Analysis of <i>YUC</i> and <i>TAA/TAR</i> Gene Families in Tomato
Auxin is a vital phytohormone, but its synthesis pathway is poorly understood. This study used bioinformatic analysis to identify and analyze the gene family members that encode tomato auxin biosynthesis. The <i>FZY</i> gene family members encoding flavin-containing monooxygenases were r...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/6/665 |
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| author | Sida Meng Hengzuo Xiang Xiaoru Yang Yunzhu Ye Yuying Ma Leilei Han Tao Xu Yufeng Liu Feng Wang Mingfang Qi Tianlai Li |
| author_facet | Sida Meng Hengzuo Xiang Xiaoru Yang Yunzhu Ye Yuying Ma Leilei Han Tao Xu Yufeng Liu Feng Wang Mingfang Qi Tianlai Li |
| author_sort | Sida Meng |
| collection | DOAJ |
| description | Auxin is a vital phytohormone, but its synthesis pathway is poorly understood. This study used bioinformatic analysis to identify and analyze the gene family members that encode tomato auxin biosynthesis. The <i>FZY</i> gene family members encoding flavin-containing monooxygenases were retrieved from the tomato genome database. DNAMAN analysis revealed nine genes within the landmark domain WL(I/V)VATGENAE, between the FAD and NADPH domains. Phylogenetic analysis showed that the <i>FZY</i> gene family in tomato is closely related to the <i>YUC</i> gene family in <i>Arabidopsis thaliana</i>. A qRT-PCR showed that <i>SlFZY2</i>, <i>SlFZY3</i>, <i>SlFZY4-1</i>, and <i>SlFZY5</i> were highly expressed in tomato flower organs. The analysis of promoter cis-acting elements revealed light-responsive elements in the promoters of all nine members in tomato, indicating their sensitivity to light signals. Furthermore, the promoters of <i>SlFZY4-2</i>, <i>SlFZY5</i>, and <i>SlFZY7</i> contain low-temperature-responsive elements. This study demonstrated that <i>SlTAA5</i> expression was 2.22 times that of <i>SlTAA3</i> in the roots, and <i>SlTAA3</i> expression in the pistils was 83.58 times that in the stamens during the tomato flowering stage. Therefore, various members of the tomato <i>FZY</i> gene family are involved in regulating the development of tomato floral organs and are responsive to abiotic stresses, such as low temperature and weak light. |
| first_indexed | 2024-03-11T02:23:07Z |
| format | Article |
| id | doaj.art-0a6b513d023b479a8a8b17b834934441 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-11T02:23:07Z |
| publishDate | 2023-06-01 |
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| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-0a6b513d023b479a8a8b17b8349344412023-11-18T10:40:56ZengMDPI AGHorticulturae2311-75242023-06-019666510.3390/horticulturae9060665Analysis of <i>YUC</i> and <i>TAA/TAR</i> Gene Families in TomatoSida Meng0Hengzuo Xiang1Xiaoru Yang2Yunzhu Ye3Yuying Ma4Leilei Han5Tao Xu6Yufeng Liu7Feng Wang8Mingfang Qi9Tianlai Li10College of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaAuxin is a vital phytohormone, but its synthesis pathway is poorly understood. This study used bioinformatic analysis to identify and analyze the gene family members that encode tomato auxin biosynthesis. The <i>FZY</i> gene family members encoding flavin-containing monooxygenases were retrieved from the tomato genome database. DNAMAN analysis revealed nine genes within the landmark domain WL(I/V)VATGENAE, between the FAD and NADPH domains. Phylogenetic analysis showed that the <i>FZY</i> gene family in tomato is closely related to the <i>YUC</i> gene family in <i>Arabidopsis thaliana</i>. A qRT-PCR showed that <i>SlFZY2</i>, <i>SlFZY3</i>, <i>SlFZY4-1</i>, and <i>SlFZY5</i> were highly expressed in tomato flower organs. The analysis of promoter cis-acting elements revealed light-responsive elements in the promoters of all nine members in tomato, indicating their sensitivity to light signals. Furthermore, the promoters of <i>SlFZY4-2</i>, <i>SlFZY5</i>, and <i>SlFZY7</i> contain low-temperature-responsive elements. This study demonstrated that <i>SlTAA5</i> expression was 2.22 times that of <i>SlTAA3</i> in the roots, and <i>SlTAA3</i> expression in the pistils was 83.58 times that in the stamens during the tomato flowering stage. Therefore, various members of the tomato <i>FZY</i> gene family are involved in regulating the development of tomato floral organs and are responsive to abiotic stresses, such as low temperature and weak light.https://www.mdpi.com/2311-7524/9/6/665tomatoauxin<i>YUC</i> gene family<i>TAA/TAR</i> gene familybioinformatics |
| spellingShingle | Sida Meng Hengzuo Xiang Xiaoru Yang Yunzhu Ye Yuying Ma Leilei Han Tao Xu Yufeng Liu Feng Wang Mingfang Qi Tianlai Li Analysis of <i>YUC</i> and <i>TAA/TAR</i> Gene Families in Tomato Horticulturae tomato auxin <i>YUC</i> gene family <i>TAA/TAR</i> gene family bioinformatics |
| title | Analysis of <i>YUC</i> and <i>TAA/TAR</i> Gene Families in Tomato |
| title_full | Analysis of <i>YUC</i> and <i>TAA/TAR</i> Gene Families in Tomato |
| title_fullStr | Analysis of <i>YUC</i> and <i>TAA/TAR</i> Gene Families in Tomato |
| title_full_unstemmed | Analysis of <i>YUC</i> and <i>TAA/TAR</i> Gene Families in Tomato |
| title_short | Analysis of <i>YUC</i> and <i>TAA/TAR</i> Gene Families in Tomato |
| title_sort | analysis of i yuc i and i taa tar i gene families in tomato |
| topic | tomato auxin <i>YUC</i> gene family <i>TAA/TAR</i> gene family bioinformatics |
| url | https://www.mdpi.com/2311-7524/9/6/665 |
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