OPTIMISATION OF Bacillus amyloliquefaciens FE-K1 EXTRACELLULAR PEPTIDASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY
In this study, it was aimed to optimise the extracellular peptidase production of Bacillus amyloliquefaciens FE-K1, previously isolated from ropy wholemeal bread, by using response surface methodology (RSM) based on central composite design (CCD). The temperature (20-45°C), initial pH of the enzyme...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Trakya University
2018-10-01
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| Series: | Trakya University Journal of Natural Sciences |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/en/pub/trkjnat/issue/39729/439763 |
| _version_ | 1797919623288455168 |
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| author | Muharrem Certel Mehmet İnan Fundagül Erem |
| author_facet | Muharrem Certel Mehmet İnan Fundagül Erem |
| author_sort | Muharrem Certel |
| collection | DOAJ |
| description | In this study, it was aimed to optimise the extracellular peptidase production of Bacillus amyloliquefaciens FE-K1, previously isolated from ropy wholemeal bread, by using response surface methodology (RSM) based on central composite design (CCD). The temperature (20-45°C), initial pH of the enzyme production medium (pH 5-9) and inoculation level (1-5%, v/v) were used as the factors for RSM, and the fermentation time was determined for each trial separately. Results showed that the optimum peptidase production occurred at 33.4°C, pH 6.62 and 2.3% inoculation. It was determined that the fermentation time was only 7h, the crude enzyme had a peptidase activity of 49.17U/mL and a specific activity of 504.77U/mg under the optimised conditions. |
| first_indexed | 2024-04-10T13:48:27Z |
| format | Article |
| id | doaj.art-0a61423cf8574d9e88f2d2f86519a041 |
| institution | Directory Open Access Journal |
| issn | 2147-0294 2528-9691 |
| language | English |
| last_indexed | 2024-04-10T13:48:27Z |
| publishDate | 2018-10-01 |
| publisher | Trakya University |
| record_format | Article |
| series | Trakya University Journal of Natural Sciences |
| spelling | doaj.art-0a61423cf8574d9e88f2d2f86519a0412023-02-15T16:10:49ZengTrakya UniversityTrakya University Journal of Natural Sciences2147-02942528-96912018-10-0119215917369OPTIMISATION OF Bacillus amyloliquefaciens FE-K1 EXTRACELLULAR PEPTIDASE PRODUCTION BY RESPONSE SURFACE METHODOLOGYMuharrem Certel0Mehmet İnan1Fundagül Erem2AKDENİZ ÜNİVERSİTESİ, MÜHENDİSLİK FAKÜLTESİAKDENİZ ÜNİVERSİTESİ, MÜHENDİSLİK FAKÜLTESİBÜLENT ECEVİT ÜNİVERSİTESİ, MÜHENDİSLİK FAKÜLTESİIn this study, it was aimed to optimise the extracellular peptidase production of Bacillus amyloliquefaciens FE-K1, previously isolated from ropy wholemeal bread, by using response surface methodology (RSM) based on central composite design (CCD). The temperature (20-45°C), initial pH of the enzyme production medium (pH 5-9) and inoculation level (1-5%, v/v) were used as the factors for RSM, and the fermentation time was determined for each trial separately. Results showed that the optimum peptidase production occurred at 33.4°C, pH 6.62 and 2.3% inoculation. It was determined that the fermentation time was only 7h, the crude enzyme had a peptidase activity of 49.17U/mL and a specific activity of 504.77U/mg under the optimised conditions.https://dergipark.org.tr/en/pub/trkjnat/issue/39729/439763bacilluscentral composite designpeptidaseresponse surface methodologybacilluscentral composite designpeptidaseresponse surface methodology |
| spellingShingle | Muharrem Certel Mehmet İnan Fundagül Erem OPTIMISATION OF Bacillus amyloliquefaciens FE-K1 EXTRACELLULAR PEPTIDASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY Trakya University Journal of Natural Sciences bacillus central composite design peptidase response surface methodology bacillus central composite design peptidase response surface methodology |
| title | OPTIMISATION OF Bacillus amyloliquefaciens FE-K1 EXTRACELLULAR PEPTIDASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY |
| title_full | OPTIMISATION OF Bacillus amyloliquefaciens FE-K1 EXTRACELLULAR PEPTIDASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY |
| title_fullStr | OPTIMISATION OF Bacillus amyloliquefaciens FE-K1 EXTRACELLULAR PEPTIDASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY |
| title_full_unstemmed | OPTIMISATION OF Bacillus amyloliquefaciens FE-K1 EXTRACELLULAR PEPTIDASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY |
| title_short | OPTIMISATION OF Bacillus amyloliquefaciens FE-K1 EXTRACELLULAR PEPTIDASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY |
| title_sort | optimisation of bacillus amyloliquefaciens fe k1 extracellular peptidase production by response surface methodology |
| topic | bacillus central composite design peptidase response surface methodology bacillus central composite design peptidase response surface methodology |
| url | https://dergipark.org.tr/en/pub/trkjnat/issue/39729/439763 |
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