Discrepancy of Beta-Hydroxybutyrate Measurements between a Blood Meter and GC-MS Methods in Healthy Humans
Ketone salt (KS) supplementation induces temporary nutritional ketosis to achieve potential exercise performance and health benefits. Racemic KS includes both D/L isomers of β-hydroxybutyrate, yet commercially available measurement devices (i.e., blood meters) only measure the D variant. The aim of...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Muscles |
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| Online Access: | https://www.mdpi.com/2813-0413/2/4/25 |
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| author | Angelia Maleah Holland-Winkler Andrew R. Moore Jenna K. Ansley Noah A. Fritz Ilya Bederman |
| author_facet | Angelia Maleah Holland-Winkler Andrew R. Moore Jenna K. Ansley Noah A. Fritz Ilya Bederman |
| author_sort | Angelia Maleah Holland-Winkler |
| collection | DOAJ |
| description | Ketone salt (KS) supplementation induces temporary nutritional ketosis to achieve potential exercise performance and health benefits. Racemic KS includes both D/L isomers of β-hydroxybutyrate, yet commercially available measurement devices (i.e., blood meters) only measure the D variant. The aim of this study was to investigate the efficacy of a blood meter to measure serum β-hydroxybutyrate in comparison with gas chromatography–mass spectrometry (GC-MS) before and 30 min after consuming a placebo or racemic KS. In this triple-blinded cross-over study, 16 healthy adults were administered either a placebo or KS drink, and the circulating β-hydroxybutyrate concentration was measured at baseline (PRE) and 30 min following consumption (POST) using a blood ketone meter and by GC-MS. Compared to the placebo, both GC-MS and the blood meter obtained significantly greater β-hydroxybutyrate levels from PRE to POST time-points after consuming KS. Additionally, GC-MS results showed significantly higher levels of β-hydroxybutyrate with both the placebo and KS at PRE and POST time-points, as compared to the blood meter. These results indicate that (1) even in the absence of KS, the blood meter yields significantly lower β-hydroxybutyrate values than GC-MS, and (2) the inability of the blood meter to measure L-β-hydroxybutyrate values POST KS warrants the further development of publicly available ketone measurement apparatuses. |
| first_indexed | 2024-03-08T20:28:38Z |
| format | Article |
| id | doaj.art-0a65a3f750a846228d11cc9f59fe8499 |
| institution | Directory Open Access Journal |
| issn | 2813-0413 |
| language | English |
| last_indexed | 2024-03-08T20:28:38Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Muscles |
| spelling | doaj.art-0a65a3f750a846228d11cc9f59fe84992023-12-22T14:28:52ZengMDPI AGMuscles2813-04132023-09-012432733710.3390/muscles2040025Discrepancy of Beta-Hydroxybutyrate Measurements between a Blood Meter and GC-MS Methods in Healthy HumansAngelia Maleah Holland-Winkler0Andrew R. Moore1Jenna K. Ansley2Noah A. Fritz3Ilya Bederman4Department of Kinesiology, Augusta University, Augusta, GA 30909, USADepartment of Kinesiology, Augusta University, Augusta, GA 30909, USADepartment of Kinesiology, Augusta University, Augusta, GA 30909, USADepartment of Kinesiology, Augusta University, Augusta, GA 30909, USADepartment of Genetics and Genome Sciences, School of Medicine, Case Western University, Cleveland, OH 44106, USAKetone salt (KS) supplementation induces temporary nutritional ketosis to achieve potential exercise performance and health benefits. Racemic KS includes both D/L isomers of β-hydroxybutyrate, yet commercially available measurement devices (i.e., blood meters) only measure the D variant. The aim of this study was to investigate the efficacy of a blood meter to measure serum β-hydroxybutyrate in comparison with gas chromatography–mass spectrometry (GC-MS) before and 30 min after consuming a placebo or racemic KS. In this triple-blinded cross-over study, 16 healthy adults were administered either a placebo or KS drink, and the circulating β-hydroxybutyrate concentration was measured at baseline (PRE) and 30 min following consumption (POST) using a blood ketone meter and by GC-MS. Compared to the placebo, both GC-MS and the blood meter obtained significantly greater β-hydroxybutyrate levels from PRE to POST time-points after consuming KS. Additionally, GC-MS results showed significantly higher levels of β-hydroxybutyrate with both the placebo and KS at PRE and POST time-points, as compared to the blood meter. These results indicate that (1) even in the absence of KS, the blood meter yields significantly lower β-hydroxybutyrate values than GC-MS, and (2) the inability of the blood meter to measure L-β-hydroxybutyrate values POST KS warrants the further development of publicly available ketone measurement apparatuses.https://www.mdpi.com/2813-0413/2/4/25β -hydroxybutyrateblood meterhumansketone saltsketosis |
| spellingShingle | Angelia Maleah Holland-Winkler Andrew R. Moore Jenna K. Ansley Noah A. Fritz Ilya Bederman Discrepancy of Beta-Hydroxybutyrate Measurements between a Blood Meter and GC-MS Methods in Healthy Humans Muscles β -hydroxybutyrate blood meter humans ketone salts ketosis |
| title | Discrepancy of Beta-Hydroxybutyrate Measurements between a Blood Meter and GC-MS Methods in Healthy Humans |
| title_full | Discrepancy of Beta-Hydroxybutyrate Measurements between a Blood Meter and GC-MS Methods in Healthy Humans |
| title_fullStr | Discrepancy of Beta-Hydroxybutyrate Measurements between a Blood Meter and GC-MS Methods in Healthy Humans |
| title_full_unstemmed | Discrepancy of Beta-Hydroxybutyrate Measurements between a Blood Meter and GC-MS Methods in Healthy Humans |
| title_short | Discrepancy of Beta-Hydroxybutyrate Measurements between a Blood Meter and GC-MS Methods in Healthy Humans |
| title_sort | discrepancy of beta hydroxybutyrate measurements between a blood meter and gc ms methods in healthy humans |
| topic | β -hydroxybutyrate blood meter humans ketone salts ketosis |
| url | https://www.mdpi.com/2813-0413/2/4/25 |
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