Beneficial factors for biomineralization by ureolytic bacterium Sporosarcina pasteurii
Abstract Background The ureolytic bacterium Sporosarcina pasteurii is well-known for its capability of microbially induced calcite precipitation (MICP), representing a great potential in constructional engineering and material applications. However, the molecular mechanism for its biomineralization...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-01-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12934-020-1281-z |
| _version_ | 1818844929803157504 |
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| author | Liang Ma Ai-Ping Pang Yongsheng Luo Xiaolin Lu Fengming Lin |
| author_facet | Liang Ma Ai-Ping Pang Yongsheng Luo Xiaolin Lu Fengming Lin |
| author_sort | Liang Ma |
| collection | DOAJ |
| description | Abstract Background The ureolytic bacterium Sporosarcina pasteurii is well-known for its capability of microbially induced calcite precipitation (MICP), representing a great potential in constructional engineering and material applications. However, the molecular mechanism for its biomineralization remains unresolved, as few studies were carried out. Results The addition of urea into the culture medium provided an alkaline environment that is suitable for S. pasteurii. As compared to S. pasteurii cultivated without urea, S. pasteurii grown with urea showed faster growth and urease production, better shape, more negative surface charge and higher biomineralization ability. To survive the unfavorable growth environment due to the absence of urea, S. pasteurii up-regulated the expression of genes involved in urease production, ATPase synthesis and flagella, possibly occupying resources that can be deployed for MICP. As compared to non-mineralizing bacteria, S. pasteurii exhibited more negative cell surface charge for binding calcium ions and more robust cell structure as nucleation sites. During MICP process, the genes for ATPase synthesis in S. pasteurii was up-regulated while genes for urease production were unchanged. Interestingly, genes involved in flagella were down-regulated during MICP, which might lead to poor mobility of S. pasteurii. Meanwhile, genes in fatty acid degradation pathway were inhibited to maintain the intact cell structure found in calcite precipitation. Both weak mobility and intact cell structure are advantageous for S. pasteurii to serve as nucleation sites during MICP. Conclusions Four factors are demonstrated to benefit the super performance of S. pasteurii in MICP. First, the good correlation of biomass growth and urease production of S. pasteurii provides sufficient biomass and urease simultaneously for improved biomineralization. Second, the highly negative cell surface charge of S. pasteurii is good for binding calcium ions. Third, the robust cell structure and fourth, the weak mobility, are key for S. pasteurii to be nucleation sites during MICP. |
| first_indexed | 2024-12-19T05:21:35Z |
| format | Article |
| id | doaj.art-0a663ec09f654167a26645679d22bb15 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-12-19T05:21:35Z |
| publishDate | 2020-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-0a663ec09f654167a26645679d22bb152022-12-21T20:34:29ZengBMCMicrobial Cell Factories1475-28592020-01-0119111210.1186/s12934-020-1281-zBeneficial factors for biomineralization by ureolytic bacterium Sporosarcina pasteuriiLiang Ma0Ai-Ping Pang1Yongsheng Luo2Xiaolin Lu3Fengming Lin4State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast UniversityState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast UniversityState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast UniversityState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast UniversityState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast UniversityAbstract Background The ureolytic bacterium Sporosarcina pasteurii is well-known for its capability of microbially induced calcite precipitation (MICP), representing a great potential in constructional engineering and material applications. However, the molecular mechanism for its biomineralization remains unresolved, as few studies were carried out. Results The addition of urea into the culture medium provided an alkaline environment that is suitable for S. pasteurii. As compared to S. pasteurii cultivated without urea, S. pasteurii grown with urea showed faster growth and urease production, better shape, more negative surface charge and higher biomineralization ability. To survive the unfavorable growth environment due to the absence of urea, S. pasteurii up-regulated the expression of genes involved in urease production, ATPase synthesis and flagella, possibly occupying resources that can be deployed for MICP. As compared to non-mineralizing bacteria, S. pasteurii exhibited more negative cell surface charge for binding calcium ions and more robust cell structure as nucleation sites. During MICP process, the genes for ATPase synthesis in S. pasteurii was up-regulated while genes for urease production were unchanged. Interestingly, genes involved in flagella were down-regulated during MICP, which might lead to poor mobility of S. pasteurii. Meanwhile, genes in fatty acid degradation pathway were inhibited to maintain the intact cell structure found in calcite precipitation. Both weak mobility and intact cell structure are advantageous for S. pasteurii to serve as nucleation sites during MICP. Conclusions Four factors are demonstrated to benefit the super performance of S. pasteurii in MICP. First, the good correlation of biomass growth and urease production of S. pasteurii provides sufficient biomass and urease simultaneously for improved biomineralization. Second, the highly negative cell surface charge of S. pasteurii is good for binding calcium ions. Third, the robust cell structure and fourth, the weak mobility, are key for S. pasteurii to be nucleation sites during MICP.https://doi.org/10.1186/s12934-020-1281-zSporosarcina pasteuriiBiomineralizationBacterial surface potentialUreaseATP synthase |
| spellingShingle | Liang Ma Ai-Ping Pang Yongsheng Luo Xiaolin Lu Fengming Lin Beneficial factors for biomineralization by ureolytic bacterium Sporosarcina pasteurii Microbial Cell Factories Sporosarcina pasteurii Biomineralization Bacterial surface potential Urease ATP synthase |
| title | Beneficial factors for biomineralization by ureolytic bacterium Sporosarcina pasteurii |
| title_full | Beneficial factors for biomineralization by ureolytic bacterium Sporosarcina pasteurii |
| title_fullStr | Beneficial factors for biomineralization by ureolytic bacterium Sporosarcina pasteurii |
| title_full_unstemmed | Beneficial factors for biomineralization by ureolytic bacterium Sporosarcina pasteurii |
| title_short | Beneficial factors for biomineralization by ureolytic bacterium Sporosarcina pasteurii |
| title_sort | beneficial factors for biomineralization by ureolytic bacterium sporosarcina pasteurii |
| topic | Sporosarcina pasteurii Biomineralization Bacterial surface potential Urease ATP synthase |
| url | https://doi.org/10.1186/s12934-020-1281-z |
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