Prostanoid-dependent bladder pain caused by proteinase-activated receptor-2 activation in mice: Involvement of TRPV1 and T-type Ca2+ channels
We studied the pronociceptive role of proteinase-activated receptor-2 (PAR2) in mouse bladder. In female mice, intravesical infusion of the PAR2-activating peptide, SLIGRL-amide (SL), caused delayed mechanical hypersensitivity in the lower abdomen, namely ‘referred hyperalgesia’, 6–24 h after the ad...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2018-01-01
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| Series: | Journal of Pharmacological Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861317302104 |
| _version_ | 1818452808876163072 |
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| author | Maho Tsubota Tomoka Ozaki Yuko Hayashi Yasumasa Okawa Ayaka Fujimura Fumiko Sekiguchi Hiroyuki Nishikawa Atsufumi Kawabata |
| author_facet | Maho Tsubota Tomoka Ozaki Yuko Hayashi Yasumasa Okawa Ayaka Fujimura Fumiko Sekiguchi Hiroyuki Nishikawa Atsufumi Kawabata |
| author_sort | Maho Tsubota |
| collection | DOAJ |
| description | We studied the pronociceptive role of proteinase-activated receptor-2 (PAR2) in mouse bladder. In female mice, intravesical infusion of the PAR2-activating peptide, SLIGRL-amide (SL), caused delayed mechanical hypersensitivity in the lower abdomen, namely ‘referred hyperalgesia’, 6–24 h after the administration. The PAR2-triggered referred hyperalgesia was prevented by indomethacin or a selective TRPV1 blocker, and restored by a T-type Ca2+ channel blocker. In human urothelial T24 cells, SL caused delayed prostaglandin E2 production and COX-2 upregulation. Our data suggest that luminal PAR2 stimulation in the bladder causes prostanoid-dependent referred hyperalgesia in mice, which involves the activation of TRPV1 and T-type Ca2+ channels. |
| first_indexed | 2024-12-14T21:28:59Z |
| format | Article |
| id | doaj.art-0a5272f9cc654d6eb4b4dd8bed133e87 |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-14T21:28:59Z |
| publishDate | 2018-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-0a5272f9cc654d6eb4b4dd8bed133e872022-12-21T22:46:43ZengElsevierJournal of Pharmacological Sciences1347-86132018-01-011361464910.1016/j.jphs.2017.12.007Prostanoid-dependent bladder pain caused by proteinase-activated receptor-2 activation in mice: Involvement of TRPV1 and T-type Ca2+ channelsMaho TsubotaTomoka OzakiYuko HayashiYasumasa OkawaAyaka FujimuraFumiko SekiguchiHiroyuki NishikawaAtsufumi KawabataWe studied the pronociceptive role of proteinase-activated receptor-2 (PAR2) in mouse bladder. In female mice, intravesical infusion of the PAR2-activating peptide, SLIGRL-amide (SL), caused delayed mechanical hypersensitivity in the lower abdomen, namely ‘referred hyperalgesia’, 6–24 h after the administration. The PAR2-triggered referred hyperalgesia was prevented by indomethacin or a selective TRPV1 blocker, and restored by a T-type Ca2+ channel blocker. In human urothelial T24 cells, SL caused delayed prostaglandin E2 production and COX-2 upregulation. Our data suggest that luminal PAR2 stimulation in the bladder causes prostanoid-dependent referred hyperalgesia in mice, which involves the activation of TRPV1 and T-type Ca2+ channels.http://www.sciencedirect.com/science/article/pii/S1347861317302104PAR2Prostaglandin E2Bladder pain |
| spellingShingle | Maho Tsubota Tomoka Ozaki Yuko Hayashi Yasumasa Okawa Ayaka Fujimura Fumiko Sekiguchi Hiroyuki Nishikawa Atsufumi Kawabata Prostanoid-dependent bladder pain caused by proteinase-activated receptor-2 activation in mice: Involvement of TRPV1 and T-type Ca2+ channels Journal of Pharmacological Sciences PAR2 Prostaglandin E2 Bladder pain |
| title | Prostanoid-dependent bladder pain caused by proteinase-activated receptor-2 activation in mice: Involvement of TRPV1 and T-type Ca2+ channels |
| title_full | Prostanoid-dependent bladder pain caused by proteinase-activated receptor-2 activation in mice: Involvement of TRPV1 and T-type Ca2+ channels |
| title_fullStr | Prostanoid-dependent bladder pain caused by proteinase-activated receptor-2 activation in mice: Involvement of TRPV1 and T-type Ca2+ channels |
| title_full_unstemmed | Prostanoid-dependent bladder pain caused by proteinase-activated receptor-2 activation in mice: Involvement of TRPV1 and T-type Ca2+ channels |
| title_short | Prostanoid-dependent bladder pain caused by proteinase-activated receptor-2 activation in mice: Involvement of TRPV1 and T-type Ca2+ channels |
| title_sort | prostanoid dependent bladder pain caused by proteinase activated receptor 2 activation in mice involvement of trpv1 and t type ca2 channels |
| topic | PAR2 Prostaglandin E2 Bladder pain |
| url | http://www.sciencedirect.com/science/article/pii/S1347861317302104 |
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