HPLC method development and validation for the estimation of axitinibe in rabbit plasma
ABSTRACT A rapid, sensitive, and accurate high performance liquid chromatography for the determination of axitinibe (AN) in rabbit plasma is developed using crizotinibe as an internal standard (IS). Axitinibe is a tyrosine kinase inhibitor, used in the treatment of advanced kidney cancer, which work...
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2017-10-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300607&lng=en&tlng=en |
| _version_ | 1818350904064081920 |
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| author | Achanta Suneetha Sharmila Donepudi |
| author_facet | Achanta Suneetha Sharmila Donepudi |
| author_sort | Achanta Suneetha |
| collection | DOAJ |
| description | ABSTRACT A rapid, sensitive, and accurate high performance liquid chromatography for the determination of axitinibe (AN) in rabbit plasma is developed using crizotinibe as an internal standard (IS). Axitinibe is a tyrosine kinase inhibitor, used in the treatment of advanced kidney cancer, which works by slowing or stopping the growth of cancer cells. The chromatographic separation was performed on a Waters 2695, Kromosil (150 mm × 4.6 mm, 5 µm) column using a mobile phase containing buffer (pH 4.6) and acetonitrile in the ratio of 65:35 v/v with a flow rate of1 mL/min. The analyte and internal standard were extracted using liquid-liquid extraction with acetonitrile. The elution was detected by photo diode array detector at 320 nm.The total chromatographic runtime is 10.0 min with a retention time for axitinibe and IS of 5.685, and 3.606 min, respectively. The method was validated over a dynamic linear range of 0.002-0.2µg/mL for axitinibe with a correlation coefficient of r2 0.999. |
| first_indexed | 2024-12-13T18:29:15Z |
| format | Article |
| id | doaj.art-0a695ff7946949f78408e1333d0b80ac |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-12-13T18:29:15Z |
| publishDate | 2017-10-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-0a695ff7946949f78408e1333d0b80ac2022-12-21T23:35:32ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902017-10-0153310.1590/s2175-97902017000300012S1984-82502017000300607HPLC method development and validation for the estimation of axitinibe in rabbit plasmaAchanta SuneethaSharmila DonepudiABSTRACT A rapid, sensitive, and accurate high performance liquid chromatography for the determination of axitinibe (AN) in rabbit plasma is developed using crizotinibe as an internal standard (IS). Axitinibe is a tyrosine kinase inhibitor, used in the treatment of advanced kidney cancer, which works by slowing or stopping the growth of cancer cells. The chromatographic separation was performed on a Waters 2695, Kromosil (150 mm × 4.6 mm, 5 µm) column using a mobile phase containing buffer (pH 4.6) and acetonitrile in the ratio of 65:35 v/v with a flow rate of1 mL/min. The analyte and internal standard were extracted using liquid-liquid extraction with acetonitrile. The elution was detected by photo diode array detector at 320 nm.The total chromatographic runtime is 10.0 min with a retention time for axitinibe and IS of 5.685, and 3.606 min, respectively. The method was validated over a dynamic linear range of 0.002-0.2µg/mL for axitinibe with a correlation coefficient of r2 0.999.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300607&lng=en&tlng=enHigh performance liquid chromatography/method validationAxitinibe/determinationAxitinibe/ kidney cancer treatmentCrizotinibeRabbit plasmaTyrosine kinase inhibitor |
| spellingShingle | Achanta Suneetha Sharmila Donepudi HPLC method development and validation for the estimation of axitinibe in rabbit plasma Brazilian Journal of Pharmaceutical Sciences High performance liquid chromatography/method validation Axitinibe/determination Axitinibe/ kidney cancer treatment Crizotinibe Rabbit plasma Tyrosine kinase inhibitor |
| title | HPLC method development and validation for the estimation of axitinibe in rabbit plasma |
| title_full | HPLC method development and validation for the estimation of axitinibe in rabbit plasma |
| title_fullStr | HPLC method development and validation for the estimation of axitinibe in rabbit plasma |
| title_full_unstemmed | HPLC method development and validation for the estimation of axitinibe in rabbit plasma |
| title_short | HPLC method development and validation for the estimation of axitinibe in rabbit plasma |
| title_sort | hplc method development and validation for the estimation of axitinibe in rabbit plasma |
| topic | High performance liquid chromatography/method validation Axitinibe/determination Axitinibe/ kidney cancer treatment Crizotinibe Rabbit plasma Tyrosine kinase inhibitor |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300607&lng=en&tlng=en |
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