Fungi with potential probiotic properties isolated from Fuzhuan brick tea
Several of the fungal species associated with Fuzhuan brick tea (FBT) are considered as potential probiotics, but few studies have investigated the probiotic properties of these fungi. Here, we isolated 18 fungal strains from two types of FBT and identified these strains based on internal transcribe...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tsinghua University Press
2022-05-01
|
| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453021001543 |
| _version_ | 1797728177184833536 |
|---|---|
| author | Xin Wang Yuanyuan Cui Chaowei Sang Bin Wang Yirong Yuan Lin Liu Yahong Yuan Tianli Yue |
| author_facet | Xin Wang Yuanyuan Cui Chaowei Sang Bin Wang Yirong Yuan Lin Liu Yahong Yuan Tianli Yue |
| author_sort | Xin Wang |
| collection | DOAJ |
| description | Several of the fungal species associated with Fuzhuan brick tea (FBT) are considered as potential probiotics, but few studies have investigated the probiotic properties of these fungi. Here, we isolated 18 fungal strains from two types of FBT and identified these strains based on internal transcribed spacer (ITS) fragment sequence similarity to reference strains (sequence similarity > 98%). Of the 18 strains, 10 tolerated simulated human digestive conditions for sufficient periods in vitro: pH 2-3, 0.3%-0.5% (m/V) bile salts, and artificial gastrointestinal juices. We then measured the antimicrobial activity of the remaining 10 strains against 5 enteropathogenic bacteria and tested the bacteriostatic effects of the thalli and fermentation broth extracts. Of the 6 strains with strong bacteriostatic effects, we eliminated Eurotium cristatum S-9 due to its low hydrophobicity of (26.12 ± 0.35)%. Finally, 2 exhibited good adhesion abilities to human cells (> 100%). Notably, 2 strains can survive in vivo, because they can be isolated from C57BL/6 mice feces. Thus, 2 strains, Aspergillus cristatus H-1 and A. cristatus H-5, are herein identified as promising candidate probiotic strains. It may be put forward a novel research focus on evaluating potential probiotic fungi from FBT. |
| first_indexed | 2024-03-12T11:10:06Z |
| format | Article |
| id | doaj.art-0a6080a11c3a4372b228f323f8c04979 |
| institution | Directory Open Access Journal |
| issn | 2213-4530 |
| language | English |
| last_indexed | 2024-03-12T11:10:06Z |
| publishDate | 2022-05-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj.art-0a6080a11c3a4372b228f323f8c049792023-09-02T03:00:24ZengTsinghua University PressFood Science and Human Wellness2213-45302022-05-01113686696Fungi with potential probiotic properties isolated from Fuzhuan brick teaXin Wang0Yuanyuan Cui1Chaowei Sang2Bin Wang3Yirong Yuan4Lin Liu5Yahong Yuan6Tianli Yue7College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China; Laboratory of Quality & Safety Risk Assessment for Agro-products (YangLing), Ministry of Agriculture, Yangling 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, China; Laboratory of Quality & Safety Risk Assessment for Agro-products (YangLing), Ministry of Agriculture, Yangling 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, China; Laboratory of Quality & Safety Risk Assessment for Agro-products (YangLing), Ministry of Agriculture, Yangling 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, China; Laboratory of Quality & Safety Risk Assessment for Agro-products (YangLing), Ministry of Agriculture, Yangling 712100, China; Corresponding authors at: Northwest A&F University, Yangling 712100, China.College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China; Laboratory of Quality & Safety Risk Assessment for Agro-products (YangLing), Ministry of Agriculture, Yangling 712100, China; College of Food Science and Technology, Northwest University, Xi’an 710069, China; Corresponding authors at: Northwest A&F University, Yangling 712100, China.Several of the fungal species associated with Fuzhuan brick tea (FBT) are considered as potential probiotics, but few studies have investigated the probiotic properties of these fungi. Here, we isolated 18 fungal strains from two types of FBT and identified these strains based on internal transcribed spacer (ITS) fragment sequence similarity to reference strains (sequence similarity > 98%). Of the 18 strains, 10 tolerated simulated human digestive conditions for sufficient periods in vitro: pH 2-3, 0.3%-0.5% (m/V) bile salts, and artificial gastrointestinal juices. We then measured the antimicrobial activity of the remaining 10 strains against 5 enteropathogenic bacteria and tested the bacteriostatic effects of the thalli and fermentation broth extracts. Of the 6 strains with strong bacteriostatic effects, we eliminated Eurotium cristatum S-9 due to its low hydrophobicity of (26.12 ± 0.35)%. Finally, 2 exhibited good adhesion abilities to human cells (> 100%). Notably, 2 strains can survive in vivo, because they can be isolated from C57BL/6 mice feces. Thus, 2 strains, Aspergillus cristatus H-1 and A. cristatus H-5, are herein identified as promising candidate probiotic strains. It may be put forward a novel research focus on evaluating potential probiotic fungi from FBT.http://www.sciencedirect.com/science/article/pii/S2213453021001543Fuzhuan brick teaFungiCandidate probioticsProbiotic propertiesBacteriostatic activityAdhesion capacity |
| spellingShingle | Xin Wang Yuanyuan Cui Chaowei Sang Bin Wang Yirong Yuan Lin Liu Yahong Yuan Tianli Yue Fungi with potential probiotic properties isolated from Fuzhuan brick tea Food Science and Human Wellness Fuzhuan brick tea Fungi Candidate probiotics Probiotic properties Bacteriostatic activity Adhesion capacity |
| title | Fungi with potential probiotic properties isolated from Fuzhuan brick tea |
| title_full | Fungi with potential probiotic properties isolated from Fuzhuan brick tea |
| title_fullStr | Fungi with potential probiotic properties isolated from Fuzhuan brick tea |
| title_full_unstemmed | Fungi with potential probiotic properties isolated from Fuzhuan brick tea |
| title_short | Fungi with potential probiotic properties isolated from Fuzhuan brick tea |
| title_sort | fungi with potential probiotic properties isolated from fuzhuan brick tea |
| topic | Fuzhuan brick tea Fungi Candidate probiotics Probiotic properties Bacteriostatic activity Adhesion capacity |
| url | http://www.sciencedirect.com/science/article/pii/S2213453021001543 |
| work_keys_str_mv | AT xinwang fungiwithpotentialprobioticpropertiesisolatedfromfuzhuanbricktea AT yuanyuancui fungiwithpotentialprobioticpropertiesisolatedfromfuzhuanbricktea AT chaoweisang fungiwithpotentialprobioticpropertiesisolatedfromfuzhuanbricktea AT binwang fungiwithpotentialprobioticpropertiesisolatedfromfuzhuanbricktea AT yirongyuan fungiwithpotentialprobioticpropertiesisolatedfromfuzhuanbricktea AT linliu fungiwithpotentialprobioticpropertiesisolatedfromfuzhuanbricktea AT yahongyuan fungiwithpotentialprobioticpropertiesisolatedfromfuzhuanbricktea AT tianliyue fungiwithpotentialprobioticpropertiesisolatedfromfuzhuanbricktea |