Accuracy of SD Malaria Ag P.f/Pan® as a rapid diagnostic test in French Amazonia
Abstract Background French Guiana (FG) is a French overseas territory where malaria is endemic. The current incidence rate is 0.74‰ inhabitants, and Plasmodium vivax is widely predominating even though Plasmodium falciparum is still present due to imported cases mainly from Africa. In FG, rapid diag...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2021-09-01
|
| Series: | Malaria Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12936-021-03902-z |
| _version_ | 1819101651927040000 |
|---|---|
| author | Jean Marc Pujo Stéphanie Houcke Sarah Lemmonier Patrick Portecop Alexis Frémery Denis Blanchet Felix Djossou Hatem Kallel Magalie Demar |
| author_facet | Jean Marc Pujo Stéphanie Houcke Sarah Lemmonier Patrick Portecop Alexis Frémery Denis Blanchet Felix Djossou Hatem Kallel Magalie Demar |
| author_sort | Jean Marc Pujo |
| collection | DOAJ |
| description | Abstract Background French Guiana (FG) is a French overseas territory where malaria is endemic. The current incidence rate is 0.74‰ inhabitants, and Plasmodium vivax is widely predominating even though Plasmodium falciparum is still present due to imported cases mainly from Africa. In FG, rapid diagnostic test (SD Malaria Ag P.f/Pan®) is based on the detection of pan-pLDH, PfHRP2, and PfHRP3 antigens, while in South America, the share of deletion of PfHRP2 gene is significantly increasing. Accordingly, the study questions the reliability of RDTs in the Amazonian context. Methods The study is retrospective. It is conducted over 4 years and analysed 12,880 rapid diagnostic tests (RDTs) compared to concomitant Blood Film Tests (BFTs) sampled for malaria diagnosis. Results The global assessment of the accuracy of SD Malaria Ag P.f/Pan® in the diagnostic of malaria shows both Positive and Negative Predictive Values (PPV and NPV) higher than 95%, except for PPV in the diagnosis of malaria to P. falciparum (88%). Overall, the concordance rate between RDT and BFT (positive/positive; negative/negative) was 99.5%. The PPV of the RDT in the follow-up of patients diagnosed with P. falciparum was the lowest during the first 28 days. The PPV of the RDT in the follow-up of patients diagnosed with P. vivax was the lowest during the first 21 days. The global sensitivity of SD Malaria Ag P.f/Pan® test was, on average, 96% (88.2–100) for P. falciparum and 93% (90.6–94.2) for P. vivax. The global specificity was 99.8% (99.5–100) for all included species. Conclusion SD Malaria Ag P.f/Pan® is a reliable rapid test used for the first-line diagnosis in remote healthcare centres. The test results should be interpreted in the light of patient’s recent medical history and the date of arrival to FG. |
| first_indexed | 2024-12-22T01:22:04Z |
| format | Article |
| id | doaj.art-0a59a3c856de442fae7fe78a7e6d00b3 |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-12-22T01:22:04Z |
| publishDate | 2021-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-0a59a3c856de442fae7fe78a7e6d00b32022-12-21T18:43:42ZengBMCMalaria Journal1475-28752021-09-012011710.1186/s12936-021-03902-zAccuracy of SD Malaria Ag P.f/Pan® as a rapid diagnostic test in French AmazoniaJean Marc Pujo0Stéphanie Houcke1Sarah Lemmonier2Patrick Portecop3Alexis Frémery4Denis Blanchet5Felix Djossou6Hatem Kallel7Magalie Demar8Emergency Department, Cayenne General HospitalIntensive Care Unit, Cayenne General HospitalEmergency Department, Cayenne General HospitalEmergency Department, Guadeloupe University HospitalEmergency Department, Cayenne General HospitalAcademic Laboratory of Parasitology-Mycology, Cayenne General HospitalInfectious and Tropical Diseases Unit, Cayenne General HospitalIntensive Care Unit, Cayenne General HospitalAcademic Laboratory of Parasitology-Mycology, Cayenne General HospitalAbstract Background French Guiana (FG) is a French overseas territory where malaria is endemic. The current incidence rate is 0.74‰ inhabitants, and Plasmodium vivax is widely predominating even though Plasmodium falciparum is still present due to imported cases mainly from Africa. In FG, rapid diagnostic test (SD Malaria Ag P.f/Pan®) is based on the detection of pan-pLDH, PfHRP2, and PfHRP3 antigens, while in South America, the share of deletion of PfHRP2 gene is significantly increasing. Accordingly, the study questions the reliability of RDTs in the Amazonian context. Methods The study is retrospective. It is conducted over 4 years and analysed 12,880 rapid diagnostic tests (RDTs) compared to concomitant Blood Film Tests (BFTs) sampled for malaria diagnosis. Results The global assessment of the accuracy of SD Malaria Ag P.f/Pan® in the diagnostic of malaria shows both Positive and Negative Predictive Values (PPV and NPV) higher than 95%, except for PPV in the diagnosis of malaria to P. falciparum (88%). Overall, the concordance rate between RDT and BFT (positive/positive; negative/negative) was 99.5%. The PPV of the RDT in the follow-up of patients diagnosed with P. falciparum was the lowest during the first 28 days. The PPV of the RDT in the follow-up of patients diagnosed with P. vivax was the lowest during the first 21 days. The global sensitivity of SD Malaria Ag P.f/Pan® test was, on average, 96% (88.2–100) for P. falciparum and 93% (90.6–94.2) for P. vivax. The global specificity was 99.8% (99.5–100) for all included species. Conclusion SD Malaria Ag P.f/Pan® is a reliable rapid test used for the first-line diagnosis in remote healthcare centres. The test results should be interpreted in the light of patient’s recent medical history and the date of arrival to FG.https://doi.org/10.1186/s12936-021-03902-zMalariaRapid diagnosis testsAccuracyPfHRP2 gene deletion |
| spellingShingle | Jean Marc Pujo Stéphanie Houcke Sarah Lemmonier Patrick Portecop Alexis Frémery Denis Blanchet Felix Djossou Hatem Kallel Magalie Demar Accuracy of SD Malaria Ag P.f/Pan® as a rapid diagnostic test in French Amazonia Malaria Journal Malaria Rapid diagnosis tests Accuracy PfHRP2 gene deletion |
| title | Accuracy of SD Malaria Ag P.f/Pan® as a rapid diagnostic test in French Amazonia |
| title_full | Accuracy of SD Malaria Ag P.f/Pan® as a rapid diagnostic test in French Amazonia |
| title_fullStr | Accuracy of SD Malaria Ag P.f/Pan® as a rapid diagnostic test in French Amazonia |
| title_full_unstemmed | Accuracy of SD Malaria Ag P.f/Pan® as a rapid diagnostic test in French Amazonia |
| title_short | Accuracy of SD Malaria Ag P.f/Pan® as a rapid diagnostic test in French Amazonia |
| title_sort | accuracy of sd malaria ag p f pan r as a rapid diagnostic test in french amazonia |
| topic | Malaria Rapid diagnosis tests Accuracy PfHRP2 gene deletion |
| url | https://doi.org/10.1186/s12936-021-03902-z |
| work_keys_str_mv | AT jeanmarcpujo accuracyofsdmalariaagpfpanasarapiddiagnostictestinfrenchamazonia AT stephaniehoucke accuracyofsdmalariaagpfpanasarapiddiagnostictestinfrenchamazonia AT sarahlemmonier accuracyofsdmalariaagpfpanasarapiddiagnostictestinfrenchamazonia AT patrickportecop accuracyofsdmalariaagpfpanasarapiddiagnostictestinfrenchamazonia AT alexisfremery accuracyofsdmalariaagpfpanasarapiddiagnostictestinfrenchamazonia AT denisblanchet accuracyofsdmalariaagpfpanasarapiddiagnostictestinfrenchamazonia AT felixdjossou accuracyofsdmalariaagpfpanasarapiddiagnostictestinfrenchamazonia AT hatemkallel accuracyofsdmalariaagpfpanasarapiddiagnostictestinfrenchamazonia AT magaliedemar accuracyofsdmalariaagpfpanasarapiddiagnostictestinfrenchamazonia |