunityageing.com/content/8/1/6 |
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