MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum
Summary: Comet assay is a standard approach for studying DNA damage in malaria, but high-throughput options are not available. The CometChip was previously developed using mammalian cells as a high-throughput version of the comet assay. It is based on the same principle as the comet assay but provid...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721005037 |
| _version_ | 1818918242313306112 |
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| author | Aoli Xiong Simran Kaushal Ian Junjie Tay Bevin P. Engelward Jongyoon Han Peter R. Preiser |
| author_facet | Aoli Xiong Simran Kaushal Ian Junjie Tay Bevin P. Engelward Jongyoon Han Peter R. Preiser |
| author_sort | Aoli Xiong |
| collection | DOAJ |
| description | Summary: Comet assay is a standard approach for studying DNA damage in malaria, but high-throughput options are not available. The CometChip was previously developed using mammalian cells as a high-throughput version of the comet assay. It is based on the same principle as the comet assay but provides greater efficacy, automated data processing, and improved consistency between experiments. In this protocol, we present MalariaCometChip to quantitatively assess drug-induced DNA damage in Plasmodium falciparum.For complete details on the use and execution of this protocol, please refer to Xiong et al. (2020). |
| first_indexed | 2024-12-20T00:46:51Z |
| format | Article |
| id | doaj.art-0a6b0d38982740a2bba334d1bd35f6eb |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-20T00:46:51Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-0a6b0d38982740a2bba334d1bd35f6eb2022-12-21T19:59:23ZengElsevierSTAR Protocols2666-16672021-09-0123100797MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparumAoli Xiong0Simran Kaushal1Ian Junjie Tay2Bevin P. Engelward3Jongyoon Han4Peter R. Preiser5School of Biological Sciences, Nanyang Technological University, Singapore 639798, Singapore; BioSystems and Micromechanics (BioSyM) interdisciplinary research group (IRG), Singapore-MIT Alliance for Research and Technology (SMART), Singapore 138602, Singapore; Anti-Microbial Resistance (AMR) IRG, Singapore-MIT Alliance for Research and Technology (SMART), Singapore 138602, SingaporeDepartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USADepartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USADepartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USABioSystems and Micromechanics (BioSyM) interdisciplinary research group (IRG), Singapore-MIT Alliance for Research and Technology (SMART), Singapore 138602, Singapore; Anti-Microbial Resistance (AMR) IRG, Singapore-MIT Alliance for Research and Technology (SMART), Singapore 138602, Singapore; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Electrical Engineering & Computer Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USASchool of Biological Sciences, Nanyang Technological University, Singapore 639798, Singapore; Anti-Microbial Resistance (AMR) IRG, Singapore-MIT Alliance for Research and Technology (SMART), Singapore 138602, Singapore; Corresponding authorSummary: Comet assay is a standard approach for studying DNA damage in malaria, but high-throughput options are not available. The CometChip was previously developed using mammalian cells as a high-throughput version of the comet assay. It is based on the same principle as the comet assay but provides greater efficacy, automated data processing, and improved consistency between experiments. In this protocol, we present MalariaCometChip to quantitatively assess drug-induced DNA damage in Plasmodium falciparum.For complete details on the use and execution of this protocol, please refer to Xiong et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166721005037High-Throughput ScreeningMicrobiologyMicroscopyMolecular BiologyBiotechnology and bioengineering |
| spellingShingle | Aoli Xiong Simran Kaushal Ian Junjie Tay Bevin P. Engelward Jongyoon Han Peter R. Preiser MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum STAR Protocols High-Throughput Screening Microbiology Microscopy Molecular Biology Biotechnology and bioengineering |
| title | MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum |
| title_full | MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum |
| title_fullStr | MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum |
| title_full_unstemmed | MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum |
| title_short | MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum |
| title_sort | malariacometchip for high throughput quantification of dna damage in plasmodium falciparum |
| topic | High-Throughput Screening Microbiology Microscopy Molecular Biology Biotechnology and bioengineering |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721005037 |
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