Outbreak investigation of acute febrile illness from the Himalayan foothills: Solving the puzzle of fever
In September 2022, Panchkula Civil Hospital reported an outbreak of acute febrile illness (AFI) in Pinjore, located in the Himalayan foothills, Haryana, North India. There was an upsurge of fever cases. Blood samples were taken from suspected patients (n = 58) with AFI and subjected to serology of d...
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| Format: | Article |
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2023.1159377/full |
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| author | Prakasini Satapathy Kapil Goel Vikrant Sharma Subhabrata Sarkar Mannat Kang Shefali Dhingra Ishani Bora Kanwalpreet Kaur Neeraj Arora Arun Aggarwal Radha Kanta Ratho |
| author_facet | Prakasini Satapathy Kapil Goel Vikrant Sharma Subhabrata Sarkar Mannat Kang Shefali Dhingra Ishani Bora Kanwalpreet Kaur Neeraj Arora Arun Aggarwal Radha Kanta Ratho |
| author_sort | Prakasini Satapathy |
| collection | DOAJ |
| description | In September 2022, Panchkula Civil Hospital reported an outbreak of acute febrile illness (AFI) in Pinjore, located in the Himalayan foothills, Haryana, North India. There was an upsurge of fever cases. Blood samples were taken from suspected patients (n = 58) with AFI and subjected to serology of dengue, chikungunya, Japanese encephalitis, leptospira and scrub typhus. The samples were also screened for West Nile & Zika virus RNA using real-time PCR. Viral strains were characterized by sequencing. Of the 58 cases of AFI, Dengue could be identified in 45 (77.58%) followed by JE and Chikungunya in 2 cases each (3.44%), respectively. Among Dengue positive cases, 44 had monoinfection (97.77%) and 1 patient had dengue and JE. None were positive for Zika, West Nile, Scrub typhus, and Leptospira with the testing protocol. Four patients developed dengue with warning signs, such as abdominal pain in one patient and recurrent vomiting in the remaining three. The dengue serotype could be determined in 17 samples and revealed serotype 2. Molecular evolution analysis based on the complete envelope gene revealed that all DENV-2 strains (n = 13) circulated in the outbreak area belonged to the DENV-2 cosmopoliton genotype. In the early stages of infection, relying only on clinical manifestations is ineffective, so both molecular and serological assays along with clinical diagnosis are noteworthy for determining the aetiology of AFI. |
| first_indexed | 2024-03-11T15:37:48Z |
| format | Article |
| id | doaj.art-0a561968c78449f4a8a7e626a7909489 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-03-11T15:37:48Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-0a561968c78449f4a8a7e626a79094892023-10-26T13:18:16ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122023-10-011410.3389/fphar.2023.11593771159377Outbreak investigation of acute febrile illness from the Himalayan foothills: Solving the puzzle of feverPrakasini Satapathy0Kapil Goel1Vikrant Sharma2Subhabrata Sarkar3Mannat Kang4Shefali Dhingra5Ishani Bora6Kanwalpreet Kaur7Neeraj Arora8Arun Aggarwal9Radha Kanta Ratho10Department of Virology, Postgraduate Institute of Medical Education and Research, Chandigarh, IndiaDepartment of Community Medicine & SPH, PGIMER, Chandigarh, IndiaDepartment of Virology, Postgraduate Institute of Medical Education and Research, Chandigarh, IndiaDepartment of Virology, Postgraduate Institute of Medical Education and Research, Chandigarh, IndiaDepartment of Virology, Postgraduate Institute of Medical Education and Research, Chandigarh, IndiaDepartment of Virology, Postgraduate Institute of Medical Education and Research, Chandigarh, IndiaDepartment of Virology, Postgraduate Institute of Medical Education and Research, Chandigarh, IndiaDepartment of Virology, Postgraduate Institute of Medical Education and Research, Chandigarh, IndiaLab Medicine, Civil Hospital, Panchkula, IndiaDepartment of Community Medicine & SPH, PGIMER, Chandigarh, IndiaDepartment of Virology, Postgraduate Institute of Medical Education and Research, Chandigarh, IndiaIn September 2022, Panchkula Civil Hospital reported an outbreak of acute febrile illness (AFI) in Pinjore, located in the Himalayan foothills, Haryana, North India. There was an upsurge of fever cases. Blood samples were taken from suspected patients (n = 58) with AFI and subjected to serology of dengue, chikungunya, Japanese encephalitis, leptospira and scrub typhus. The samples were also screened for West Nile & Zika virus RNA using real-time PCR. Viral strains were characterized by sequencing. Of the 58 cases of AFI, Dengue could be identified in 45 (77.58%) followed by JE and Chikungunya in 2 cases each (3.44%), respectively. Among Dengue positive cases, 44 had monoinfection (97.77%) and 1 patient had dengue and JE. None were positive for Zika, West Nile, Scrub typhus, and Leptospira with the testing protocol. Four patients developed dengue with warning signs, such as abdominal pain in one patient and recurrent vomiting in the remaining three. The dengue serotype could be determined in 17 samples and revealed serotype 2. Molecular evolution analysis based on the complete envelope gene revealed that all DENV-2 strains (n = 13) circulated in the outbreak area belonged to the DENV-2 cosmopoliton genotype. In the early stages of infection, relying only on clinical manifestations is ineffective, so both molecular and serological assays along with clinical diagnosis are noteworthy for determining the aetiology of AFI.https://www.frontiersin.org/articles/10.3389/fphar.2023.1159377/fullacute febrile illnessdengue feverDENV-2cosmopoliton genotypeoutbreak |
| spellingShingle | Prakasini Satapathy Kapil Goel Vikrant Sharma Subhabrata Sarkar Mannat Kang Shefali Dhingra Ishani Bora Kanwalpreet Kaur Neeraj Arora Arun Aggarwal Radha Kanta Ratho Outbreak investigation of acute febrile illness from the Himalayan foothills: Solving the puzzle of fever Frontiers in Pharmacology acute febrile illness dengue fever DENV-2 cosmopoliton genotype outbreak |
| title | Outbreak investigation of acute febrile illness from the Himalayan foothills: Solving the puzzle of fever |
| title_full | Outbreak investigation of acute febrile illness from the Himalayan foothills: Solving the puzzle of fever |
| title_fullStr | Outbreak investigation of acute febrile illness from the Himalayan foothills: Solving the puzzle of fever |
| title_full_unstemmed | Outbreak investigation of acute febrile illness from the Himalayan foothills: Solving the puzzle of fever |
| title_short | Outbreak investigation of acute febrile illness from the Himalayan foothills: Solving the puzzle of fever |
| title_sort | outbreak investigation of acute febrile illness from the himalayan foothills solving the puzzle of fever |
| topic | acute febrile illness dengue fever DENV-2 cosmopoliton genotype outbreak |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2023.1159377/full |
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