RAPID DNA EXTRACTION AND PCR VALIDATION FOR DIRECT DETECTION OF Listeria monocytogenes IN RAW MILK

RAPID DNA EXTRACTION AND PCR VALIDATION FOR DIRECT DETECTION OF Listeria monocytogenes IN RAW MILK

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Objective. The aim of this study was to validate a method for detecting L. monocytogenes in raw milk.Materials and methods. The extraction procedure carried out using a chaotropic agent like NaI, toreduce fat in the sample to 0.2% w/v, which is the lowest limit for detection in the Gerber method, to...

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Bibliographic Details
Main Authors: Edith Burbano, Sara Sierra, Kirvis Torres, Marcela Mercado, Ana Carrascal, Raúl Poutou
Format: Article
Language:English
Published: Universidad de Cordoba 2006-05-01
Series:Revista MVZ Cordoba
Subjects:
Listeria monocytogenes
PCR
Validation
Raw milk.
Online Access:http://apps.unicordoba.edu.co/revistas/revistamvz/mvz-111/111-5.pdf

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