Chemical and Physical Culture Conditions Significantly Influence the Cell Mass and Docosahexaenoic Acid Content of <i>Aurantiochytrium limacinum</i> Strain PKU#SW8
Thraustochytrids are well-known unicellular heterotrophic marine protists because of their promising ability to accumulate docosahexaenoic acid (DHA). However, the implications of their unique genomic and metabolic features on DHA production remain poorly understood. Here, the effects of chemical an...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/19/12/671 |
| _version_ | 1797502758062915584 |
|---|---|
| author | Xiaohong Chen Biswarup Sen Sai Zhang Mohan Bai Yaodong He Guangyi Wang |
| author_facet | Xiaohong Chen Biswarup Sen Sai Zhang Mohan Bai Yaodong He Guangyi Wang |
| author_sort | Xiaohong Chen |
| collection | DOAJ |
| description | Thraustochytrids are well-known unicellular heterotrophic marine protists because of their promising ability to accumulate docosahexaenoic acid (DHA). However, the implications of their unique genomic and metabolic features on DHA production remain poorly understood. Here, the effects of chemical and physical culture conditions on the cell mass and DHA production were investigated for a unique thraustochytrid strain, PKU#SW8, isolated from the seawater of Pearl River Estuary. All the tested fermentation parameters showed a significant influence on the cell mass and concentration and yield of DHA. The addition of monosaccharides (fructose, mannose, glucose, or galactose) or glycerol to the culture medium yielded much higher cell mass and DHA concentrations than that of disaccharides and starch. Similarly, organic nitrogen sources (peptone, yeast extract, tryptone, and sodium glutamate) proved to be beneficial in achieving a higher cell mass and DHA concentration. PKU#SW8 was found to grow and accumulate a considerable amount of DHA over wide ranges of KH<sub>2</sub>PO<sub>4</sub> (0.125–1.0 g/L), salinity (0–140% seawater), pH (3–9), temperature (16–36 °C), and agitation (140–230 rpm). With the optimal culture conditions (glycerol, 20 g/L; peptone, 2.5 g/L; 80% seawater; pH 4.0; 28 °C; and 200 rpm) determined based on the shake-flask experiments, the cell mass and concentration and yield of DHA were improved up to 7.5 ± 0.05 g/L, 2.14 ± 0.03 g/L, and 282.9 ± 3.0 mg/g, respectively, on a 5-L scale fermentation. This study provides valuable information about the fermentation conditions of the PKU#SW8 strain and its unique physiological features, which could be beneficial for strain development and large-scale DHA production. |
| first_indexed | 2024-03-10T03:40:41Z |
| format | Article |
| id | doaj.art-0a51977fd78544678ce451543b0c939c |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T03:40:41Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-0a51977fd78544678ce451543b0c939c2023-11-23T09:18:01ZengMDPI AGMarine Drugs1660-33972021-11-01191267110.3390/md19120671Chemical and Physical Culture Conditions Significantly Influence the Cell Mass and Docosahexaenoic Acid Content of <i>Aurantiochytrium limacinum</i> Strain PKU#SW8Xiaohong Chen0Biswarup Sen1Sai Zhang2Mohan Bai3Yaodong He4Guangyi Wang5Center of Marine Environmental Ecology, School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, ChinaCenter of Marine Environmental Ecology, School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, ChinaPolar Research Institute of China, Shanghai 200136, ChinaCollege of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaCenter of Marine Environmental Ecology, School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, ChinaCenter of Marine Environmental Ecology, School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, ChinaThraustochytrids are well-known unicellular heterotrophic marine protists because of their promising ability to accumulate docosahexaenoic acid (DHA). However, the implications of their unique genomic and metabolic features on DHA production remain poorly understood. Here, the effects of chemical and physical culture conditions on the cell mass and DHA production were investigated for a unique thraustochytrid strain, PKU#SW8, isolated from the seawater of Pearl River Estuary. All the tested fermentation parameters showed a significant influence on the cell mass and concentration and yield of DHA. The addition of monosaccharides (fructose, mannose, glucose, or galactose) or glycerol to the culture medium yielded much higher cell mass and DHA concentrations than that of disaccharides and starch. Similarly, organic nitrogen sources (peptone, yeast extract, tryptone, and sodium glutamate) proved to be beneficial in achieving a higher cell mass and DHA concentration. PKU#SW8 was found to grow and accumulate a considerable amount of DHA over wide ranges of KH<sub>2</sub>PO<sub>4</sub> (0.125–1.0 g/L), salinity (0–140% seawater), pH (3–9), temperature (16–36 °C), and agitation (140–230 rpm). With the optimal culture conditions (glycerol, 20 g/L; peptone, 2.5 g/L; 80% seawater; pH 4.0; 28 °C; and 200 rpm) determined based on the shake-flask experiments, the cell mass and concentration and yield of DHA were improved up to 7.5 ± 0.05 g/L, 2.14 ± 0.03 g/L, and 282.9 ± 3.0 mg/g, respectively, on a 5-L scale fermentation. This study provides valuable information about the fermentation conditions of the PKU#SW8 strain and its unique physiological features, which could be beneficial for strain development and large-scale DHA production.https://www.mdpi.com/1660-3397/19/12/671thraustochytridsfatty acidsdocosahexaenoic acidcell massmonosaccharidessalinity |
| spellingShingle | Xiaohong Chen Biswarup Sen Sai Zhang Mohan Bai Yaodong He Guangyi Wang Chemical and Physical Culture Conditions Significantly Influence the Cell Mass and Docosahexaenoic Acid Content of <i>Aurantiochytrium limacinum</i> Strain PKU#SW8 Marine Drugs thraustochytrids fatty acids docosahexaenoic acid cell mass monosaccharides salinity |
| title | Chemical and Physical Culture Conditions Significantly Influence the Cell Mass and Docosahexaenoic Acid Content of <i>Aurantiochytrium limacinum</i> Strain PKU#SW8 |
| title_full | Chemical and Physical Culture Conditions Significantly Influence the Cell Mass and Docosahexaenoic Acid Content of <i>Aurantiochytrium limacinum</i> Strain PKU#SW8 |
| title_fullStr | Chemical and Physical Culture Conditions Significantly Influence the Cell Mass and Docosahexaenoic Acid Content of <i>Aurantiochytrium limacinum</i> Strain PKU#SW8 |
| title_full_unstemmed | Chemical and Physical Culture Conditions Significantly Influence the Cell Mass and Docosahexaenoic Acid Content of <i>Aurantiochytrium limacinum</i> Strain PKU#SW8 |
| title_short | Chemical and Physical Culture Conditions Significantly Influence the Cell Mass and Docosahexaenoic Acid Content of <i>Aurantiochytrium limacinum</i> Strain PKU#SW8 |
| title_sort | chemical and physical culture conditions significantly influence the cell mass and docosahexaenoic acid content of i aurantiochytrium limacinum i strain pku sw8 |
| topic | thraustochytrids fatty acids docosahexaenoic acid cell mass monosaccharides salinity |
| url | https://www.mdpi.com/1660-3397/19/12/671 |
| work_keys_str_mv | AT xiaohongchen chemicalandphysicalcultureconditionssignificantlyinfluencethecellmassanddocosahexaenoicacidcontentofiaurantiochytriumlimacinumistrainpkusw8 AT biswarupsen chemicalandphysicalcultureconditionssignificantlyinfluencethecellmassanddocosahexaenoicacidcontentofiaurantiochytriumlimacinumistrainpkusw8 AT saizhang chemicalandphysicalcultureconditionssignificantlyinfluencethecellmassanddocosahexaenoicacidcontentofiaurantiochytriumlimacinumistrainpkusw8 AT mohanbai chemicalandphysicalcultureconditionssignificantlyinfluencethecellmassanddocosahexaenoicacidcontentofiaurantiochytriumlimacinumistrainpkusw8 AT yaodonghe chemicalandphysicalcultureconditionssignificantlyinfluencethecellmassanddocosahexaenoicacidcontentofiaurantiochytriumlimacinumistrainpkusw8 AT guangyiwang chemicalandphysicalcultureconditionssignificantlyinfluencethecellmassanddocosahexaenoicacidcontentofiaurantiochytriumlimacinumistrainpkusw8 |