Detection of the <i>agr</i> System and Resistance to Antimicrobials in Biofilm-Producing <i>S. epidermidis</i>
The ability of <i>Staphylococcus epidermidis</i> to produce virulence factors, such as biofilm, added to its increased resistance to antimicrobials can cause infections that are difficult to treat. Many staphylococcal virulence factors are under the control of the accessory gene regulato...
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MDPI AG
2020-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/23/5715 |
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| author | Valéria Cataneli Pereira Luiza Pinheiro-Hubinger Adilson de Oliveira Danilo Flávio Moraes Riboli Katheryne Benini Martins Letícia Calixto Romero Maria de Lourdes Ribeiro de Souza da Cunha |
| author_facet | Valéria Cataneli Pereira Luiza Pinheiro-Hubinger Adilson de Oliveira Danilo Flávio Moraes Riboli Katheryne Benini Martins Letícia Calixto Romero Maria de Lourdes Ribeiro de Souza da Cunha |
| author_sort | Valéria Cataneli Pereira |
| collection | DOAJ |
| description | The ability of <i>Staphylococcus epidermidis</i> to produce virulence factors, such as biofilm, added to its increased resistance to antimicrobials can cause infections that are difficult to treat. Many staphylococcal virulence factors are under the control of the accessory gene regulator (<i>agr</i>). The objective of this study was to establish the <i>agr</i> locus and susceptibility of biofilm-producing <i>S. epidermidis</i> specimens to antimicrobial agents, through PCR reactions, reverse transcription polymerase chain reaction (RT-PCR), and the determination of minimum inhibitory concentration (MIC), and to analyze the clonal profile of 300 strains isolated from blood culture specimens from inpatients at a University Hospital in Brazil, over a 20-year period by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) techniques. The <i>ica</i> operon expression was shown in 83.6% strains, <i>bhp</i> gene in 11.5%, and <i>aap</i> gene in 32.8%. Oxacillin resistance was detected in 90.1%, while 4.9% showed tigecycline resistance, and intermediate resistance to quinupristin/dalfopristin was identified in 0.4%. Clonal profile determination showed 11 clusters, with the ST2 type determined as the major cluster. The <i>S. epidermidis</i> biofilm producer demonstrated a predominance of <i>agr</i> I locus, oxacillin resistance, and SCC<i>mec</i> III as well as the potential dissemination of pathogenic clones in hospital settings over long periods. |
| first_indexed | 2024-03-10T14:20:03Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T14:20:03Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-0a570f8fe5934b2295d1fea49746ab8b2023-11-20T23:24:43ZengMDPI AGMolecules1420-30492020-12-012523571510.3390/molecules25235715Detection of the <i>agr</i> System and Resistance to Antimicrobials in Biofilm-Producing <i>S. epidermidis</i>Valéria Cataneli Pereira0Luiza Pinheiro-Hubinger1Adilson de Oliveira2Danilo Flávio Moraes Riboli3Katheryne Benini Martins4Letícia Calixto Romero5Maria de Lourdes Ribeiro de Souza da Cunha6Sector of Microbiology and Immunology, Department of Chemical and Biological Sciences, Institute of Biosciences, UNESP—University Estadual Paulista, Botucatu CEP 18618-689, São Paulo, BrazilSector of Microbiology and Immunology, Department of Chemical and Biological Sciences, Institute of Biosciences, UNESP—University Estadual Paulista, Botucatu CEP 18618-689, São Paulo, BrazilSector of Microbiology and Immunology, Department of Chemical and Biological Sciences, Institute of Biosciences, UNESP—University Estadual Paulista, Botucatu CEP 18618-689, São Paulo, BrazilSector of Microbiology and Immunology, Department of Chemical and Biological Sciences, Institute of Biosciences, UNESP—University Estadual Paulista, Botucatu CEP 18618-689, São Paulo, BrazilSector of Microbiology and Immunology, Department of Chemical and Biological Sciences, Institute of Biosciences, UNESP—University Estadual Paulista, Botucatu CEP 18618-689, São Paulo, BrazilSector of Microbiology and Immunology, Department of Chemical and Biological Sciences, Institute of Biosciences, UNESP—University Estadual Paulista, Botucatu CEP 18618-689, São Paulo, BrazilSector of Microbiology and Immunology, Department of Chemical and Biological Sciences, Institute of Biosciences, UNESP—University Estadual Paulista, Botucatu CEP 18618-689, São Paulo, BrazilThe ability of <i>Staphylococcus epidermidis</i> to produce virulence factors, such as biofilm, added to its increased resistance to antimicrobials can cause infections that are difficult to treat. Many staphylococcal virulence factors are under the control of the accessory gene regulator (<i>agr</i>). The objective of this study was to establish the <i>agr</i> locus and susceptibility of biofilm-producing <i>S. epidermidis</i> specimens to antimicrobial agents, through PCR reactions, reverse transcription polymerase chain reaction (RT-PCR), and the determination of minimum inhibitory concentration (MIC), and to analyze the clonal profile of 300 strains isolated from blood culture specimens from inpatients at a University Hospital in Brazil, over a 20-year period by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) techniques. The <i>ica</i> operon expression was shown in 83.6% strains, <i>bhp</i> gene in 11.5%, and <i>aap</i> gene in 32.8%. Oxacillin resistance was detected in 90.1%, while 4.9% showed tigecycline resistance, and intermediate resistance to quinupristin/dalfopristin was identified in 0.4%. Clonal profile determination showed 11 clusters, with the ST2 type determined as the major cluster. The <i>S. epidermidis</i> biofilm producer demonstrated a predominance of <i>agr</i> I locus, oxacillin resistance, and SCC<i>mec</i> III as well as the potential dissemination of pathogenic clones in hospital settings over long periods.https://www.mdpi.com/1420-3049/25/23/5715oxacillin resistanceST2<i>agr</i> locusbiofilmantimicrobial resistance |
| spellingShingle | Valéria Cataneli Pereira Luiza Pinheiro-Hubinger Adilson de Oliveira Danilo Flávio Moraes Riboli Katheryne Benini Martins Letícia Calixto Romero Maria de Lourdes Ribeiro de Souza da Cunha Detection of the <i>agr</i> System and Resistance to Antimicrobials in Biofilm-Producing <i>S. epidermidis</i> Molecules oxacillin resistance ST2 <i>agr</i> locus biofilm antimicrobial resistance |
| title | Detection of the <i>agr</i> System and Resistance to Antimicrobials in Biofilm-Producing <i>S. epidermidis</i> |
| title_full | Detection of the <i>agr</i> System and Resistance to Antimicrobials in Biofilm-Producing <i>S. epidermidis</i> |
| title_fullStr | Detection of the <i>agr</i> System and Resistance to Antimicrobials in Biofilm-Producing <i>S. epidermidis</i> |
| title_full_unstemmed | Detection of the <i>agr</i> System and Resistance to Antimicrobials in Biofilm-Producing <i>S. epidermidis</i> |
| title_short | Detection of the <i>agr</i> System and Resistance to Antimicrobials in Biofilm-Producing <i>S. epidermidis</i> |
| title_sort | detection of the i agr i system and resistance to antimicrobials in biofilm producing i s epidermidis i |
| topic | oxacillin resistance ST2 <i>agr</i> locus biofilm antimicrobial resistance |
| url | https://www.mdpi.com/1420-3049/25/23/5715 |
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