An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels
A simple protocol is described for the silver staining of polyacrylamide gradient gels used for the separation of restriction fragments of kinetoplast DNA [schizodeme analysis of trypanosomatids (Morel et al., 1980)]. The method overcomes the problems of non-uniform staining and strong background co...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
1990-03-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000100017 |
| _version_ | 1797704704506986496 |
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| author | Antonio M. Gonçalves Nedia S. Nehme Carlos M. Morel |
| author_facet | Antonio M. Gonçalves Nedia S. Nehme Carlos M. Morel |
| author_sort | Antonio M. Gonçalves |
| collection | DOAJ |
| description | A simple protocol is described for the silver staining of polyacrylamide gradient gels used for the separation of restriction fragments of kinetoplast DNA [schizodeme analysis of trypanosomatids (Morel et al., 1980)]. The method overcomes the problems of non-uniform staining and strong background color which are frequently encountered when conventional protocols for silver staining of linear gels. The method described has proven to be of general applicability for DNA, RNA and protein separations in gradient gels. |
| first_indexed | 2024-03-12T05:24:23Z |
| format | Article |
| id | doaj.art-0a5ba176215548eb84908b65af188a1e |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T05:24:23Z |
| publishDate | 1990-03-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-0a5ba176215548eb84908b65af188a1e2023-09-03T07:24:46ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601990-03-0185110110610.1590/S0074-02761990000100017An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gelsAntonio M. GonçalvesNedia S. NehmeCarlos M. MorelA simple protocol is described for the silver staining of polyacrylamide gradient gels used for the separation of restriction fragments of kinetoplast DNA [schizodeme analysis of trypanosomatids (Morel et al., 1980)]. The method overcomes the problems of non-uniform staining and strong background color which are frequently encountered when conventional protocols for silver staining of linear gels. The method described has proven to be of general applicability for DNA, RNA and protein separations in gradient gels.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000100017silver stainingschizodeme analysisDNARNAproteintrypanosomatids |
| spellingShingle | Antonio M. Gonçalves Nedia S. Nehme Carlos M. Morel An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels Memorias do Instituto Oswaldo Cruz silver staining schizodeme analysis DNA RNA protein trypanosomatids |
| title | An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels |
| title_full | An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels |
| title_fullStr | An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels |
| title_full_unstemmed | An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels |
| title_short | An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels |
| title_sort | improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels |
| topic | silver staining schizodeme analysis DNA RNA protein trypanosomatids |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000100017 |
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